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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81MagnesiumBy similarity
Metal bindingi67 – 671MagnesiumBy similarity
Metal bindingi139 – 1391MagnesiumBy similarity
Metal bindingi142 – 1421MagnesiumBy similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:PA0965
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0965.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174Crossover junction endodeoxyribonuclease RuvCPRO_0000183121Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0965.

Structurei

3D structure databases

ProteinModelPortaliQ51424.
SMRiQ51424. Positions 3-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.Curated

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
InParanoidiQ51424.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.
PhylomeDBiQ51424.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q51424-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLILGIDPG SRITGFGVVR ETARGCEYVA SGCIRTGNGP LHERLHVVFR
60 70 80 90 100
SVREVIRTHG PTALSIEQVF MARNADSALK LGQARGAAIV AAMEEGLSVA
110 120 130 140 150
EYTASQVKQA VVGTGGADKQ QVQMMVMHLL KLTQKPQIDA SDALAIALCH
160 170
AHTQQSLVPH GLVGARRRGG RLRL
Length:174
Mass (Da):18,557
Last modified:November 1, 1997 - v1
Checksum:iE639B12CEF5FF517
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83138 Genomic DNA. Translation: BAA11817.1.
AE004091 Genomic DNA. Translation: AAG04354.1.
PIRiJC5478.
RefSeqiNP_249656.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04354; AAG04354; PA0965.
GeneIDi882131.
KEGGipae:PA0965.
PATRICi19836230. VBIPseAer58763_1003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83138 Genomic DNA. Translation: BAA11817.1.
AE004091 Genomic DNA. Translation: AAG04354.1.
PIRiJC5478.
RefSeqiNP_249656.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ51424.
SMRiQ51424. Positions 3-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04354; AAG04354; PA0965.
GeneIDi882131.
KEGGipae:PA0965.
PATRICi19836230. VBIPseAer58763_1003.

Organism-specific databases

PseudoCAPiPA0965.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
InParanoidiQ51424.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.
PhylomeDBiQ51424.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates."
    Hishida T., Iwasaki H., Ishioka K., Shinagawa H.
    Gene 182:63-70(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRUVC_PSEAE
AccessioniPrimary (citable) accession number: Q51424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.