Q51698 (IORB_BREDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoquinoline 1-oxidoreductase subunit beta
EC=1.3.99.16

Gene names

Name:

iorB

Organism

Brevundimonas diminuta (Pseudomonas diminuta)

Taxonomic identifier

293 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Brevundimonas

Protein attributes

Sequence length	781 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specific towards N-containing N-heterocyclic substrates, including isoquinoline, isoquinolin-5-ol, phthalazine and quinazoline.
Catalytic activity	Isoquinoline + acceptor + H2O = isoquinolin-1(2H)-one + reduced acceptor.
Subunit structure	Heterodimer of an alpha chain and a beta chain.

Ontologies

Keywords
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	isoquinoline 1-oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 781	781	Isoquinoline 1-oxidoreductase subunit beta		PRO_0000084216

Sequences

	Sequence		Length	Mass (Da)
	Q51698 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 45F9B3AEC7AA4F57	FASTA	781	84,483
	10 20 30 40 50 60 MKTVLPSVPE TVRLSRRGFL VQAGTITCSV AFGSVPAAAG DTAESTPSIA AVSPNVWVRV 70 80 90 100 110 120 HADGIVDIVC PAVELGQGAH TALPRFVAEE LDADWDRVRV QQAGASDKVY GNPLAWGTQF 130 140 150 160 170 180 TAASRTTVGY FDVLRVAGAQ ARFVLVQTAA RRWSVPADQL ETQKGVVLHR RSRRSATYGE 190 200 210 220 230 240 LVASVQVPES FPHFFARNEA TQPADDYFGA APPSVVAQAA GPASGAIALK HRSTYRLIGK 250 260 270 280 290 300 DAPRKDIPPK VNGQACYGMD VQVPGMLYAM VETGPVAGMA PERVDDGAAR QVPGIHHVLS 310 320 330 340 350 360 LPHGVAVVGR DIFAVRAARA RLLVNWKANP DKQSYDSGQV LDEFSDLCRN GIERNAVQAW 370 380 390 400 410 420 KQGELSSIDA VFARPDVRIE SFEMQSDLVY QAPMEPQSAV IQPHADGSAE AWVGTQWPTV 430 440 450 460 470 480 EQGFAAGILG IAPDKLTMHL PLVGGGFGRR LEPGALVDAA HIVRAIGKTV KVIWSREDDL 490 500 510 520 530 540 KRNPFRQALA CRVEAAVLEK DQRILALRHT VAADSWLARL FPQYFNAYQQ TDPGNWIGGM 550 560 570 580 590 600 VAYDVPLQRI DALTPRRSVD VCYMRGIGVA QVKFAQESLV DQIARRLNAD PVDFRLAHLN 610 620 630 640 650 660 TSPRGAAVVR TVAEMSDWKR RSADAGGGMA LGLAYTPYSN AHVALVSEVH FNRSENTLSV 670 680 690 700 710 720 SRVWCAVDVG MVAQPDIVKA QMEGGIIQGL SVALMERVQV AKGVLQHSNF HDYPMLRMSQ 730 740 750 760 770 780 VPQIHVRLVE TDQAMAGVAE LGLLQIGPAI NNAFARITGQ HLRSLPMRPA LAQMKRSGPT A

References

[1]	"Molecular cloning of the isoquinoline 1-oxidoreductase genes from Pseudomonas diminuta 7, structural analysis of iorA and iorB, and sequence comparisons with other molybdenum-containing hydroxylases." Lehmann M., Tshisuaka B., Fetzner S., Lingens F.Y. J. Biol. Chem. 270:14420-14429(1995) [PubMed: 7782304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 7.
[2]	"Purification and characterization of isoquinoline 1-oxidoreductase from Pseudomonas diminuta 7, a novel molybdenum-containing hydroxylase." Lehmann M., Tshisuaka B., Fetzner S., Roger P., Lingens F.Y. J. Biol. Chem. 269:11254-11260(1994) [PubMed: 8157655] [Abstract] Cited for: CHARACTERIZATION. Strain: 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48918 Genomic DNA. Translation: CAA88754.1.
PIR	B56939.
3D structure databases
ProteinModelPortal	Q51698.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	1.3.99.16. 982.
Family and domain databases
InterPro	IPR000674. Ald_Oxase/Xan_DH_a/b. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR012368. OxRdtase_Mopterin-bd_su_IorB. IPR006311. TAT_signal. [Graphical view]
Gene3D	G3DSA:3.30.365.10. Ald_xan_DH_mo_bd. 4 hits. G3DSA:3.90.1170.50. Aldxan_DH_hamm. 1 hit.
Pfam	PF02738. Ald_Xan_dh_C2. 3 hits. [Graphical view]
PIRSF	PIRSF036389. IOR_B. 1 hit.
SMART	SM01008. Ald_Xan_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF56003. Ald_xan_DH_mo_bd. 2 hits. SSF54665. Aldxan_dh_hamm. 1 hit.
PROSITE	PS51318. TAT. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IORB_BREDI

Accession

Primary (citable) accession number: Q51698

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program