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Protein

Poly(A) polymerase

Gene

16.t00038

Organism
Entamoeba histolytica
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi76Magnesium 1; catalyticBy similarity1
Metal bindingi76Magnesium 2; catalyticBy similarity1
Metal bindingi78Magnesium 1; catalyticBy similarity1
Metal bindingi78Magnesium 2; catalyticBy similarity1
Metal bindingi130Magnesium 2; catalyticBy similarity1
Binding sitei130ATPBy similarity1
Binding sitei193ATPBy similarity1
Binding sitei202ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 65ATPBy similarity3
Nucleotide bindingi76 – 78ATPBy similarity3
Nucleotide bindingi211 – 212ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase (EC:2.7.7.19)
Short name:
PAP
Alternative name(s):
EhPAP
Polynucleotide adenylyltransferase
Gene namesi
ORF Names:16.t00038
OrganismiEntamoeba histolytica
Taxonomic identifieri5759 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaArchamoebaeEntamoebidaeEntamoeba
Proteomesi
  • UP000001926 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiAmoebaDB:EHI_012040

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516171 – 522Poly(A) polymeraseAdd BLAST522

Proteomic databases

PRIDEiQ51D88

Expressioni

Developmental stagei

Mainly expressed during G1 and S phases.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi5759.rna_EHI_012040-1

Structurei

3D structure databases

ProteinModelPortaliQ51D88
SMRiQ51D88
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiKOG2245 Eukaryota
COG5186 LUCA
InParanoidiQ51D88
KOiK14376
OMAiMGIVVRH

Family and domain databases

InterProiView protein in InterPro
IPR011068 NuclTrfase_I-like_C
IPR007012 PolA_pol_cen_dom
IPR014492 PolyA_polymerase
IPR002934 Polymerase_NTP_transf_dom
PANTHERiPTHR10682 PTHR10682, 1 hit
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF04928 PAP_central, 1 hit
SUPFAMiSSF55003 SSF55003, 1 hit

Sequencei

Sequence statusi: Complete.

Q51D88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACELKEFLV KNDMYPMGDQ VEKKRKAISK MTEYIQQWGK KIYIESAGVA
60 70 80 90 100
DDTDVKAATI YVYGSYRLNV YGNNSDIDAC IVSNSTITRD DFYDGLYAEL
110 120 130 140 150
LNNPDVKELK QIPSKRSPHL SMIYLNIEFD LNFSRTAYTS LPDNLDILNE
160 170 180 190 200
NILKNMDELD TRAINGVRNT DIIDAFVPNH SEEAFRVMVR TIKLWTKKRG
210 220 230 240 250
IYGYVYCFLN GISIEILVAQ VISENYQLDN VRLLEKFFQV YSSWDWLRTP
260 270 280 290 300
VMLGTNDDFN DKKKEGVIQI LTPASPSENA AFSITKFSLE MIKRELKRGQ
310 320 330 340 350
EIVHEFSSEG VNDWAKLFKP RHLFCGYYIF IEFIVTSSSL EGLTTTIGKF
360 370 380 390 400
ESGLVNLMKG LSEIEEIIEA NVIPNGFLDE ENEKYFYYVG MNVKRDCPVD
410 420 430 440 450
ISTPLNSFLS IVNSGKDLIV DASIKKRSEI PTKFAHSVKR SITKSQEIKE
460 470 480 490 500
TSSQVPSSAI TETFDIPTKP SIEQQLKAKE ENSIPNEEKK EQLKKEMKQE
510 520
ANTIVKNSST DDDFMKRFTR KN
Length:522
Mass (Da):59,810
Last modified:June 7, 2005 - v1
Checksum:iB4A546672B639CBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS571148 Genomic DNA Translation: EAL50821.1
RefSeqiXP_656205.1, XM_651113.1

Genome annotation databases

GeneIDi3410521
KEGGiehi:EHI_012040

Similar proteinsi

Entry informationi

Entry nameiPAP_ENTHI
AccessioniPrimary (citable) accession number: Q51D88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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