Q51602 (CBDB_BURCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-halobenzoate 1,2-dioxygenase small subunit
EC=1.14.12.13

Alternative name(s):
2-chlorobenzoate 1,2-dioxygenase

Gene names

Name:

cbdB

Encoded on

Plasmid pBAH1

Organism

Burkholderia cepacia (Pseudomonas cepacia)

Taxonomic identifier

292 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol.
Catalytic activity	2-chlorobenzoate + NADH + O2 = catechol + chloride + NAD+ + CO2.
Pathway	Xenobiotic degradation; benzoate degradation via CoA ligation.
Subunit structure	Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB) subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two component enzyme system. The other component is a NADH:acceptor reductase (CdbC).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-chlorobenzoate 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 165	164	2-halobenzoate 1,2-dioxygenase small subunit		PRO_0000085071
Experimental info
	Sequence conflict	12	1	V → C in CAA55682. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q51602 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4886AF20AE127AA8	FASTA	165	19,632
	10 20 30 40 50 60 MTSLESSYLD VVAFIFREAR LLDDRSWDEW LECYDPEAVF WMPCWDDADT LVDDPRKHVS 70 80 90 100 110 120 LIYYSDRMGL EDRVFRLRSE RSGASTPEPR TTHNIANVEI LERTERQIEA RFNWHTMNYR 130 140 150 160 YKLLDHYFGT SFYTLKVSSS GLSILNKKVV LKNDLIHQVI DVYHV

References

[1]	"Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS." Haak B., Fetzner S., Lingens F. J. Bacteriol. 177:667-675(1995) [PubMed: 7530709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2CBS.
[2]	"Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS." Fetzner S., Mueller R., Lingens F. J. Bacteriol. 174:279-290(1992) [PubMed: 1370284] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-15, CHARACTERIZATION. Strain: 2CBS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79076 Genomic DNA. Translation: CAA55682.1.
3D structure databases
ProteinModelPortal	Q51602.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14764.
Family and domain databases
InterPro	IPR017641. Benzo_1-2-diOase_ssu. IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03232. Benzo_1_2_benB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CBDB_BURCE

Accession

Primary (citable) accession number: Q51602
Secondary accession number(s): O08067

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	January 23, 2007
Last modified:	June 28, 2011
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program