Q51519 (PHZB_PSECL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase, phenazine specific
EC=4.1.3.27

Including the following 1 domains:

1. Glutamine amidotransferase

Gene names

Name:

phzB

Organism

Pseudomonas chlororaphis (Pseudomonas aureofaciens)

Taxonomic identifier

333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Involved in the biosynthesis of the antibiotic, phenazine, a nitrogen-containing heterocyclic molecule having important roles in virulence, competition and biological control.
Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Antibiotic biosynthesis; phenazine biosynthesis.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis Virulence
Domain	Glutamine amidotransferase
Molecular function	Lyase Transferase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Anthranilate synthase, phenazine specific		PRO_0000056907
Regions
	Domain	437 – 628	192	Glutamine amidotransferase type-1
	Region	1 – 434	434	Anthranilate synthase component I
Sites
	Active site	517	1	For GATase activity By similarity
	Active site	602	1	For GATase activity By similarity
	Active site	604	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51519 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 23ECC15C1455D2C7	FASTA	637	69,878
	10 20 30 40 50 60 MSQTAAHLME RILQPAPEPF ALLYRPESSG PGLLDVLIGE MSEPQVLADI DLPATSIGAP 70 80 90 100 110 120 RLDVLALIPY RQIAERGFEA VDDESPLLAM NITEQQSISI ERLLGMLPNV PIQLNSERFD 130 140 150 160 170 180 LSDASYAEIV SQVIANEIGS GEGANFVIKR TFLAEISEYG PASALSFFRH LLEREKGAYW 190 200 210 220 230 240 TFIIHTGSRT FVGASPERHI SIKDGLSVMN PISGTYRYPP AGPNLSEVMD FLADRKEADE 250 260 270 280 290 300 LYMVVDEELK MMARICEDGG HVLGPYLKEM AHLAHTEYFI EGKTHRDVRE ILRETLFAPT 310 320 330 340 350 360 VTGSPLESAC RVIQRYEPQG RAYYSGMAAL IGSDGKGGRS LDSAILIRTA DIDNSGEVRI 370 380 390 400 410 420 SVGSTIVRHS DPMTEAAESR AKATGLISAL KNQAPSRFGN HLQVRAALAS RNAYVSDFWL 430 440 450 460 470 480 MDSQQREQIQ ADFSGRQVLI VDAEDTFTSM IAKQLRALGL VVTVCSFSDE YSFEGYDLVI 490 500 510 520 530 540 MGPGPGNPSE VQQPKINHLH VAIRSLLSQQ RPFLAVCLSH QVLSLCLGLE LQRKAIPNQG 550 560 570 580 590 600 VQKQIDLFGN VERVGFYNTF AAQSSSDRLD IDGIGTVEIS RDSETGEVHA LRGPSFASMQ 610 620 630 FHAESLLTQE GPRIIADLLR HALIHTPVEN NASAAGR

References

[1]

"Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84." Pierson L.S. III, Gaffney T., Lam S., Gong F. FEMS Microbiol. Lett. 134:299-307(1995) [PubMed: 8586283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 30-84.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L48339 Genomic DNA. Translation: AAB00330.1.
3D structure databases
ProteinModelPortal	Q51519.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005801. ADC_synthase. IPR015890. Chorismate-bd_C. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.60.120.10. TRPE_1_chor_bd. 1 hit.
Pfam	PF00425. Chorismate_bind. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHZB_PSECL

Accession

Primary (citable) accession number: Q51519

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program