Iron-sulfur protein large subunit of cumene dioxygenase

Preferred order of sections

Names and origin

Protein names

Submitted name:
Iron-sulfur protein large subunit of cumene dioxygenase EMBL BAA07074.1

Gene names

Name:

cumA1 EMBL BAA07074.1

Organism

Pseudomonas fluorescens EMBL BAA07074.1

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 1 Rieske domain. RuleBase RU004493

Ontologies

Keywords
Ligand	2Fe-2S RuleBase RU004492 PDB 1WQL Iron Iron-sulfur Metal-binding
Molecular function	Dioxygenase EMBL BAA07074.1 Oxidoreductase
Technical term	3D-structure PDB 1WQL
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of two atoms of oxygen into one donor Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

101

1

Iron-sulfur (2Fe-2S) PDB 1WQL

Metal binding

103

1

Iron-sulfur (2Fe-2S); via pros nitrogen PDB 1WQL

Metal binding

121

1

Iron-sulfur (2Fe-2S) PDB 1WQL

Metal binding

124

1

Iron-sulfur (2Fe-2S); via pros nitrogen PDB 1WQL

Metal binding

126

1

Iron-sulfur (2Fe-2S) PDB 1WQL

Metal binding

234

1

Iron; via tele nitrogen PDB 1WQL

Metal binding

240

1

Iron; via tele nitrogen PDB 1WQL

Metal binding

388

1

Iron PDB 1WQL

Sequences

Sequence

Length

Mass (Da)

Tools

Q51743 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BA940BD0092D26BA
Show »

FASTA

459

52,293

        10         20         30         40         50         60 
MSSIINKEVQ EAPLKWVKNW SDEEIKALVD EEKGLLDPRI FSDQDLYEIE LERVFARSWL 

        70         80         90        100        110        120 
LLGHEGHIPK AGDYLTTYMG EDPVIVVRQK DRSIKVFLNQ CRHRGMRIER SDFGNAKSFT 

       130        140        150        160        170        180 
CTYHGWAYDT AGNLVNVPYE KEAFCDKKEG DCGFDKADWG PLQARVDTYK GLIFANWDTE 

       190        200        210        220        230        240 
APDLKTYLSD ATPYMDVMLD RTEAVTQVIT GMQKTVIPCN WKFAAEQFCS DMYHAGTMAH 

       250        260        270        280        290        300 
LSGVLSSLPP EMDLSQVKLP SSGNQFRAKW GGHGTGWFND DFALLQAIMG PKVVDYWTKG 

       310        320        330        340        350        360 
PAAERAKERL GKVLPADRMV AQHMTIFPTC SFLPGINTVR TWHPRGPNEI EVWSFIVVDA 

       370        380        390        400        410        420 
DAPEDIKEEY RRKNIFTFNQ GGTYEQDDGE NWVEVQRGLR GYKARSRPLC AQMGAGVPNK 

       430        440        450 
NNPEFPGKTS YVYSEEAARG FYHHWSRMMS EPSWDTLKS

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References

[1]	"Cloning and nucleotide sequence of the genes involved in the meta-cleavage pathway of cumene degradation in Pseudomonas fluorescens IP01." Habe H., Kimura T., Nojiri H., Yamane H., Omori T. J. Ferment. Bioeng. 81:247-254(1996) Cited for: NUCLEOTIDE SEQUENCE. Strain: IP01 EMBL BAA07074.1.
[2]	Aoki H., Kimura T., Habe H., Nojiri H., Yamane H., Kodama T., Omori T. Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: IP01 EMBL BAA07074.1.
[3]	"Crystal structure of the terminal oxygenase component of cumene dioxygenase from Pseudomonas fluorescens IP01." Dong X., Fushinobu S., Fukuda E., Terada T., Nakamura S., Shimizu K., Nojiri H., Omori T., Shoun H., Wakagi T. J. Bacteriol. 187:2483-2490(2005) [PubMed: 15774891] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH IRON AND IRON-SULFUR (2FE-2S).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D37828 Genomic DNA. Translation: BAA07074.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WQL	X-ray	2.20	A	1-459	[»]

ProteinModelPortal

Q51743.

SMR

Q51743. Positions 19-459.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q51743. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]

PRINTS

PR00090. RNGDIOXGNASE.

SUPFAM

SSF50022. Rieske_dom. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q51743_PSEFL

Accession

Primary (citable) accession number: Q51743

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)