Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q51601

- CBDA_BURCE

UniProt

Q51601 - CBDA_BURCE

Protein

2-halobenzoate 1,2-dioxygenase large subunit

Gene

cbdA

Organism
Burkholderia cepacia (Pseudomonas cepacia)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol.

    Catalytic activityi

    A 2-halobenzoate + NADH + O2 = catechol + a halide anion + NAD+ + CO2.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.PROSITE-ProRule annotation
    Binds 1 Fe2+ ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi98 – 981Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi100 – 1001Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
    Metal bindingi118 – 1181Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
    Metal bindingi121 – 1211Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
    Metal bindingi227 – 2271IronBy similarity
    Metal bindingi232 – 2321IronBy similarity

    GO - Molecular functioni

    1. 2-chlorobenzoate 1,2-dioxygenase activity Source: UniProtKB-EC
    2. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. benzoate catabolic process via CoA ligation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14763.
    UniPathwayiUPA00233.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-halobenzoate 1,2-dioxygenase large subunit (EC:1.14.12.13)
    Alternative name(s):
    2-chlorobenzoate 1,2-dioxygenase
    Gene namesi
    Name:cbdA
    Encoded oniPlasmid pBAH10 Publication
    OrganismiBurkholderia cepacia (Pseudomonas cepacia)
    Taxonomic identifieri292 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 4654642-halobenzoate 1,2-dioxygenase large subunitPRO_0000085051Add
    BLAST

    Interactioni

    Subunit structurei

    Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB) subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two component enzyme system. The other component is a NADH:acceptor reductase (CdbC).

    Structurei

    3D structure databases

    ProteinModelPortaliQ51601.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 15499RieskePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rieske domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.102.10.10. 1 hit.
    3.90.380.10. 1 hit.
    InterProiIPR017941. Rieske_2Fe-2S.
    IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
    IPR015879. Ring_hydroxy_dOase_asu_C_dom.
    IPR001663. Rng_hydr_dOase-A.
    [Graphical view]
    PfamiPF00355. Rieske. 1 hit.
    PF00848. Ring_hydroxyl_A. 1 hit.
    [Graphical view]
    PRINTSiPR00090. RNGDIOXGNASE.
    SUPFAMiSSF50022. SSF50022. 1 hit.
    PROSITEiPS51296. RIESKE. 1 hit.
    PS00570. RING_HYDROXYL_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q51601-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPLIAGTG PSAVRQLISN AVQNDPVSGN FRCRRDIFTD AALFDYEMKY    50
    IFEQNWVFLA HESQVANPDD YLVSNIGRQP VIITRNKAGD VSAVINACSH 100
    RGAELCRRKQ GNRSTFTCQF HGWTFSNTGK LLKVKDGQDD NYPEGFNVDG 150
    SHDLTRIPSF ANYRGFLFGS MNPDACPIEE HLGGSKAILD QVIDQTPGEL 200
    EVLRGSSSYI YDGNWKLQIE NGADGYHVGS VHWNYVATIG RRDRTSDTIR 250
    TVDVTTWSKK NIGGTYTFEH GHMLLWTRLP NPEVRPVFAR REELKARVGE 300
    EVADAIVNQT RNLCIYPNLY VMDQISTQIR VVRPISVDKT EVTIYCFAPR 350
    DESEEVRNAR IRQYEDFFNV SGMGTPDDLE EFRACQSGYR GSAREWNDLS 400
    RGAPHWISGP DDNARRLGLA PLMSGARMED EGLFVQQHTY WAETMLRGIE 450
    AEPKVFNVQP VEVAQ 465
    Length:465
    Mass (Da):52,478
    Last modified:January 23, 2007 - v3
    Checksum:i08747DF6FB8D30DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79076 Genomic DNA. Translation: CAA55681.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79076 Genomic DNA. Translation: CAA55681.1 .

    3D structure databases

    ProteinModelPortali Q51601.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00233 .
    BioCyci MetaCyc:MONOMER-14763.

    Family and domain databases

    Gene3Di 2.102.10.10. 1 hit.
    3.90.380.10. 1 hit.
    InterProi IPR017941. Rieske_2Fe-2S.
    IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
    IPR015879. Ring_hydroxy_dOase_asu_C_dom.
    IPR001663. Rng_hydr_dOase-A.
    [Graphical view ]
    Pfami PF00355. Rieske. 1 hit.
    PF00848. Ring_hydroxyl_A. 1 hit.
    [Graphical view ]
    PRINTSi PR00090. RNGDIOXGNASE.
    SUPFAMi SSF50022. SSF50022. 1 hit.
    PROSITEi PS51296. RIESKE. 1 hit.
    PS00570. RING_HYDROXYL_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS."
      Haak B., Fetzner S., Lingens F.
      J. Bacteriol. 177:667-675(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 2CBS.
    2. "Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS."
      Fetzner S., Mueller R., Lingens F.
      J. Bacteriol. 174:279-290(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-21, CHARACTERIZATION.
      Strain: 2CBS.

    Entry informationi

    Entry nameiCBDA_BURCE
    AccessioniPrimary (citable) accession number: Q51601
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3