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Protein

2-halobenzoate 1,2-dioxygenase large subunit

Gene

cbdA

Organism
Burkholderia cepacia (Pseudomonas cepacia)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol.

Catalytic activityi

A 2-halobenzoate + NADH + O2 = catechol + a halide anion + NAD+ + CO2.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation
  • Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Pathway: benzoate degradation via CoA ligation

This protein is involved in the pathway benzoate degradation via CoA ligation, which is part of Xenobiotic degradation.
View all proteins of this organism that are known to be involved in the pathway benzoate degradation via CoA ligation and in Xenobiotic degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi98 – 981Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi100 – 1001Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi118 – 1181Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi121 – 1211Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi227 – 2271IronBy similarity
Metal bindingi232 – 2321IronBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14763.
UniPathwayiUPA00233.

Names & Taxonomyi

Protein namesi
Recommended name:
2-halobenzoate 1,2-dioxygenase large subunit (EC:1.14.12.13)
Alternative name(s):
2-chlorobenzoate 1,2-dioxygenase
Gene namesi
Name:cbdA
Encoded oniPlasmid pBAH10 Publication
OrganismiBurkholderia cepacia (Pseudomonas cepacia)
Taxonomic identifieri292 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 4654642-halobenzoate 1,2-dioxygenase large subunitPRO_0000085051Add
BLAST

Interactioni

Subunit structurei

Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB) subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two component enzyme system. The other component is a NADH:acceptor reductase (CdbC).

Structurei

3D structure databases

ProteinModelPortaliQ51601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 15499RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q51601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPLIAGTG PSAVRQLISN AVQNDPVSGN FRCRRDIFTD AALFDYEMKY
60 70 80 90 100
IFEQNWVFLA HESQVANPDD YLVSNIGRQP VIITRNKAGD VSAVINACSH
110 120 130 140 150
RGAELCRRKQ GNRSTFTCQF HGWTFSNTGK LLKVKDGQDD NYPEGFNVDG
160 170 180 190 200
SHDLTRIPSF ANYRGFLFGS MNPDACPIEE HLGGSKAILD QVIDQTPGEL
210 220 230 240 250
EVLRGSSSYI YDGNWKLQIE NGADGYHVGS VHWNYVATIG RRDRTSDTIR
260 270 280 290 300
TVDVTTWSKK NIGGTYTFEH GHMLLWTRLP NPEVRPVFAR REELKARVGE
310 320 330 340 350
EVADAIVNQT RNLCIYPNLY VMDQISTQIR VVRPISVDKT EVTIYCFAPR
360 370 380 390 400
DESEEVRNAR IRQYEDFFNV SGMGTPDDLE EFRACQSGYR GSAREWNDLS
410 420 430 440 450
RGAPHWISGP DDNARRLGLA PLMSGARMED EGLFVQQHTY WAETMLRGIE
460
AEPKVFNVQP VEVAQ
Length:465
Mass (Da):52,478
Last modified:January 23, 2007 - v3
Checksum:i08747DF6FB8D30DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79076 Genomic DNA. Translation: CAA55681.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79076 Genomic DNA. Translation: CAA55681.1.

3D structure databases

ProteinModelPortaliQ51601.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00233.
BioCyciMetaCyc:MONOMER-14763.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 1 hit.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015881. Ring-hydroxy_dOase_2Fe2S_BS.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
PS00570. RING_HYDROXYL_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS."
    Haak B., Fetzner S., Lingens F.
    J. Bacteriol. 177:667-675(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 2CBS.
  2. "Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS."
    Fetzner S., Mueller R., Lingens F.
    J. Bacteriol. 174:279-290(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21, CHARACTERIZATION.
    Strain: 2CBS.

Entry informationi

Entry nameiCBDA_BURCE
AccessioniPrimary (citable) accession number: Q51601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.