Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Poly(A) polymerase

Gene

16.t00038

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Magnesium 1; catalyticBy similarity
Metal bindingi76 – 761Magnesium 2; catalyticBy similarity
Metal bindingi78 – 781Magnesium 1; catalyticBy similarity
Metal bindingi78 – 781Magnesium 2; catalyticBy similarity
Metal bindingi130 – 1301Magnesium 2; catalyticBy similarity
Binding sitei130 – 1301ATPBy similarity
Binding sitei193 – 1931ATPBy similarity
Binding sitei202 – 2021ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 653ATPBy similarity
Nucleotide bindingi76 – 783ATPBy similarity
Nucleotide bindingi211 – 2122ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. RNA polyadenylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
EhPAP
Polynucleotide adenylyltransferase
Gene namesi
ORF Names:16.t00038
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
ProteomesiUP000001926 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiAmoebaDB:EHI_012040.

Subcellular locationi

  1. Cytoplasm 1 Publication
  2. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Poly(A) polymerasePRO_0000051617Add
BLAST

Expressioni

Developmental stagei

Mainly expressed during G1 and S phases.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi294381.XP_656205.

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiCOG5186.
InParanoidiQ51D88.
KOiK14376.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
[Graphical view]
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q51D88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACELKEFLV KNDMYPMGDQ VEKKRKAISK MTEYIQQWGK KIYIESAGVA
60 70 80 90 100
DDTDVKAATI YVYGSYRLNV YGNNSDIDAC IVSNSTITRD DFYDGLYAEL
110 120 130 140 150
LNNPDVKELK QIPSKRSPHL SMIYLNIEFD LNFSRTAYTS LPDNLDILNE
160 170 180 190 200
NILKNMDELD TRAINGVRNT DIIDAFVPNH SEEAFRVMVR TIKLWTKKRG
210 220 230 240 250
IYGYVYCFLN GISIEILVAQ VISENYQLDN VRLLEKFFQV YSSWDWLRTP
260 270 280 290 300
VMLGTNDDFN DKKKEGVIQI LTPASPSENA AFSITKFSLE MIKRELKRGQ
310 320 330 340 350
EIVHEFSSEG VNDWAKLFKP RHLFCGYYIF IEFIVTSSSL EGLTTTIGKF
360 370 380 390 400
ESGLVNLMKG LSEIEEIIEA NVIPNGFLDE ENEKYFYYVG MNVKRDCPVD
410 420 430 440 450
ISTPLNSFLS IVNSGKDLIV DASIKKRSEI PTKFAHSVKR SITKSQEIKE
460 470 480 490 500
TSSQVPSSAI TETFDIPTKP SIEQQLKAKE ENSIPNEEKK EQLKKEMKQE
510 520
ANTIVKNSST DDDFMKRFTR KN
Length:522
Mass (Da):59,810
Last modified:June 7, 2005 - v1
Checksum:iB4A546672B639CBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS571148 Genomic DNA. Translation: EAL50821.1.
RefSeqiXP_656205.1. XM_651113.1.

Genome annotation databases

GeneIDi3410521.
KEGGiehi:EHI_012040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS571148 Genomic DNA. Translation: EAL50821.1.
RefSeqiXP_656205.1. XM_651113.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi294381.XP_656205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3410521.
KEGGiehi:EHI_012040.

Organism-specific databases

EuPathDBiAmoebaDB:EHI_012040.

Phylogenomic databases

eggNOGiCOG5186.
InParanoidiQ51D88.
KOiK14376.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
[Graphical view]
SUPFAMiSSF55003. SSF55003. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Entamoeba histolytica: cloning and expression of the poly(A) polymerase EhPAP."
    Garcia-Vivas J., Lopez-Camarillo C., Azuara-Liceaga E., Orozco E., Marchat L.A.
    Exp. Parasitol. 110:226-232(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 30459 / HM-1:IMSS.
  3. Lorenzi H., Amedeo P., Inman J., Schobel S., Caler E.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 30459 / HM-1:IMSS.

Entry informationi

Entry nameiPAP_ENTHI
AccessioniPrimary (citable) accession number: Q51D88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: June 7, 2005
Last modified: April 1, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.