Reviewed, UniProtKB/Swiss-Prot Q51876 (LEP_PHOLA)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Synonyms:	lep

Organism

Phormidium laminosum

Taxonomic identifier

32059 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Oscillatoriales › Phormidium

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Signal peptidase I		PRO_0000109512
Regions
	Topological domain	1 – 33	33	Cytoplasmic Potential
	Transmembrane	34 – 50	17	Potential
	Topological domain	51 – 203	153	Extracellular Potential
Sites
	Active site	59	1	By similarity
	Active site	109	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51876-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 887C4F9C93F5CD41	FASTA	203	22,485
	10 20 30 40 50 60 MSSESDSPTP QTPPAQPAAS QPKADSPLME GIKTIGLSVV LALGIRTFVA EARYIPSESM 70 80 90 100 110 120 LPTLEVNDRL IVEKISYHFN PPRRGDIIVF HPTEALKQQN PSLNEAFIKR VIGLPGETVQ 130 140 150 160 170 180 VTGGRVLING QPLEENYIQS PPDYQWGPEK VPADSFLVLG DNRNNSYDSH FWGYVPRQNI 190 200 IGRAVVRFWP VNRLGELGPP PSY

References

[1]	"Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum." Packer J.C.L., Andre D., Howe C.J. Plant Mol. Biol. 27:199-204(1995) [PubMed: 7865790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81990 Genomic DNA. Translation: CAA57518.1.
PIR	S51921.
3D structure databases
SMR	Q51876. Positions 46-196.
ModBase	Search...
Protein family/group databases
MEROPS	S26.008.
Enzyme and pathway databases
BRENDA	3.4.21.89. 18650.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_PHOLA

Accession

Primary (citable) accession number: Q51876

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)