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Q51D88 (PAP_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase

Short name=PAP
EC=2.7.7.19
Alternative name(s):
EhPAP
Polynucleotide adenylyltransferase
Gene names
ORF Names:16.t00038
OrganismEntamoeba histolytica [Reference proteome]
Taxonomic identifier5759 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor. Ref.1

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subcellular location

Cytoplasm. Nucleus Ref.1.

Developmental stage

Mainly expressed during G1 and S phases. Ref.1

Sequence similarities

Belongs to the poly(A) polymerase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Poly(A) polymerase
PRO_0000051617

Regions

Nucleotide binding63 – 653ATP By similarity
Nucleotide binding76 – 783ATP By similarity
Nucleotide binding211 – 2122ATP By similarity

Sites

Metal binding761Magnesium 1; catalytic By similarity
Metal binding761Magnesium 2; catalytic By similarity
Metal binding781Magnesium 1; catalytic By similarity
Metal binding781Magnesium 2; catalytic By similarity
Metal binding1301Magnesium 2; catalytic By similarity
Binding site1301ATP By similarity
Binding site1931ATP By similarity
Binding site2021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q51D88 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: B4A546672B639CBF

FASTA52259,810
        10         20         30         40         50         60 
MACELKEFLV KNDMYPMGDQ VEKKRKAISK MTEYIQQWGK KIYIESAGVA DDTDVKAATI 

        70         80         90        100        110        120 
YVYGSYRLNV YGNNSDIDAC IVSNSTITRD DFYDGLYAEL LNNPDVKELK QIPSKRSPHL 

       130        140        150        160        170        180 
SMIYLNIEFD LNFSRTAYTS LPDNLDILNE NILKNMDELD TRAINGVRNT DIIDAFVPNH 

       190        200        210        220        230        240 
SEEAFRVMVR TIKLWTKKRG IYGYVYCFLN GISIEILVAQ VISENYQLDN VRLLEKFFQV 

       250        260        270        280        290        300 
YSSWDWLRTP VMLGTNDDFN DKKKEGVIQI LTPASPSENA AFSITKFSLE MIKRELKRGQ 

       310        320        330        340        350        360 
EIVHEFSSEG VNDWAKLFKP RHLFCGYYIF IEFIVTSSSL EGLTTTIGKF ESGLVNLMKG 

       370        380        390        400        410        420 
LSEIEEIIEA NVIPNGFLDE ENEKYFYYVG MNVKRDCPVD ISTPLNSFLS IVNSGKDLIV 

       430        440        450        460        470        480 
DASIKKRSEI PTKFAHSVKR SITKSQEIKE TSSQVPSSAI TETFDIPTKP SIEQQLKAKE 

       490        500        510        520 
ENSIPNEEKK EQLKKEMKQE ANTIVKNSST DDDFMKRFTR KN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS571148 Genomic DNA. Translation: EAL50821.1.
RefSeqXP_656205.1. XM_651113.1.

3D structure databases

ProteinModelPortalQ51D88.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING294381.XP_656205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3410521.
KEGGehi:EHI_012040.

Organism-specific databases

EuPathDBAmoebaDB:EHI_012040.

Phylogenomic databases

eggNOGCOG5186.
InParanoidQ51D88.
KOK14376.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
[Graphical view]
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Entry information

Entry namePAP_ENTHI
AccessionPrimary (citable) accession number: Q51D88
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families