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Protein

Tungsten-containing aldehyde ferredoxin oxidoreductase

Gene

aor

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of aldehydes to their corresponding carboxylic acids. May have a pyroglycolytic (saccharolytic) role.

Catalytic activityi

An aldehyde + H2O + 2 oxidized ferredoxin = a carboxylate + 2 H+ + 2 reduced ferredoxin.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Inhibited by arsenite, iodoacetate and cyanide.

Kineticsi

Significant activity only with aliphatic and aromatic aldehydes and only at low concentration (0.5 mM).

  1. KM=0.72 mM for formaldehyde1 Publication
  1. Vmax=295 µmol/min/mg enzyme with formaldehyde as substrate1 Publication

pH dependencei

Optimum pH is above 10.1 Publication

Temperature dependencei

Optimum temperature is above 90 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288Iron-sulfur (4Fe-4S)1
Metal bindingi291Iron-sulfur (4Fe-4S)1
Metal bindingi295Iron-sulfur (4Fe-4S)1
Metal bindingi494Iron-sulfur (4Fe-4S)1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Tungsten

Enzyme and pathway databases

BRENDAi1.2.7.5. 5243.
SABIO-RKQ51739.

Names & Taxonomyi

Protein namesi
Recommended name:
Tungsten-containing aldehyde ferredoxin oxidoreductase (EC:1.2.7.5)
Gene namesi
Name:aor
Ordered Locus Names:PF0346
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000646061 – 605Tungsten-containing aldehyde ferredoxin oxidoreductaseAdd BLAST605

Proteomic databases

PRIDEiQ51739.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0346.

Structurei

Secondary structure

1605
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Turni13 – 16Combined sources4
Beta strandi17 – 22Combined sources6
Helixi25 – 31Combined sources7
Helixi35 – 45Combined sources11
Beta strandi59 – 63Combined sources5
Turni65 – 68Combined sources4
Beta strandi69 – 71Combined sources3
Beta strandi77 – 82Combined sources6
Turni84 – 86Combined sources3
Beta strandi87 – 94Combined sources8
Helixi98 – 104Combined sources7
Beta strandi108 – 114Combined sources7
Beta strandi120 – 125Combined sources6
Beta strandi128 – 133Combined sources6
Turni135 – 139Combined sources5
Helixi142 – 153Combined sources12
Beta strandi159 – 162Combined sources4
Helixi165 – 168Combined sources4
Beta strandi176 – 178Combined sources3
Turni179 – 181Combined sources3
Beta strandi182 – 184Combined sources3
Beta strandi186 – 188Combined sources3
Helixi189 – 195Combined sources7
Beta strandi198 – 204Combined sources7
Helixi214 – 230Combined sources17
Helixi232 – 235Combined sources4
Helixi237 – 241Combined sources5
Helixi243 – 245Combined sources3
Helixi246 – 252Combined sources7
Turni259 – 262Combined sources4
Helixi269 – 272Combined sources4
Helixi274 – 280Combined sources7
Beta strandi282 – 286Combined sources5
Beta strandi296 – 301Combined sources6
Turni302 – 304Combined sources3
Beta strandi305 – 308Combined sources4
Helixi312 – 318Combined sources7
Helixi320 – 322Combined sources3
Helixi327 – 340Combined sources14
Helixi344 – 359Combined sources16
Helixi365 – 368Combined sources4
Helixi380 – 390Combined sources11
Helixi396 – 399Combined sources4
Helixi403 – 409Combined sources7
Helixi413 – 415Combined sources3
Helixi429 – 431Combined sources3
Helixi433 – 441Combined sources9
Helixi449 – 451Combined sources3
Helixi454 – 457Combined sources4
Beta strandi461 – 463Combined sources3
Helixi472 – 491Combined sources20
Helixi495 – 498Combined sources4
Helixi503 – 514Combined sources12
Helixi520 – 541Combined sources22
Helixi545 – 548Combined sources4
Helixi553 – 557Combined sources5
Turni564 – 567Combined sources4
Helixi572 – 583Combined sources12
Helixi593 – 599Combined sources7
Helixi602 – 604Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AORX-ray2.30A/B1-605[»]
ProteinModelPortaliQ51739.
SMRiQ51739.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ51739.

Family & Domainsi

Sequence similaritiesi

Belongs to the AOR/FOR family.Curated

Phylogenomic databases

eggNOGiarCOG00706. Archaea.
COG2414. LUCA.
HOGENOMiHOG000014651.
KOiK03738.
OMAiERIWNAE.

Family and domain databases

Gene3Di1.10.599.10. 1 hit.
3.60.9.10. 1 hit.
InterProiIPR013985. Ald_Fedxn_OxRdtase_3.
IPR013983. Ald_Fedxn_OxRdtase_N.
IPR001203. OxRdtase_Ald_Fedxn_C.
[Graphical view]
PfamiPF01314. AFOR_C. 1 hit.
PF02730. AFOR_N. 1 hit.
[Graphical view]
SMARTiSM00790. AFOR_N. 1 hit.
[Graphical view]
SUPFAMiSSF48310. SSF48310. 1 hit.
SSF56228. SSF56228. 1 hit.

Sequencei

Sequence statusi: Complete.

Q51739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGNWGRFIR VNLSTGDIKV EEYDEELAKK WLGSRGLAIY LLLKEMDPTV
60 70 80 90 100
DPLSPENKLI IAAGPLTGTS APTGGRYNVV TKSPLTGFIT MANSGGYFGA
110 120 130 140 150
ELKFAGYDAI VVEGKAEKPV YIYIKDEHIE IRDASHIWGK KVSETEATIR
160 170 180 190 200
KEVGSEKVKI ASIGPAGENL VKFAAIMNDG HRAAGRGGVG AVMGSKNLKA
210 220 230 240 250
IAVEGSKTVP IADKQKFMLV VREKVNKLRN DPVAGGGLPK YGTAVLVNII
260 270 280 290 300
NENGLYPVKN FQTGVYPYAY EQSGEAMAAK YLVRNKPCYA CPIGCGRVNR
310 320 330 340 350
LPTVGETEGP EYESVWALGA NLGINDLASI IEANHMCDEL GLDTISTGGT
360 370 380 390 400
LATAMELYEK GHIKDEELGD APPFRWGNTE VLHYYIEKIA KREGFGDKLA
410 420 430 440 450
EGSYRLAESY GHPELSMTVK KLELPAYDPR GAEGHGLGYA TNNRGGCHIK
460 470 480 490 500
NYMISPEILG YPYKMDPHDV SDDKIKMLIL FQDLTALIDS AGLCLFTTFG
510 520 530 540 550
LGADDYRDLL NAALGWDFTT EDYLKIGERI WNAERLFNLK AGLDPARDDT
560 570 580 590 600
LPKRFLEEPM PEGPNKGHTV RLKEMLPRYY KLRGWTEDGK IPKEKLEELG

IAEFY
Length:605
Mass (Da):66,631
Last modified:November 1, 1996 - v1
Checksum:i86254EDF6756C00D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79777 Genomic DNA. Translation: CAA56170.1.
AE009950 Genomic DNA. Translation: AAL80470.1.
RefSeqiWP_011011461.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80470; AAL80470; PF0346.
GeneIDi1468181.
KEGGipfu:PF0346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79777 Genomic DNA. Translation: CAA56170.1.
AE009950 Genomic DNA. Translation: AAL80470.1.
RefSeqiWP_011011461.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AORX-ray2.30A/B1-605[»]
ProteinModelPortaliQ51739.
SMRiQ51739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0346.

Proteomic databases

PRIDEiQ51739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80470; AAL80470; PF0346.
GeneIDi1468181.
KEGGipfu:PF0346.

Phylogenomic databases

eggNOGiarCOG00706. Archaea.
COG2414. LUCA.
HOGENOMiHOG000014651.
KOiK03738.
OMAiERIWNAE.

Enzyme and pathway databases

BRENDAi1.2.7.5. 5243.
SABIO-RKQ51739.

Miscellaneous databases

EvolutionaryTraceiQ51739.

Family and domain databases

Gene3Di1.10.599.10. 1 hit.
3.60.9.10. 1 hit.
InterProiIPR013985. Ald_Fedxn_OxRdtase_3.
IPR013983. Ald_Fedxn_OxRdtase_N.
IPR001203. OxRdtase_Ald_Fedxn_C.
[Graphical view]
PfamiPF01314. AFOR_C. 1 hit.
PF02730. AFOR_N. 1 hit.
[Graphical view]
SMARTiSM00790. AFOR_N. 1 hit.
[Graphical view]
SUPFAMiSSF48310. SSF48310. 1 hit.
SSF56228. SSF56228. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAOR_PYRFU
AccessioniPrimary (citable) accession number: Q51739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A significant amount of formaldehyde ferredoxin oxidoreductase activity has been observed.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.