Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q51700

- NIRS_PARDP

UniProt

Q51700 - NIRS_PARDP

Protein

Nitrite reductase

Gene

nirS

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Inactivation of this cytochrome oxidase results in the loss of nitrite and nitric oxide reductase activities, but not of nitrous oxide reductase activity.

    Catalytic activityi

    Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.
    NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

    Cofactori

    Binds 2 heme groups per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi46 – 461Iron (heme axial ligand)PROSITE-ProRule annotation
    Binding sitei94 – 941Heme (covalent)PROSITE-ProRule annotation
    Binding sitei97 – 971Heme (covalent)PROSITE-ProRule annotation
    Metal bindingi98 – 981Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi229 – 2291Iron (heme D1 proximal ligand)By similarity

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. hydroxylamine reductase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW
    5. nitrite reductase (NO-forming) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciPDEN318586:GCVQ-2528-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrite reductase (EC:1.7.2.1)
    Alternative name(s):
    Cytochrome cd1
    Cytochrome oxidase
    Hydroxylamine reductase (EC:1.7.99.1)
    Gene namesi
    Name:nirS
    Ordered Locus Names:Pden_2487
    OrganismiParacoccus denitrificans (strain Pd 1222)
    Taxonomic identifieri318586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
    ProteomesiUP000000361: Chromosome 1

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929By similarityAdd
    BLAST
    Chaini30 – 596567Nitrite reductasePRO_0000006574Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi318586.Pden_2487.

    Structurei

    3D structure databases

    ProteinModelPortaliQ51700.
    SMRiQ51700. Positions 38-596.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 16286Cytochrome cPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni30 – 7647N-terminal tailAdd
    BLAST
    Regioni163 – 596434D1-heme domainAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cytochrome c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2010.
    HOGENOMiHOG000225986.
    KOiK15864.
    OMAiWPPQFVI.
    OrthoDBiEOG6RC3M0.

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    2.130.10.10. 1 hit.
    2.140.10.20. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR003143. Cyt_cd1_C.
    IPR011048. Haem_d1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PfamiPF13442. Cytochrome_CBB3. 1 hit.
    [Graphical view]
    SUPFAMiSSF46626. SSF46626. 2 hits.
    SSF51004. SSF51004. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q51700-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQRTPFARP GLLASAALAL VLGPLAVAAQ EQAAPPKDPA AALEDHKTKT    50
    DNRYEPSLDN LAQQDVAALG APEGIPALSD AQYNEANKIY FERCAGCHGV 100
    LRKGATGKAL TPDLTRDLGF DYLQSFITYG SPAGMPNWGT SGELTAEQVD 150
    LMANYLLLDP AAPPEFGMKE MRESWQVHVA PEDRPTQQEN DWDLENLFSV 200
    TLRDAGQIAL IDGTTYEIKS VLDTGYAVHI SRMSASGRYL FVIGRDGKVN 250
    MIDLWMKEPA TVAEIKIGSE ARSIETSKME GWEDKYAIAG AYWPPQYVIM 300
    DGETLEPMKI QSTRGMIYDE QEYHPEPRVA AILASHYRPE FIVNVKETGK 350
    ILLVDYTDLK NLKTTEIEAE RFLHDGGLDG SHRYFITAAN ARNKLVVIDT 400
    KEGKLVAIED TGGQTPHPGR GANFVHPTFG PVWATSHMGD DSVALIGTDP 450
    EGHPDNAWKI LDSFPALGGG SLFIKTHPNS QYLYVDATLN PEAEISGSVA 500
    VFDTKAMTGD GSDPEFKTLP IAEWAGIAEG QPRVVQGEFN KDGTEVWFSV 550
    WNGKDQESAL VVVDDKTLEL KHVIKDERLV TPTGKFNVYN TMTDTY 596
    Length:596
    Mass (Da):65,440
    Last modified:November 1, 1996 - v1
    Checksum:i90C981B688A74BD3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05002 Genomic DNA. Translation: AAA93118.1.
    CP000489 Genomic DNA. Translation: ABL70574.1.
    RefSeqiWP_011748767.1. NC_008686.1.
    YP_916270.1. NC_008686.1.

    Genome annotation databases

    EnsemblBacteriaiABL70574; ABL70574; Pden_2487.
    GeneIDi4580125.
    KEGGipde:Pden_2487.
    PATRICi22856095. VBIParDen97112_2412.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05002 Genomic DNA. Translation: AAA93118.1 .
    CP000489 Genomic DNA. Translation: ABL70574.1 .
    RefSeqi WP_011748767.1. NC_008686.1.
    YP_916270.1. NC_008686.1.

    3D structure databases

    ProteinModelPortali Q51700.
    SMRi Q51700. Positions 38-596.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 318586.Pden_2487.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL70574 ; ABL70574 ; Pden_2487 .
    GeneIDi 4580125.
    KEGGi pde:Pden_2487.
    PATRICi 22856095. VBIParDen97112_2412.

    Phylogenomic databases

    eggNOGi COG2010.
    HOGENOMi HOG000225986.
    KOi K15864.
    OMAi WPPQFVI.
    OrthoDBi EOG6RC3M0.

    Enzyme and pathway databases

    BioCyci PDEN318586:GCVQ-2528-MONOMER.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    2.130.10.10. 1 hit.
    2.140.10.20. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR003143. Cyt_cd1_C.
    IPR011048. Haem_d1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    Pfami PF13442. Cytochrome_CBB3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46626. SSF46626. 2 hits.
    SSF51004. SSF51004. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequencing and mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitrificans."
      de Boer A.P.N., Reijnders W.N.M., Kuenen J.G., Stouthamer A.H., van Spanning R.J.M.
      Antonie Van Leeuwenhoek 66:111-127(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pd 1222.

    Entry informationi

    Entry nameiNIRS_PARDP
    AccessioniPrimary (citable) accession number: Q51700
    Secondary accession number(s): A1B4Y0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3