Q51574 (BLO15_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase OXA-15
EC=3.5.2.6

Gene names

Name:	bla
Synonyms:	oxa15

Encoded on

Plasmid pMLH54

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes oxacillin, first-generation cephalosporins and ceftazidime. Does not hydrolyze cefotaxime or carbapenems.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-D beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro peptidoglycan-based cell wall biogenesis Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC penicillin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Chain	22 – 275	254	Beta-lactamase OXA-15		PRO_0000017032
Regions
	Region	210 – 212	3	Substrate binding By similarity
Sites
	Active site	72	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	75	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51574 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D3678DFE678C38B5	FASTA	275	31,628
	10 20 30 40 50 60 MAIRIFAILF SIFSLATFAH AQEGTLERSD WRKFFSEFQA KGTIVVADER QADRAMLVFD 70 80 90 100 110 120 PVRSKKRYSP ASTFKIPHTL FALDAGAVRD EFQIFRWDGV NRGFAGHNQD QDLRSAMRNS 130 140 150 160 170 180 TVWVYELFAK EIGDDKARRY LKKIDYGNAG PSTSNGDYWI EGSLAISAQE QIAFLRKLYR 190 200 210 220 230 240 NELPFRVEHQ RLVKDLMIVE AGRNWILRAK TGWEGRMGWW VGWVEWPTGS VFFALNIDTP 250 260 270 NRMDDLFKRE AIVRAILRSI EALPPNPAVN SDAAR

References

[1]

"OXA-15, an extended-spectrum variant of OXA-2 beta-lactamase, isolated from a Pseudomonas aeruginosa strain." Danel F., Hall L.M.C., Gur D., Livermore D.M. Antimicrob. Agents Chemother. 41:785-790(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: AH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63835 Genomic DNA. Translation: AAB05874.1.
3D structure databases
ProteinModelPortal	Q51574.
SMR	Q51574. Positions 25-265.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR002137. Beta-lactam_class-D_AS. IPR001460. PCN-bd_Tpept. [Graphical view]
Pfam	PF00905. Transpeptidase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00337. BETA_LACTAMASE_D. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLO15_PSEAI

Accession

Primary (citable) accession number: Q51574

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program