Reviewed, UniProtKB/Swiss-Prot Q51879 (NIR_PHOLA)

Names and origin

Protein names

Recommended name:
    Ferredoxin--nitrite reductase
    EC=1.7.7.1

Gene names

Name:	nirA
Synonyms:	nir

Organism

Phormidium laminosum

Taxonomic identifier

32059 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Phormidium

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	NH3 + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-nitrite reductase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Ferredoxin--nitrite reductase		PRO_0000199956
Sites
	Metal binding	396	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	402	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	437	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	441	1	Iron (siroheme axial ligand) By similarity
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51879-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D4BB0816EABD9C80	FASTA	510	56,709
	10 20 30 40 50 60 MTSTVPAETS LNKFEKLKSE KDGLAVKSEL EDFARLGWEA MDETDRDHRL RWMGVFFRPV 70 80 90 100 110 120 SQGKFMLRMR IPNGILTSGQ IRVLAEVVER YGEDGNADIT TRPNLQLRGI RLEDIPDIFR 130 140 150 160 170 180 RFEQAGLTSI QSGMDNVRNI TGSPVAGIDA DELIDTRGLV RKVQDMITNN GEGNPSFSNL 190 200 210 220 230 240 PRKFNIAIAG CRDNSVHAEI NDIAFVPAYK DGKLGFNVLV GGFFSAKRCE AAVPLNAWVD 250 260 270 280 290 300 PRDVVALCEA ILIVYRITGC GANRQKSRLM WLIDEWGMDK FRAEVEQQLG HPLQTAAPKD 310 320 330 340 350 360 EILWDKRDHI GIHAQKKPGL NYVGLLVPVG RLYAPMFDLA RIAEVYGDGE MRLTVERKRD 370 380 390 400 410 420 HSRTCPMSSV ASLLKEPLLE KFSVSPGLLV RSLVSCTGAQ FCNFALIETK NRAMALIREL 430 440 450 460 470 480 ESELELARPV RIHWTGCPNS CGQPQVADIG LMGTKVRKDG KATEGVDLYM GGKVGKHAEL 490 500 510 GTCVQKGIPC DDLKPILRNL LIEHFGARPK

References

[1]

"Isolation, sequence and expression in Escherichia coli of the nitrite reductase gene from the filamentous, thermophilic cyanobacterium Phormidium laminosum." Merchan F., Prieto R., Kindle K.L., Llama M.J., Serra J.L., Fernandez E. Plant Mol. Biol. 27:1037-1042(1995) [PubMed: 7766873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OH-1-P-CL1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z19598 Genomic DNA. Translation: CAA79655.1.
PIR	S56640.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.7.1. 18650.
Family and domain databases
InterPro	IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_PHOLA

Accession

Primary (citable) accession number: Q51879

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)