Reviewed, UniProtKB/Swiss-Prot Q51494 (NDOB_PSEAE)

Names and origin

Protein names

Recommended name:
    Naphthalene 1,2-dioxygenase subunit alpha
    EC=1.14.12.12
Alternative name(s):
    Naphthalene 1,2-dioxygenase ISP alpha

Gene names

Name:	ndoB
Synonyms:	pahA3

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol.
Catalytic activity	Naphthalene + NADH + O2 = (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+.
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Aromatic compound metabolism; naphtalene degradation.
Subunit structure	Naphthalene dioxygenase (NDO) multicomponent enzyme system is composed of an electron transfer component and an iron sulfur protein (ISP). The electron transfer component is composed of ferredoxin reductase (ndoR) and ferredoxin (ndoA), and ISP is composed of a large alpha subunit (ndoB) and a small beta subunit (ndoC).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naphthalene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Naphthalene 1,2-dioxygenase subunit alpha		PRO_0000085052
Regions
	Domain	39 – 137	99	Rieske
Sites
	Metal binding	81	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	83	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	101	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	104	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	208	1	Iron By similarity
	Metal binding	213	1	Iron By similarity
	Metal binding	362	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51494-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 35A189136722A21C	FASTA	449	49,716
	10 20 30 40 50 60 MNYKNKNLVS ESGLTQKHLI HGDEELFQRE LETIFARNWL FLTHDSLIPS PGDYVTAKMG 70 80 90 100 110 120 VDEVIVSRQN DGSIRAFLNV CRHRGKTLVH AEAGNAKGFV CSYHGWGFGA NGELQSVPFE 130 140 150 160 170 180 KELYGEALDK KCMGLKEVAR VESFHGFIYG CFDEEAPSLK DYMGDAGWYL EPMFKHSGGL 190 200 210 220 230 240 ELIGPPGKVI IKANWKAPAE NFTGDAYHVG WTHASSLRSG QSVFSSLAGN AALPPEGAGL 250 260 270 280 290 300 QMTSKYGSGM GVLWDGYSGV HSADLVPELM AFGGAKQERL NKEIGEVRAR IYRSHLNCTV 310 320 330 340 350 360 FPNNSFLTCS GVFKVWHPID ANTTEVWTYA MVEKDMPEDL KRRLVDAVQR TFGPAGFWES 370 380 390 400 410 420 DDNDNMETVS QNAKKYQSRD GDLVSNLGFG GDVYGDEVYP GIVGKSAIGE TSYRGFYRAY 430 440 GAHISSSSWA EFEDVSKNWH TELAKTTDR

References

[1]

"The molecular analysis of an NAH7-type gene cluster, pah, located on the chromosome of Pseudomonas aeruginosa PaK1." Takizawa N., Iida T., Yamauchi K., Satoh S., Wang Y., Fukuda M., Kiyohara H. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PaK1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84146 Genomic DNA. Translation: BAA12240.1.
3D structure databases
HSSP	HSSP built from PDB template 1O7N based on UniProtKB P23094.
SMR	Q51494. Positions 1-447.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.12.12. 354.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. IPR015879. Ring_hydroxy_dOase_asu_C. IPR001663. Rng_hydr_dOase-A. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR21266:SF2. Rng_hydr_dOase-A. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF00848. Ring_hydroxyl_A. 1 hit. [Graphical view]
PRINTS	PR00090. RNGDIOXGNASE.
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDOB_PSEAE

Accession

Primary (citable) accession number: Q51494

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)