Q51404 (FUMC1_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II 1
Short name=Fumarase C 1
EC=4.2.1.2

Gene names

Name:	fumC1
Ordered Locus Names:	PA4470

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
Sequence caution	The sequence AAB17389.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Fumarate hydratase class II 1 HAMAP MF_00743		PRO_0000161299
Regions
	Region	123 – 126	4	B site By similarity
	Region	133 – 135	3	Substrate binding By similarity
Sites
	Binding site	100	1	Substrate By similarity
Experimental info
	Sequence conflict	156	1	P → S in AAB17389. Ref.2
	Sequence conflict	222	1	G → A in AAB17389. Ref.2
	Sequence conflict	331	1	A → G in AAB17389. Ref.2
	Sequence conflict	340	1	Missing in AAB17389. Ref.2
	Sequence conflict	362	1	N → T in AAB17389. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q51404 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 48D87C8B1C76B708	FASTA	458	48,696
	10 20 30 40 50 60 MTDTRIERDS MGELAVPATA LYGAQTQRAV NNFPVSGQRM PQAFVRALLL AKAAAARANV 70 80 90 100 110 120 SLQQLDAPMG EAIADTCLQL LQEDFMQHFP VDVFQTGSGT SSNMNANEVV ATLASRRLGG 130 140 150 160 170 180 KVNPNDHVNC GQSSNDIIPS TIHISAALEI SERLLPALRH LEQTIQSKAG EVHAYVKTGR 190 200 210 220 230 240 THLMDAMPVR MSQVLGGWAQ QVRQAGVHIE SVLPALQQLA QGGTAVGTGI NAHPRFAERF 250 260 270 280 290 300 SQELNDLTGL AFRPGDDFFA LIGSQDTAVA ASGQLKTLAV TLMKLANDLR WMNSGPLAGL 310 320 330 340 350 360 GEIELEALQP GSSIMPGKVN PVIPEATAMV AAQVIGNDAA IAVAGQSGNF ELNVMLPLVA 370 380 390 400 410 420 DNLLHSIQLL ANVSRLLADK AIASFKVNQG KLSEALARNP ILVTALNPII GYQKAAEIAK 430 440 450 QAYREGRPII DVALENTDLD RARLEVLLDP EKLTAGGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hassett D.J., Klotz M.G., Howell M.L. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FRD1.
[2]	"The Pseudomonas aeruginosa fumC and sodA genes belong to an iron-responsive operon." Polack B., Dacheux D., Delic-Attree I., Toussaint B., Vignais P.M. Biochem. Biophys. Res. Commun. 226:555-560(1996) [PubMed: 8806672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CHA.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59458 Genomic DNA. Translation: AAB02830.1. U72494 Genomic DNA. Translation: AAB17389.1. Different initiation. AE004091 Genomic DNA. Translation: AAG07858.1.
PIR	F83088. JC4982.
RefSeq	NP_253160.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q51404.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881033.
GenomeReviews	Gene locus PA4470 in contig AE004091_GR.
KEGG	pae:PA4470.
NMPDR	fig|208964.1.peg.4470.
PATRIC	19843667. VBIPseAer58763_4680.
Organism-specific databases
PseudoCAP	PA4470.
Phylogenomic databases
HOGENOM	HBG284369.
OMA	QVIGHHT.
ProtClustDB	PRK00485.
Enzyme and pathway databases
BioCyc	PAER208964:PA4470-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC1_PSEAE

Accession

Primary (citable) accession number: Q51404
Secondary accession number(s): P72168

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program