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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

nirE

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin (By similarity). Inactivation of uroporphyrinogen-III methyltransferase results in the loss of nitrite and nitric oxide reductase activities, but not of nitrous oxide reductase activity. Likely involved in heme D1 biosynthesis.By similarity

Catalytic activityi

2 S-adenosyl-L-methionine + uroporphyrinogen III = 2 S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi: siroheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.
Proteins known to be involved in this subpathway in this organism are:
  1. Uroporphyrinogen-III C-methyltransferase (nirE)
This subpathway is part of the pathway siroheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei146S-adenosyl-L-methionineBy similarity1
Binding sitei199S-adenosyl-L-methionine; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processPorphyrin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPDEN318586:G1GW1-2515-MONOMER
UniPathwayiUPA00262; UER00211

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:nirE
Ordered Locus Names:Pden_2488
OrganismiParacoccus denitrificans (strain Pd 1222)
Taxonomic identifieri318586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
Proteomesi
  • UP000000361 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001503851 – 287Uroporphyrinogen-III C-methyltransferaseAdd BLAST287

Proteomic databases

PRIDEiQ51701

Interactioni

Protein-protein interaction databases

STRINGi318586.Pden_2488

Structurei

3D structure databases

ProteinModelPortaliQ51701
SMRiQ51701
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 118S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105CB8 Bacteria
COG0007 LUCA
HOGENOMiHOG000290519
KOiK02303
OMAiYMGIHNL
OrthoDBiPOG091H0526

Family and domain databases

CDDicd11642 SUMT, 1 hit
Gene3Di3.30.950.10, 1 hit
3.40.1010.10, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR014776 4pyrrole_Mease_sub2
IPR006366 CobA/CysG_C
IPR003043 Uropor_MeTrfase_CS
PfamiView protein in Pfam
PF00590 TP_methylase, 1 hit
SUPFAMiSSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR01469 cobA_cysG_Cterm, 1 hit
PROSITEiView protein in PROSITE
PS00839 SUMT_1, 1 hit
PS00840 SUMT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q51701-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKTVTNGA AQGKAARSGA DGAVRGKAGM GRVDLIGAGP GDPELLTLRA
60 70 80 90 100
LRLLQQADVV VHDRLVSDEV MACIPAHVRR IPVGKAAGFH PVPQEQINAL
110 120 130 140 150
LVELGLSGLT VARLKGGDPT IFGRGGEEFE AVTRAGIPCD YVPGITAAQG
160 170 180 190 200
AAVSARFPLT HRGLATGLRH VTGHRARDAA LDLDWASLAD PQTTLAIYMG
210 220 230 240 250
AANMAEIARE LIRHGMPADL PVLAVSQAST PQEQRLHATL QDIAAALARK
260 270 280
PLPAPVLFIV GHVAAMAEDC ALPQELYRPE WRLVAHG
Length:287
Mass (Da):30,029
Last modified:November 1, 1996 - v1
Checksum:iAA319E29BAC6C461
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05002 Genomic DNA Translation: AAA93119.1
CP000489 Genomic DNA Translation: ABL70575.1
RefSeqiWP_011748768.1, NC_008686.1

Genome annotation databases

EnsemblBacteriaiABL70575; ABL70575; Pden_2488
KEGGipde:Pden_2488

Similar proteinsi

Entry informationi

Entry nameiNIRE_PARDP
AccessioniPrimary (citable) accession number: Q51701
Secondary accession number(s): A1B4Y1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health