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Q51D88

- PAP_ENTHI

UniProt

Q51D88 - PAP_ENTHI

Protein

Poly(A) polymerase

Gene

16.t00038

Organism
Entamoeba histolytica
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor.1 Publication

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi76 – 761Magnesium 1; catalyticBy similarity
    Metal bindingi76 – 761Magnesium 2; catalyticBy similarity
    Metal bindingi78 – 781Magnesium 1; catalyticBy similarity
    Metal bindingi78 – 781Magnesium 2; catalyticBy similarity
    Metal bindingi130 – 1301Magnesium 2; catalyticBy similarity
    Binding sitei130 – 1301ATPBy similarity
    Binding sitei193 – 1931ATPBy similarity
    Binding sitei202 – 2021ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi63 – 653ATPBy similarity
    Nucleotide bindingi76 – 783ATPBy similarity
    Nucleotide bindingi211 – 2122ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
    4. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. RNA polyadenylation Source: InterPro

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase (EC:2.7.7.19)
    Short name:
    PAP
    Alternative name(s):
    EhPAP
    Polynucleotide adenylyltransferase
    Gene namesi
    ORF Names:16.t00038
    OrganismiEntamoeba histolytica
    Taxonomic identifieri5759 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
    ProteomesiUP000001926: Partially assembled WGS sequence

    Organism-specific databases

    EuPathDBiAmoebaDB:EHI_012040.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 522522Poly(A) polymerasePRO_0000051617Add
    BLAST

    Expressioni

    Developmental stagei

    Mainly expressed during G1 and S phases.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi294381.XP_656205.

    Structurei

    3D structure databases

    ProteinModelPortaliQ51D88.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiCOG5186.
    InParanoidiQ51D88.
    KOiK14376.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    [Graphical view]
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q51D88-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACELKEFLV KNDMYPMGDQ VEKKRKAISK MTEYIQQWGK KIYIESAGVA    50
    DDTDVKAATI YVYGSYRLNV YGNNSDIDAC IVSNSTITRD DFYDGLYAEL 100
    LNNPDVKELK QIPSKRSPHL SMIYLNIEFD LNFSRTAYTS LPDNLDILNE 150
    NILKNMDELD TRAINGVRNT DIIDAFVPNH SEEAFRVMVR TIKLWTKKRG 200
    IYGYVYCFLN GISIEILVAQ VISENYQLDN VRLLEKFFQV YSSWDWLRTP 250
    VMLGTNDDFN DKKKEGVIQI LTPASPSENA AFSITKFSLE MIKRELKRGQ 300
    EIVHEFSSEG VNDWAKLFKP RHLFCGYYIF IEFIVTSSSL EGLTTTIGKF 350
    ESGLVNLMKG LSEIEEIIEA NVIPNGFLDE ENEKYFYYVG MNVKRDCPVD 400
    ISTPLNSFLS IVNSGKDLIV DASIKKRSEI PTKFAHSVKR SITKSQEIKE 450
    TSSQVPSSAI TETFDIPTKP SIEQQLKAKE ENSIPNEEKK EQLKKEMKQE 500
    ANTIVKNSST DDDFMKRFTR KN 522
    Length:522
    Mass (Da):59,810
    Last modified:June 7, 2005 - v1
    Checksum:iB4A546672B639CBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS571148 Genomic DNA. Translation: EAL50821.1.
    RefSeqiXP_656205.1. XM_651113.1.

    Genome annotation databases

    GeneIDi3410521.
    KEGGiehi:EHI_012040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DS571148 Genomic DNA. Translation: EAL50821.1 .
    RefSeqi XP_656205.1. XM_651113.1.

    3D structure databases

    ProteinModelPortali Q51D88.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 294381.XP_656205.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3410521.
    KEGGi ehi:EHI_012040.

    Organism-specific databases

    EuPathDBi AmoebaDB:EHI_012040.

    Phylogenomic databases

    eggNOGi COG5186.
    InParanoidi Q51D88.
    KOi K14376.

    Family and domain databases

    Gene3Di 3.30.70.590. 1 hit.
    InterProi IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55003. SSF55003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Entamoeba histolytica: cloning and expression of the poly(A) polymerase EhPAP."
      Garcia-Vivas J., Lopez-Camarillo C., Azuara-Liceaga E., Orozco E., Marchat L.A.
      Exp. Parasitol. 110:226-232(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 30459 / HM-1:IMSS.
    3. Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: ATCC 30459 / HM-1:IMSS.

    Entry informationi

    Entry nameiPAP_ENTHI
    AccessioniPrimary (citable) accession number: Q51D88
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3