Q51D88 (PAP_ENTHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase
Short name=PAP
EC=2.7.7.19

Alternative name(s):
EhPAP
Polynucleotide adenylyltransferase

Gene names

ORF Names:

16.t00038

Organism

Entamoeba histolytica [Reference proteome]

Taxonomic identifier

5759 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Archamoebae › Entamoebidae › Entamoeba

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor. Ref.1
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Binds 2 magnesium ions. Also active with manganese By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.1.
Developmental stage	Mainly expressed during G1 and S phases. Ref.1
Sequence similarities	Belongs to the poly(A) polymerase family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA polyadenylation Inferred from electronic annotation. Source: InterPro mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Poly(A) polymerase		PRO_0000051617
Regions
	Nucleotide binding	63 – 65	3	ATP By similarity
	Nucleotide binding	76 – 78	3	ATP By similarity
	Nucleotide binding	211 – 212	2	ATP By similarity
Sites
	Metal binding	76	1	Magnesium 1; catalytic By similarity
	Metal binding	76	1	Magnesium 2; catalytic By similarity
	Metal binding	78	1	Magnesium 1; catalytic By similarity
	Metal binding	78	1	Magnesium 2; catalytic By similarity
	Metal binding	130	1	Magnesium 2; catalytic By similarity
	Binding site	130	1	ATP By similarity
	Binding site	193	1	ATP By similarity
	Binding site	202	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51D88 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: B4A546672B639CBF	FASTA	522	59,810
	10 20 30 40 50 60 MACELKEFLV KNDMYPMGDQ VEKKRKAISK MTEYIQQWGK KIYIESAGVA DDTDVKAATI 70 80 90 100 110 120 YVYGSYRLNV YGNNSDIDAC IVSNSTITRD DFYDGLYAEL LNNPDVKELK QIPSKRSPHL 130 140 150 160 170 180 SMIYLNIEFD LNFSRTAYTS LPDNLDILNE NILKNMDELD TRAINGVRNT DIIDAFVPNH 190 200 210 220 230 240 SEEAFRVMVR TIKLWTKKRG IYGYVYCFLN GISIEILVAQ VISENYQLDN VRLLEKFFQV 250 260 270 280 290 300 YSSWDWLRTP VMLGTNDDFN DKKKEGVIQI LTPASPSENA AFSITKFSLE MIKRELKRGQ 310 320 330 340 350 360 EIVHEFSSEG VNDWAKLFKP RHLFCGYYIF IEFIVTSSSL EGLTTTIGKF ESGLVNLMKG 370 380 390 400 410 420 LSEIEEIIEA NVIPNGFLDE ENEKYFYYVG MNVKRDCPVD ISTPLNSFLS IVNSGKDLIV 430 440 450 460 470 480 DASIKKRSEI PTKFAHSVKR SITKSQEIKE TSSQVPSSAI TETFDIPTKP SIEQQLKAKE 490 500 510 520 ENSIPNEEKK EQLKKEMKQE ANTIVKNSST DDDFMKRFTR KN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Entamoeba histolytica: cloning and expression of the poly(A) polymerase EhPAP." Garcia-Vivas J., Lopez-Camarillo C., Azuara-Liceaga E., Orozco E., Marchat L.A. Exp. Parasitol. 110:226-232(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
[2]	"The genome of the protist parasite Entamoeba histolytica." Loftus B.J., Anderson I., Davies R., Alsmark U.C., Samuelson J., Amedeo P., Roncaglia P., Berriman M., Hirt R.P., Mann B.J., Nozaki T., Suh B., Pop M., Duchene M., Ackers J., Tannich E., Leippe M., Hofer M. , Bruchhaus I., Willhoeft U., Bhattacharya A., Chillingworth T., Churcher C.M., Hance Z., Harris B., Harris D., Jagels K., Moule S., Mungall K.L., Ormond D., Squares R., Whitehead S., Quail M.A., Rabbinowitsch E., Norbertczak H., Price C., Wang Z., Guillen N., Gilchrist C., Stroup S.E., Bhattacharya S., Lohia A., Foster P.G., Sicheritz-Ponten T., Weber C., Singh U., Mukherjee C., El-Sayed N.M.A., Petri W.A., Clark C.G., Embley T.M., Barrell B.G., Fraser C.M., Hall N. Nature 433:865-868(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 30459 / HM-1:IMSS.
[3]	Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 30459 / HM-1:IMSS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS571148 Genomic DNA. Translation: EAL50821.1.
RefSeq	XP_656205.1. XM_651113.1.
3D structure databases
ProteinModelPortal	Q51D88.
ModBase	Search...
Protein-protein interaction databases
STRING	294381.XP_656205.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	rna_EHI_012040-1; rna_EHI_012040-1; EHI_012040.
GeneID	3410521.
KEGG	ehi:EHI_012040.
Organism-specific databases
EuPathDB	AmoebaDB:EHI_012040.
Phylogenomic databases
eggNOG	COG5186.
InParanoid	Q51D88.
KO	K14376.
ProtClustDB	CLSZ2732636.
Family and domain databases
Gene3D	3.30.70.590. 1 hit.
InterPro	IPR002934. Nucleotidyltransferase. IPR011068. NuclTrfase_I_C. IPR007012. PolA_pol_cen_dom. IPR007010. PolA_pol_RNA-bd_dom. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF04928. PAP_central. 1 hit. [Graphical view]
SUPFAM	SSF55003. PAP_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAP_ENTHI

Accession

Primary (citable) accession number: Q51D88

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)