Q51804 (PORA_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate synthase subunit porA
EC=1.2.7.1

Alternative name(s):
Pyruvate oxidoreductase alpha chain
Short name=POR
Pyruvic-ferredoxin oxidoreductase subunit alpha

Gene names

Name:	porA
Ordered Locus Names:	PF0966

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	pyruvate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Pyruvate synthase subunit porA		PRO_0000099900
Regions
	Compositional bias	69 – 72	4	Poly-Ala
Experimental info
	Sequence conflict	277	1	K → N in CAA59505. Ref.1
	Sequence conflict	355	1	L → H in CAA59505. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q51804 [UniParc]. Last modified April 3, 2002. Version 2. Checksum: 16204779F27B9BC3	FASTA	396	44,177
	10 20 30 40 50 60 MPIRKVMKAN EAAAWAAKLA KPKVIAAFPI TPSTLIPEKI SEFVANGELD AEFIKVESEH 70 80 90 100 110 120 SAISACVGAA AAGVRTFTAT ASQGLALMHE ILFIAAGMRL PIVMAIGNRA LSAPINIWND 130 140 150 160 170 180 WQDTISQRDT GWMQFYAENN QEALDLILIA YKVAEDERVL LPAMVGFDAF ILTHTVEPVE 190 200 210 220 230 240 IPDQEVVDEF LGEYEPKHAY IDPARPITQG SLAFPAHYME SRYTVWEAME RAKKVIDEAF 250 260 270 280 290 300 AEFEKKFGRK YQKIEEYKTE DADIIFVTMG SLAGTLKEWI DKKREEGYKV GAAKITVYRP 310 320 330 340 350 360 FPVEEIRELA KKAKVLAFLE KNITIGLYGA VFTDASAALI NESEKPLMVD FIVGLGGRDV 370 380 390 TFNQLDEALE IAEKALKEGK VENPINWIGL RWELVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima." Kletzin A., Adams M.W.W. J. Bacteriol. 178:248-257(1996) [PubMed: 8550425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 5-15. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima." Blamey J.M., Adams M.W.W. Biochemistry 33:1000-1007(1994) [PubMed: 8305426] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-32.
[4]	"Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus." Blamey J.M., Adams M.W.W. Biochim. Biophys. Acta 1161:19-27(1993) [PubMed: 8380721] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85250 Genomic DNA. Translation: CAA59505.1. AE009950 Genomic DNA. Translation: AAL81090.1.
PIR	T45088.
RefSeq	NP_578695.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q51804.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q51804. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000005763; EBPYRP00000005624; EBPYRG00000005762.
GeneID	1468831.
GenomeReviews	Gene locus PF0966 in contig AE009950_GR.
KEGG	pfu:PF0966.
NMPDR	fig|186497.1.peg.1002.
Phylogenomic databases
GeneTree	EBGT00050000022252.
HOGENOM	HBG313202.
OMA	EIEVWDG.
ProtClustDB	PRK08367.
Family and domain databases
InterPro	IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00169.
Pfam	PF01855. POR_N. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORA_PYRFU

Accession

Primary (citable) accession number: Q51804

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	April 3, 2002
Last modified:	November 16, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program