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Q51D88

- PAP_ENTHI

UniProt

Q51D88 - PAP_ENTHI

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Protein
Poly(A) polymerase
Gene
16.t00038
Organism
Entamoeba histolytica
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Magnesium 1; catalytic By similarity
Metal bindingi76 – 761Magnesium 2; catalytic By similarity
Metal bindingi78 – 781Magnesium 1; catalytic By similarity
Metal bindingi78 – 781Magnesium 2; catalytic By similarity
Metal bindingi130 – 1301Magnesium 2; catalytic By similarity
Binding sitei130 – 1301ATP By similarity
Binding sitei193 – 1931ATP By similarity
Binding sitei202 – 2021ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 653ATP By similarity
Nucleotide bindingi76 – 783ATP By similarity
Nucleotide bindingi211 – 2122ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. RNA polyadenylation Source: InterPro
  2. mRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
EhPAP
Polynucleotide adenylyltransferase
Gene namesi
ORF Names:16.t00038
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
ProteomesiUP000001926: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiAmoebaDB:EHI_012040.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Poly(A) polymerase
PRO_0000051617Add
BLAST

Expressioni

Developmental stagei

Mainly expressed during G1 and S phases.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi294381.XP_656205.

Structurei

3D structure databases

ProteinModelPortaliQ51D88.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5186.
InParanoidiQ51D88.
KOiK14376.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
[Graphical view]
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q51D88-1 [UniParc]FASTAAdd to Basket

« Hide

MACELKEFLV KNDMYPMGDQ VEKKRKAISK MTEYIQQWGK KIYIESAGVA    50
DDTDVKAATI YVYGSYRLNV YGNNSDIDAC IVSNSTITRD DFYDGLYAEL 100
LNNPDVKELK QIPSKRSPHL SMIYLNIEFD LNFSRTAYTS LPDNLDILNE 150
NILKNMDELD TRAINGVRNT DIIDAFVPNH SEEAFRVMVR TIKLWTKKRG 200
IYGYVYCFLN GISIEILVAQ VISENYQLDN VRLLEKFFQV YSSWDWLRTP 250
VMLGTNDDFN DKKKEGVIQI LTPASPSENA AFSITKFSLE MIKRELKRGQ 300
EIVHEFSSEG VNDWAKLFKP RHLFCGYYIF IEFIVTSSSL EGLTTTIGKF 350
ESGLVNLMKG LSEIEEIIEA NVIPNGFLDE ENEKYFYYVG MNVKRDCPVD 400
ISTPLNSFLS IVNSGKDLIV DASIKKRSEI PTKFAHSVKR SITKSQEIKE 450
TSSQVPSSAI TETFDIPTKP SIEQQLKAKE ENSIPNEEKK EQLKKEMKQE 500
ANTIVKNSST DDDFMKRFTR KN 522
Length:522
Mass (Da):59,810
Last modified:June 7, 2005 - v1
Checksum:iB4A546672B639CBF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS571148 Genomic DNA. Translation: EAL50821.1.
RefSeqiXP_656205.1. XM_651113.1.

Genome annotation databases

GeneIDi3410521.
KEGGiehi:EHI_012040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS571148 Genomic DNA. Translation: EAL50821.1 .
RefSeqi XP_656205.1. XM_651113.1.

3D structure databases

ProteinModelPortali Q51D88.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 294381.XP_656205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3410521.
KEGGi ehi:EHI_012040.

Organism-specific databases

EuPathDBi AmoebaDB:EHI_012040.

Phylogenomic databases

eggNOGi COG5186.
InParanoidi Q51D88.
KOi K14376.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
[Graphical view ]
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Entamoeba histolytica: cloning and expression of the poly(A) polymerase EhPAP."
    Garcia-Vivas J., Lopez-Camarillo C., Azuara-Liceaga E., Orozco E., Marchat L.A.
    Exp. Parasitol. 110:226-232(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 30459 / HM-1:IMSS.
  3. Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 30459 / HM-1:IMSS.

Entry informationi

Entry nameiPAP_ENTHI
AccessioniPrimary (citable) accession number: Q51D88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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