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Q51601 (CBDA_BURCE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-halobenzoate 1,2-dioxygenase large subunit

EC=1.14.12.13
Alternative name(s):
2-chlorobenzoate 1,2-dioxygenase
Gene names
Name:cbdA
Encoded onPlasmid pBAH1
OrganismBurkholderia cepacia (Pseudomonas cepacia)
Taxonomic identifier292 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol.

Catalytic activity

A 2-halobenzoate + NADH + O2 = catechol + a halide anion + NAD+ + CO2.

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Binds 1 Fe2+ ion per subunit By similarity.

Pathway

Xenobiotic degradation; benzoate degradation via CoA ligation.

Subunit structure

Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB) subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two component enzyme system. The other component is a NADH:acceptor reductase (CdbC).

Sequence similarities

Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.

Contains 1 Rieske domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 4654642-halobenzoate 1,2-dioxygenase large subunit
PRO_0000085051

Regions

Domain56 – 15499Rieske

Sites

Metal binding981Iron-sulfur (2Fe-2S) By similarity
Metal binding1001Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal binding1181Iron-sulfur (2Fe-2S) By similarity
Metal binding1211Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal binding2271Iron By similarity
Metal binding2321Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q51601 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 08747DF6FB8D30DC

FASTA46552,478
        10         20         30         40         50         60 
MSTPLIAGTG PSAVRQLISN AVQNDPVSGN FRCRRDIFTD AALFDYEMKY IFEQNWVFLA 

        70         80         90        100        110        120 
HESQVANPDD YLVSNIGRQP VIITRNKAGD VSAVINACSH RGAELCRRKQ GNRSTFTCQF 

       130        140        150        160        170        180 
HGWTFSNTGK LLKVKDGQDD NYPEGFNVDG SHDLTRIPSF ANYRGFLFGS MNPDACPIEE 

       190        200        210        220        230        240 
HLGGSKAILD QVIDQTPGEL EVLRGSSSYI YDGNWKLQIE NGADGYHVGS VHWNYVATIG 

       250        260        270        280        290        300 
RRDRTSDTIR TVDVTTWSKK NIGGTYTFEH GHMLLWTRLP NPEVRPVFAR REELKARVGE 

       310        320        330        340        350        360 
EVADAIVNQT RNLCIYPNLY VMDQISTQIR VVRPISVDKT EVTIYCFAPR DESEEVRNAR 

       370        380        390        400        410        420 
IRQYEDFFNV SGMGTPDDLE EFRACQSGYR GSAREWNDLS RGAPHWISGP DDNARRLGLA 

       430        440        450        460 
PLMSGARMED EGLFVQQHTY WAETMLRGIE AEPKVFNVQP VEVAQ 

« Hide

References

[1]"Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS."
Haak B., Fetzner S., Lingens F.
J. Bacteriol. 177:667-675(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 2CBS.
[2]"Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS."
Fetzner S., Mueller R., Lingens F.
J. Bacteriol. 174:279-290(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-21, CHARACTERIZATION.
Strain: 2CBS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79076 Genomic DNA. Translation: CAA55681.1.

3D structure databases

ProteinModelPortalQ51601.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-14763.
UniPathwayUPA00233.

Family and domain databases

Gene3D2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSPR00090. RNGDIOXGNASE.
SUPFAMSSF50022. SSF50022. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBDA_BURCE
AccessionPrimary (citable) accession number: Q51601
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways