Q51800 (VORD_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketoisovalerate oxidoreductase subunit vorD
Short name=VOR
EC=1.2.7.7

Alternative name(s):
2-oxoisovalerate ferredoxin reductase subunit delta
2-oxoisovalerate oxidoreductase delta chain

Gene names

Name:	vorD
Ordered Locus Names:	PF0970

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+.
Cofactor	Binds 2 4Fe-4S clusters By similarity.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.
Sequence similarities	Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity Inferred from electronic annotation. Source: EC 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	Ketoisovalerate oxidoreductase subunit vorD		PRO_0000099962
Regions
	Domain	44 – 73	30	4Fe-4S ferredoxin-type 1
	Domain	74 – 103	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) Potential
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	83	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	86	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	89	1	Iron-sulfur 2 (4Fe-4S) Potential
	Metal binding	93	1	Iron-sulfur 1 (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q51800 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F609078E069DB899	FASTA	105	11,851
	10 20 30 40 50 60 MNTLFGKTKE EAKPIVLKSV DEYPEAPISL GTTLVNPTGD WRTFKPVVNE EKCVKCYICW 70 80 90 100 KYCPEPAIYI KPDGYVAIDY DYCKGCGICA NECPTKAITM IKEEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima." Kletzin A., Adams M.W.W. J. Bacteriol. 178:248-257(1996) [PubMed: 8550425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85250 Genomic DNA. Translation: CAA59501.1. AE009950 Genomic DNA. Translation: AAL81094.1.
PIR	T45084.
RefSeq	NP_578699.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q51800.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000004961; EBPYRP00000004822; EBPYRG00000004960.
GeneID	1468835.
GenomeReviews	Gene locus PF0970 in contig AE009950_GR.
KEGG	pfu:PF0970.
NMPDR	fig|186497.1.peg.1006.
Phylogenomic databases
GeneTree	EBGT00050000022662.
HOGENOM	HBG734156.
OMA	KCYICWK.
ProtClustDB	PRK09623.
Family and domain databases
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR011898. PorD_KorD. [Graphical view]
KO	K00188.
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR02179. PorD_KorD. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VORD_PYRFU

Accession

Primary (citable) accession number: Q51800

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program