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Protein

Trans-2,3-dihydro-3-hydroxyanthranilate isomerase

Gene

phzF

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Isomerase that catalyzes the condensation of two molecules of trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine ring system. The final product is not yet known.2 Publications

Catalytic activityi

(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxyate = (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate.3 Publications

Pathwayi: phenazine biosynthesis

This protein is involved in the pathway phenazine biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway phenazine biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei451

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAntibiotic biosynthesis, Virulence

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13700.
BRENDAi5.3.3.17. 5121.
UniPathwayiUPA00099.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-2,3-dihydro-3-hydroxyanthranilate isomerase (EC:5.3.3.173 Publications)
Alternative name(s):
Phenazine/pyocyanine biosynthesis protein PhzF
Gene namesi
Name:phzF
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Chemistry databases

DrugBankiDB03644. 3-Hydroxyanthranilic Acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001623821 – 278Trans-2,3-dihydro-3-hydroxyanthranilate isomeraseAdd BLAST278

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Beta strandi16 – 23Combined sources8
Helixi26 – 28Combined sources3
Helixi31 – 41Combined sources11
Beta strandi46 – 50Combined sources5
Beta strandi53 – 63Combined sources11
Beta strandi68 – 70Combined sources3
Helixi73 – 84Combined sources12
Beta strandi90 – 96Combined sources7
Beta strandi99 – 108Combined sources10
Beta strandi111 – 118Combined sources8
Beta strandi123 – 126Combined sources4
Helixi130 – 137Combined sources8
Beta strandi147 – 159Combined sources13
Helixi163 – 168Combined sources6
Helixi173 – 176Combined sources4
Beta strandi183 – 190Combined sources8
Beta strandi193 – 200Combined sources8
Helixi201 – 203Combined sources3
Beta strandi205 – 207Combined sources3
Helixi212 – 224Combined sources13
Beta strandi234 – 238Combined sources5
Helixi240 – 242Combined sources3
Beta strandi246 – 255Combined sources10
Beta strandi258 – 276Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T6KX-ray1.80A1-278[»]
1U1VX-ray1.70A1-278[»]
1U1WX-ray1.35A/B1-278[»]
1U1XX-ray1.88A/B1-278[»]
1XUAX-ray1.90A/B1-278[»]
1XUBX-ray1.30A1-278[»]
5IWEX-ray1.71A1-278[»]
ProteinModelPortaliQ51792.
SMRiQ51792.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ51792.

Family & Domainsi

Sequence similaritiesi

Belongs to the PhzF family.Curated

Phylogenomic databases

KOiK06998.

Family and domain databases

InterProiView protein in InterPro
IPR003719. Phenazine_PhzF.
PANTHERiPTHR13774. PTHR13774. 1 hit.
PfamiView protein in Pfam
PF02567. PhzC-PhzF. 1 hit.
PIRSFiPIRSF016184. PhzC_PhzF. 1 hit.
TIGRFAMsiTIGR00654. PhzF_family. 1 hit.

Sequencei

Sequence statusi: Complete.

Q51792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNYVIIDAF ASVPLEGNPV AVFFDADDLP PAQMQRIARE MNLSESTFVL
60 70 80 90 100
KPRNGGDALI RIFTPVNELP FAGHPLLGTA IALGAHTDNH RLYLETQMGT
110 120 130 140 150
IAFELERQNG SVIAASMDQP IPTWTALGRD AELLKALGIS DSTFPIEIYH
160 170 180 190 200
NGPRHVFVGL PSIDALSALH PDHRALSNFH DMAINCFAGA GRRWRSRMFS
210 220 230 240 250
PAYGVVEDAA TGSAAGPLAI HLARHGQIEF GQPVEILQGV EIGRPSLMFA
260 270
KAEGRAEQLT RVEVSGNGVT FGRGTIVL
Length:278
Mass (Da):30,053
Last modified:November 1, 1996 - v1
Checksum:i345086CE9679224D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L48616 Genomic DNA. Translation: AAC18905.1.
RefSeqiWP_043050175.1. NZ_JXCQ01000041.1.

Genome annotation databases

KEGGiag:AAC18905.

Similar proteinsi

Entry informationi

Entry nameiPHZF_PSEFL
AccessioniPrimary (citable) accession number: Q51792
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families