Reviewed, UniProtKB/Swiss-Prot Q51791 (PHZE_PSEFL)

Names and origin

Protein names

Recommended name:
    Anthranilate synthase, phenazine specific
    EC=4.1.3.27
Including the following 1 domains:
    1- Recommended name:
            Glutamine amidotransferase

Gene names

Name:

phzE

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Involved in the biosynthesis of the antibiotic, phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control.
Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Antibiotic biosynthesis; phenazine biosynthesis.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis Virulence
Domain	Glutamine amidotransferase
Molecular function	Lyase Transferase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Anthranilate synthase, phenazine specific		PRO_0000056908
Regions
	Domain	437 – 628	192	Glutamine amidotransferase type-1
	Region	1 – 434	434	Anthranilate synthase component I
Sites
	Active site	517	1	For GATase activity By similarity
	Active site	602	1	For GATase activity By similarity
	Active site	604	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51791-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D65235CA6139E0FE	FASTA	637	69,873
	10 20 30 40 50 60 MSQAAARLME RILQPVPEPF ALLYRPESSG PGLLNVLIGE MSQPQVLADI DLPAPSIGAP 70 80 90 100 110 120 RLDVLTLIPY CQIAERGFAA VDDQSPLLAM NITEQQTISI EQMLALLPNV PIQLNNERFD 130 140 150 160 170 180 LSDASYAEIV SQVIANEIGS GEGANFVIKR TFLAEISEYQ PASALSFFRH LLEREKGVYW 190 200 210 220 230 240 TFIIHTGSRT FVGASPERHI SVKDGLAVMN PISGTYRYPP AGPSLTEVMD FLADRKEADE 250 260 270 280 290 300 LYMVVDEELK MMARICEDGG HVLGPYLKEM THLAHTEYFI EGRTRRDVRE ILHETLFAPT 310 320 330 340 350 360 VTGSPLESAC RVIERYEPQG RAYYSGMAAL IGSDGKGGRS LDSAILIRTA DIDNCGQVRI 370 380 390 400 410 420 SVGSTIVRHS EPLTEAAESR AKAAGLIAAL KNQAASRFGD HLQVRAALAS RNAYVSDFWL 430 440 450 460 470 480 MNSQQRQQTQ SDFSGRQVLI VDAEDTFTSM IAKQLRALGL VVTVRSFSDE YSFDGYDLVI 490 500 510 520 530 540 MGPGPGNPSD VQLPKIDHLH VAIRSLLNQQ RPFLAVCLSH QVLSLCLGLE LQRKAIPNQG 550 560 570 580 590 600 VQKQIDLFGN AERVGFYNTF AARSSSDRLD IDGIGTVEIS RDSETGEVHA LRGPAFASMQ 610 620 630 FHAESLLTQE GPRIIADLLR HALIHTPVDS SVSAAGR

References

[1]

"A seven-gene locus for synthesis of phenazine-1-carboxylic acid by Pseudomonas fluorescens 2-79." Mavrodi D.V., Ksenzenko V.N., Bonsall R.F., Cook R.J., Boronin A.M., Thomashow L.S. J. Bacteriol. 180:2541-2548(1998) [PubMed: 9573209] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL B-15132 / 2-79.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L48616 Genomic DNA. Translation: AAC18904.1.
3D structure databases
SMR	Q51791. Positions 117-391, 351-491, 437-622.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13690.
BRENDA	4.1.3.27. 329.
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR015890. Chorismate-bd_C. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
PANTHER	PTHR11236. TRPE_1_chor_bd. 1 hit.
Pfam	PF00425. Chorismate_bind. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHZE_PSEFL

Accession

Primary (citable) accession number: Q51791

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)