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Protein

Tungsten-containing aldehyde ferredoxin oxidoreductase

Gene

aor

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of aldehydes to their corresponding carboxylic acids. May have a pyroglycolytic (saccharolytic) role.

Catalytic activityi

An aldehyde + H2O + 2 oxidized ferredoxin = a carboxylate + 2 H+ + 2 reduced ferredoxin.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Inhibited by arsenite, iodoacetate and cyanide.

Kineticsi

Significant activity only with aliphatic and aromatic aldehydes and only at low concentration (0.5 mM).

  1. KM=0.72 mM for formaldehyde1 Publication
  1. Vmax=295 µmol/min/mg enzyme with formaldehyde as substrate1 Publication

pH dependencei

Optimum pH is above 10.1 Publication

Temperature dependencei

Optimum temperature is above 90 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881Iron-sulfur (4Fe-4S)
Metal bindingi291 – 2911Iron-sulfur (4Fe-4S)
Metal bindingi295 – 2951Iron-sulfur (4Fe-4S)
Metal bindingi494 – 4941Iron-sulfur (4Fe-4S)

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Tungsten

Enzyme and pathway databases

BRENDAi1.2.7.5. 5243.
SABIO-RKQ51739.

Names & Taxonomyi

Protein namesi
Recommended name:
Tungsten-containing aldehyde ferredoxin oxidoreductase (EC:1.2.7.5)
Gene namesi
Name:aor
Ordered Locus Names:PF0346
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Tungsten-containing aldehyde ferredoxin oxidoreductasePRO_0000064606Add
BLAST

Proteomic databases

PRIDEiQ51739.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0346.

Structurei

Secondary structure

1
605
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Turni13 – 164Combined sources
Beta strandi17 – 226Combined sources
Helixi25 – 317Combined sources
Helixi35 – 4511Combined sources
Beta strandi59 – 635Combined sources
Turni65 – 684Combined sources
Beta strandi69 – 713Combined sources
Beta strandi77 – 826Combined sources
Turni84 – 863Combined sources
Beta strandi87 – 948Combined sources
Helixi98 – 1047Combined sources
Beta strandi108 – 1147Combined sources
Beta strandi120 – 1256Combined sources
Beta strandi128 – 1336Combined sources
Turni135 – 1395Combined sources
Helixi142 – 15312Combined sources
Beta strandi159 – 1624Combined sources
Helixi165 – 1684Combined sources
Beta strandi176 – 1783Combined sources
Turni179 – 1813Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi186 – 1883Combined sources
Helixi189 – 1957Combined sources
Beta strandi198 – 2047Combined sources
Helixi214 – 23017Combined sources
Helixi232 – 2354Combined sources
Helixi237 – 2415Combined sources
Helixi243 – 2453Combined sources
Helixi246 – 2527Combined sources
Turni259 – 2624Combined sources
Helixi269 – 2724Combined sources
Helixi274 – 2807Combined sources
Beta strandi282 – 2865Combined sources
Beta strandi296 – 3016Combined sources
Turni302 – 3043Combined sources
Beta strandi305 – 3084Combined sources
Helixi312 – 3187Combined sources
Helixi320 – 3223Combined sources
Helixi327 – 34014Combined sources
Helixi344 – 35916Combined sources
Helixi365 – 3684Combined sources
Helixi380 – 39011Combined sources
Helixi396 – 3994Combined sources
Helixi403 – 4097Combined sources
Helixi413 – 4153Combined sources
Helixi429 – 4313Combined sources
Helixi433 – 4419Combined sources
Helixi449 – 4513Combined sources
Helixi454 – 4574Combined sources
Beta strandi461 – 4633Combined sources
Helixi472 – 49120Combined sources
Helixi495 – 4984Combined sources
Helixi503 – 51412Combined sources
Helixi520 – 54122Combined sources
Helixi545 – 5484Combined sources
Helixi553 – 5575Combined sources
Turni564 – 5674Combined sources
Helixi572 – 58312Combined sources
Helixi593 – 5997Combined sources
Helixi602 – 6043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AORX-ray2.30A/B1-605[»]
ProteinModelPortaliQ51739.
SMRiQ51739. Positions 1-605.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ51739.

Family & Domainsi

Sequence similaritiesi

Belongs to the AOR/FOR family.Curated

Phylogenomic databases

eggNOGiarCOG00706. Archaea.
COG2414. LUCA.
HOGENOMiHOG000014651.
KOiK03738.
OMAiERIWNAE.

Family and domain databases

Gene3Di1.10.599.10. 1 hit.
3.60.9.10. 1 hit.
InterProiIPR013985. Ald_Fedxn_OxRdtase_3.
IPR013983. Ald_Fedxn_OxRdtase_N.
IPR001203. OxRdtase_Ald_Fedxn_C.
[Graphical view]
PfamiPF01314. AFOR_C. 1 hit.
PF02730. AFOR_N. 1 hit.
[Graphical view]
SMARTiSM00790. AFOR_N. 1 hit.
[Graphical view]
SUPFAMiSSF48310. SSF48310. 1 hit.
SSF56228. SSF56228. 1 hit.

Sequencei

Sequence statusi: Complete.

Q51739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGNWGRFIR VNLSTGDIKV EEYDEELAKK WLGSRGLAIY LLLKEMDPTV
60 70 80 90 100
DPLSPENKLI IAAGPLTGTS APTGGRYNVV TKSPLTGFIT MANSGGYFGA
110 120 130 140 150
ELKFAGYDAI VVEGKAEKPV YIYIKDEHIE IRDASHIWGK KVSETEATIR
160 170 180 190 200
KEVGSEKVKI ASIGPAGENL VKFAAIMNDG HRAAGRGGVG AVMGSKNLKA
210 220 230 240 250
IAVEGSKTVP IADKQKFMLV VREKVNKLRN DPVAGGGLPK YGTAVLVNII
260 270 280 290 300
NENGLYPVKN FQTGVYPYAY EQSGEAMAAK YLVRNKPCYA CPIGCGRVNR
310 320 330 340 350
LPTVGETEGP EYESVWALGA NLGINDLASI IEANHMCDEL GLDTISTGGT
360 370 380 390 400
LATAMELYEK GHIKDEELGD APPFRWGNTE VLHYYIEKIA KREGFGDKLA
410 420 430 440 450
EGSYRLAESY GHPELSMTVK KLELPAYDPR GAEGHGLGYA TNNRGGCHIK
460 470 480 490 500
NYMISPEILG YPYKMDPHDV SDDKIKMLIL FQDLTALIDS AGLCLFTTFG
510 520 530 540 550
LGADDYRDLL NAALGWDFTT EDYLKIGERI WNAERLFNLK AGLDPARDDT
560 570 580 590 600
LPKRFLEEPM PEGPNKGHTV RLKEMLPRYY KLRGWTEDGK IPKEKLEELG

IAEFY
Length:605
Mass (Da):66,631
Last modified:November 1, 1996 - v1
Checksum:i86254EDF6756C00D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79777 Genomic DNA. Translation: CAA56170.1.
AE009950 Genomic DNA. Translation: AAL80470.1.
RefSeqiWP_011011461.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80470; AAL80470; PF0346.
GeneIDi1468181.
KEGGipfu:PF0346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79777 Genomic DNA. Translation: CAA56170.1.
AE009950 Genomic DNA. Translation: AAL80470.1.
RefSeqiWP_011011461.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AORX-ray2.30A/B1-605[»]
ProteinModelPortaliQ51739.
SMRiQ51739. Positions 1-605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0346.

Proteomic databases

PRIDEiQ51739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80470; AAL80470; PF0346.
GeneIDi1468181.
KEGGipfu:PF0346.

Phylogenomic databases

eggNOGiarCOG00706. Archaea.
COG2414. LUCA.
HOGENOMiHOG000014651.
KOiK03738.
OMAiERIWNAE.

Enzyme and pathway databases

BRENDAi1.2.7.5. 5243.
SABIO-RKQ51739.

Miscellaneous databases

EvolutionaryTraceiQ51739.

Family and domain databases

Gene3Di1.10.599.10. 1 hit.
3.60.9.10. 1 hit.
InterProiIPR013985. Ald_Fedxn_OxRdtase_3.
IPR013983. Ald_Fedxn_OxRdtase_N.
IPR001203. OxRdtase_Ald_Fedxn_C.
[Graphical view]
PfamiPF01314. AFOR_C. 1 hit.
PF02730. AFOR_N. 1 hit.
[Graphical view]
SMARTiSM00790. AFOR_N. 1 hit.
[Graphical view]
SUPFAMiSSF48310. SSF48310. 1 hit.
SSF56228. SSF56228. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAOR_PYRFU
AccessioniPrimary (citable) accession number: Q51739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A significant amount of formaldehyde ferredoxin oxidoreductase activity has been observed.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.