Q51732 (PFPI_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intracellular protease 1
EC=3.2.-.-

Alternative name(s):
Intracellular protease I

Gene names

Name:	pfpI
Ordered Locus Names:	PF1719

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus.
Subunit structure	Homooligomer. Two active complexes, a 86 and a 66 kDa, have been found. They probably contain four and three subunits respectively.
Subcellular location	Cytoplasm Ref.1.
Miscellaneous	Is resistant to several denaturants.
Sequence similarities	Belongs to the peptidase C56 family. Contains 1 PfpI endopeptidase domain.
Biophysicochemical properties	Temperature dependence: Highly thermostable (a half-life of over 30 hours at 98 degrees Celsius). Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, acting on glycosyl bonds Inferred from electronic annotation. Source: InterPro peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	Intracellular protease 1		PRO_0000157826
Regions
	Domain	1 – 166	166	PfpI endopeptidase
Sites
	Active site	100	1	Nucleophile By similarity
	Active site	101	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q51732 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B4AA6FFB83FBE52F	FASTA	166	18,791
	10 20 30 40 50 60 MKILFLSANE FEDVELIYPY HRLKEEGHEV YIASFEKGVI TGKHGYSVKV DLTFDEVNPD 70 80 90 100 110 120 EFDALVLPGG RAPERVRLNE KAVEIARKMF TEGKPVATIC HGPQILISAG VLKGRKGTSY 130 140 150 160 IGIRDDMINA GVEWIDREVV VDGNWVSSRH PGDLYAWMRE FVKLLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus." Halio S.B., Blumentals I.I., Short S.A., Merrill B.M., Kelly R.M. J. Bacteriol. 178:2605-2612(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-28; 128-157 AND 159-166, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57642 Genomic DNA. Translation: AAB04694.1. AE009950 Genomic DNA. Translation: AAL81843.1.
PIR	JC6003.
RefSeq	NP_579448.1. NC_003413.1.
3D structure databases
ProteinModelPortal	Q51732.
SMR	Q51732. Positions 1-166.
ModBase	Search...
Protein-protein interaction databases
STRING	186497.PF1719.
Protein family/group databases
MEROPS	C56.001.
Proteomic databases
PRIDE	Q51732.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL81843; AAL81843; PF1719.
GeneID	1469596.
KEGG	pfu:PF1719.
Phylogenomic databases
eggNOG	COG0693.
HOGENOM	HOG000063195.
KO	K05520.
OMA	GRKATCW.
ProtClustDB	CLSK253649.
Family and domain databases
InterPro	IPR006286. Peptidase_C56. IPR002818. ThiJ/PfpI. [Graphical view]
Pfam	PF01965. DJ-1_PfpI. 1 hit. [Graphical view]
TIGRFAMs	TIGR01382. PfpI. 1 hit.
PROSITE	PS51276. PEPTIDASE_C56_PFPI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFPI_PYRFU

Accession

Primary (citable) accession number: Q51732

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program