Reviewed, UniProtKB/Swiss-Prot Q51700 (NIRS_PARDP)

Names and origin

Protein names

Recommended name:
    Nitrite reductase
    EC=1.7.2.1
Alternative name(s):
    Cytochrome cd1
    Cytochrome oxidase
    Hydroxylamine reductase
    EC=1.7.99.1

Gene names

Name:	nirS
Ordered Locus Names:	Pden_2487

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Inactivation of this cytochrome oxidase results in the loss of nitrite and nitric oxide reductase activities, but not of nitrous oxide reductase activity.
Catalytic activity	Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+. NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.
Cofactor	Binds 2 heme groups per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Contains 1 cytochrome c domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro hydroxylamine reductase activity Inferred from electronic annotation. Source: EC nitrite reductase (NO-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	By similarity
	Chain	30 – 596	567	Nitrite reductase		PRO_0000006574
Regions
	Domain	77 – 162	86	Cytochrome c
	Region	30 – 76	47	N-terminal tail
	Region	163 – 596	434	D1-heme domain
Sites
	Metal binding	46	1	Iron (heme axial ligand) By similarity
	Metal binding	98	1	Iron (heme axial ligand) By similarity
	Metal binding	229	1	Iron (heme D1 proximal ligand) By similarity
	Binding site	94	1	Heme (covalent) By similarity
	Binding site	97	1	Heme (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51700-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 90C981B688A74BD3	FASTA	596	65,440
	10 20 30 40 50 60 MRQRTPFARP GLLASAALAL VLGPLAVAAQ EQAAPPKDPA AALEDHKTKT DNRYEPSLDN 70 80 90 100 110 120 LAQQDVAALG APEGIPALSD AQYNEANKIY FERCAGCHGV LRKGATGKAL TPDLTRDLGF 130 140 150 160 170 180 DYLQSFITYG SPAGMPNWGT SGELTAEQVD LMANYLLLDP AAPPEFGMKE MRESWQVHVA 190 200 210 220 230 240 PEDRPTQQEN DWDLENLFSV TLRDAGQIAL IDGTTYEIKS VLDTGYAVHI SRMSASGRYL 250 260 270 280 290 300 FVIGRDGKVN MIDLWMKEPA TVAEIKIGSE ARSIETSKME GWEDKYAIAG AYWPPQYVIM 310 320 330 340 350 360 DGETLEPMKI QSTRGMIYDE QEYHPEPRVA AILASHYRPE FIVNVKETGK ILLVDYTDLK 370 380 390 400 410 420 NLKTTEIEAE RFLHDGGLDG SHRYFITAAN ARNKLVVIDT KEGKLVAIED TGGQTPHPGR 430 440 450 460 470 480 GANFVHPTFG PVWATSHMGD DSVALIGTDP EGHPDNAWKI LDSFPALGGG SLFIKTHPNS 490 500 510 520 530 540 QYLYVDATLN PEAEISGSVA VFDTKAMTGD GSDPEFKTLP IAEWAGIAEG QPRVVQGEFN 550 560 570 580 590 KDGTEVWFSV WNGKDQESAL VVVDDKTLEL KHVIKDERLV TPTGKFNVYN TMTDTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, sequencing and mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitrificans." de Boer A.P.N., Reijnders W.N.M., Kuenen J.G., Stouthamer A.H., van Spanning R.J.M. Antonie Van Leeuwenhoek 66:111-127(1994) [PubMed: 7747927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05002 Genomic DNA. Translation: AAA93118.1. CP000489 Genomic DNA. Translation: ABL70574.1.
RefSeq	YP_916270.1.
3D structure databases
HSSP	HSSP built from PDB template 1H9X based on UniProtKB P72181.
SMR	Q51700. Positions 38-596.
ModBase	Search...
Genome annotation databases
GeneID	4580125.
GenomeReviews	Gene locus Pden_2487 in contig CP000489_GR.
KEGG	pde:Pden_2487.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q51700. MSASGRY.
Family and domain databases
InterPro	IPR003088. Cyt_c_I. IPR009056. Cyt_c_monohaem. IPR003143. Cyt_d1_haem. [Graphical view]
Gene3D	G3DSA:2.140.10.20. Cyt_d1_haem. 1 hit. G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
Pfam	PF00034. Cytochrom_C. 1 hit. PF02239. Cytochrom_D1. 1 hit. [Graphical view]
PROSITE	PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIRS_PARDP

Accession

Primary (citable) accession number: Q51700
Secondary accession number(s): A1B4Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)