Reviewed, UniProtKB/Swiss-Prot Q51676 (HEMN_PARDP)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen-III oxidase
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemN
Ordered Locus Names:	Pden_1851

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX By similarity.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (AdoMet route): step 1/1.
Subunit structure	Monomer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Oxygen-independent coproporphyrinogen-III oxidase		PRO_0000109945
Regions
	Region	112 – 113	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	60	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	64	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	67	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	54	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	66	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	111	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	144	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	171	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	183	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	208	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51676-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D424AA04E4B4519A	FASTA	451	49,848
	10 20 30 40 50 60 MEQQSQLERL GLFDARVPRY TSYPTAPHFT PAVGEPVFRD WIAAIPAGAA ISLYMHVPFC 70 80 90 100 110 120 RRLCWFCACR TQGTQSDEPV RAYAKALLAE LDMLKSALAP GVRLSRLHWG GGTPTLMPAE 130 140 150 160 170 180 MMRLVAGAVL DAFPLAEGAE FSVEIDPNEI DEARMDALAE AGLNRASIGV QDFDPEIQKI 190 200 210 220 230 240 IGREQSFEVT KRAVDMIRDR GIASLNADIL YGLPHQDPHR IAESVQKLLA LSPDRVALYG 250 260 270 280 290 300 YAHVPWMAKR QVMIPSEALP DPHGRLRLFE TARELFLADG YDEIGIDHFA RPGDGLARAQ 310 320 330 340 350 360 KAGLLRRNFQ GYTDDRAEVL VGLGASSISR FPQGYAQNAP ATGAHLARIR DGRFSTTRGH 370 380 390 400 410 420 AFSAEDRWRS RMIEALMCDF EIRAEEFIRD HGFDAESLSR ILTPVAAHFG DMVDADASGL 430 440 450 RITPRGRPLT RMIARMFDGY DMAASGHSAA I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and functional analysis of aa3-type and cbb3-type cytochrome c oxidases of Paracoccus denitrificans reveals significant differences in proton-pump design." de Gier J.W., Schepper M., Reijnders W.N.M., van Dyck S.J., Slotboom D.J., Warne A., Saraste M., Krab K., Finel M., Stouthamer A.H., van Spanning R.J.M., der Oost J. Mol. Microbiol. 20:1247-1260(1996) [PubMed: 8809776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U34353 Genomic DNA. Translation: AAC44513.1. CP000489 Genomic DNA. Translation: ABL69948.1.
PIR	S77599.
RefSeq	YP_915644.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4581035.
GenomeReviews	Gene locus Pden_1851 in contig CP000489_GR.
KEGG	pde:Pden_1851.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q51676. ISRFPQG.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR004558. HemN. IPR010723. HemN_C. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF06969. HemN_C. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00538. hemN. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEMN_PARDP

Accession

Primary (citable) accession number: Q51676
Secondary accession number(s): A1B354

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)