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Protein
Submitted name:

Iron-sulfur protein large subunit of cumene dioxygenase

Gene

cumA1

Organism
Pseudomonas fluorescens
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron-sulfur (2Fe-2S)Combined sources
Metal bindingi103 – 1031Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources
Metal bindingi121 – 1211Iron-sulfur (2Fe-2S)Combined sources
Metal bindingi124 – 1241Iron-sulfur (2Fe-2S); via pros nitrogenCombined sources
Metal bindingi126 – 1261Iron-sulfur (2Fe-2S)Combined sources
Metal bindingi234 – 2341Iron; via tele nitrogenCombined sources
Metal bindingi240 – 2401Iron; via tele nitrogenCombined sources
Metal bindingi388 – 3881IronCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DioxygenaseImported, Oxidoreductase

Keywords - Ligandi

2Fe-2SUniRule annotationCombined sources, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Submitted name:
Iron-sulfur protein large subunit of cumene dioxygenaseImported
Gene namesi
Name:cumA1Imported
OrganismiPseudomonas fluorescensImported
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi145 ↔ 152Combined sources

Interactioni

Protein-protein interaction databases

IntActiQ51743. 1 interaction.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WQLX-ray2.20A1-459[»]
ProteinModelPortaliQ51743.
SMRiQ51743. Positions 19-459.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ51743.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 14183RieskeInterPro annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.UniRule annotation

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q51743-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSIINKEVQ EAPLKWVKNW SDEEIKALVD EEKGLLDPRI FSDQDLYEIE
60 70 80 90 100
LERVFARSWL LLGHEGHIPK AGDYLTTYMG EDPVIVVRQK DRSIKVFLNQ
110 120 130 140 150
CRHRGMRIER SDFGNAKSFT CTYHGWAYDT AGNLVNVPYE KEAFCDKKEG
160 170 180 190 200
DCGFDKADWG PLQARVDTYK GLIFANWDTE APDLKTYLSD ATPYMDVMLD
210 220 230 240 250
RTEAVTQVIT GMQKTVIPCN WKFAAEQFCS DMYHAGTMAH LSGVLSSLPP
260 270 280 290 300
EMDLSQVKLP SSGNQFRAKW GGHGTGWFND DFALLQAIMG PKVVDYWTKG
310 320 330 340 350
PAAERAKERL GKVLPADRMV AQHMTIFPTC SFLPGINTVR TWHPRGPNEI
360 370 380 390 400
EVWSFIVVDA DAPEDIKEEY RRKNIFTFNQ GGTYEQDDGE NWVEVQRGLR
410 420 430 440 450
GYKARSRPLC AQMGAGVPNK NNPEFPGKTS YVYSEEAARG FYHHWSRMMS

EPSWDTLKS
Length:459
Mass (Da):52,293
Last modified:November 1, 1996 - v1
Checksum:iBA940BD0092D26BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37828 Genomic DNA. Translation: BAA07074.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37828 Genomic DNA. Translation: BAA07074.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WQLX-ray2.20A1-459[»]
ProteinModelPortaliQ51743.
SMRiQ51743. Positions 19-459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ51743. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ51743.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and nucleotide sequence of the genes involved in the meta-cleavage pathway of cumene degradation in Pseudomonas fluorescens IP01."
    Habe H., Kimura T., Nojiri H., Yamane H., Omori T.
    J. Ferment. Bioeng. 81:247-254(1996)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IP01Imported.
  2. "Crystal structure of the terminal oxygenase component of cumene dioxygenase from Pseudomonas fluorescens IP01."
    Dong X., Fushinobu S., Fukuda E., Terada T., Nakamura S., Shimizu K., Nojiri H., Omori T., Shoun H., Wakagi T.
    J. Bacteriol. 187:2483-2490(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH IRON AND IRON-SULFUR (2FE-2S), DISULFIDE BONDS.

Entry informationi

Entry nameiQ51743_PSEFL
AccessioniPrimary (citable) accession number: Q51743
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: March 16, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.