Reviewed, UniProtKB/Swiss-Prot Q51800 (VORD_PYRFU)

Names and origin

Protein names

Recommended name:
    Ketoisovalerate oxidoreductase subunit vorD
      Short name=VOR
    EC=1.2.7.7
Alternative name(s):
    2-oxoisovalerate oxidoreductase delta chain
    2-oxoisovalerate ferredoxin reductase subunit delta

Gene names

Name:	vorD
Ordered Locus Names:	PF0970

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Ketoisovalerate oxidoreductase subunit vorD		PRO_0000099962
Regions
	Domain	44 – 73	30	4Fe-4S ferredoxin-type 1
	Domain	74 – 103	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	83	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	86	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	93	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q51800-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F609078E069DB899	FASTA	105	11,851
	10 20 30 40 50 60 MNTLFGKTKE EAKPIVLKSV DEYPEAPISL GTTLVNPTGD WRTFKPVVNE EKCVKCYICW 70 80 90 100 KYCPEPAIYI KPDGYVAIDY DYCKGCGICA NECPTKAITM IKEEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima." Kletzin A., Adams M.W.W. J. Bacteriol. 178:248-257(1996) [PubMed: 8550425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85250 Genomic DNA. Translation: CAA59501.1. AE009950 Genomic DNA. Translation: AAL81094.1.
PIR	T45084.
RefSeq	NP_578699.1.
3D structure databases
SMR	Q51800. Positions 40-102.
ModBase	Search...
Genome annotation databases
GeneID	1468835.
GenomeReviews	Gene locus PF0970 in contig AE009950_GR.
KEGG	pfu:PF0970.
NMPDR	fig|186497.1.peg.1006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734156.
OMA	VDYEYCK.
Enzyme and pathway databases
BRENDA	1.2.7.7. 321.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR011898. PorD_KorD. [Graphical view]
TIGRFAMs	TIGR02179. PorD_KorD. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VORD_PYRFU

Accession

Primary (citable) accession number: Q51800

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)