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Protein

Fumarate hydratase class II 2

Gene

fumC2

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II 2 (fumC2), Fumarate hydratase class II 1 (fumC1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei181SubstrateUniRule annotation1
Active sitei182Proton donor/acceptorUniRule annotation1
Active sitei312UniRule annotation1
Binding sitei313SubstrateUniRule annotation1
Sitei325Important for catalytic activityUniRule annotation1

GO - Molecular functioni

  • fumarate hydratase activity Source: PseudoCAP

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class II 2UniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase C 2UniRule annotation
Alternative name(s):
Aerobic fumarase 2UniRule annotation
Iron-independent fumarase 2UniRule annotation
Gene namesi
Name:fumC2UniRule annotation
Ordered Locus Names:PA4470
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4470.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612991 – 458Fumarate hydratase class II 2Add BLAST458

Proteomic databases

PaxDbiQ51404.
PRIDEiQ51404.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA4470.

Structurei

3D structure databases

ProteinModelPortaliQ51404.
SMRiQ51404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 100Substrate bindingUniRule annotation3
Regioni123 – 126Substrate binding (B site)UniRule annotation4
Regioni133 – 135Substrate bindingUniRule annotation3
Regioni318 – 320Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiQ51404.
KOiK01679.
OMAiRIATIWN.
PhylomeDBiQ51404.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q51404-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTRIERDS MGELAVPATA LYGAQTQRAV NNFPVSGQRM PQAFVRALLL
60 70 80 90 100
AKAAAARANV SLQQLDAPMG EAIADTCLQL LQEDFMQHFP VDVFQTGSGT
110 120 130 140 150
SSNMNANEVV ATLASRRLGG KVNPNDHVNC GQSSNDIIPS TIHISAALEI
160 170 180 190 200
SERLLPALRH LEQTIQSKAG EVHAYVKTGR THLMDAMPVR MSQVLGGWAQ
210 220 230 240 250
QVRQAGVHIE SVLPALQQLA QGGTAVGTGI NAHPRFAERF SQELNDLTGL
260 270 280 290 300
AFRPGDDFFA LIGSQDTAVA ASGQLKTLAV TLMKLANDLR WMNSGPLAGL
310 320 330 340 350
GEIELEALQP GSSIMPGKVN PVIPEATAMV AAQVIGNDAA IAVAGQSGNF
360 370 380 390 400
ELNVMLPLVA DNLLHSIQLL ANVSRLLADK AIASFKVNQG KLSEALARNP
410 420 430 440 450
ILVTALNPII GYQKAAEIAK QAYREGRPII DVALENTDLD RARLEVLLDP

EKLTAGGL
Length:458
Mass (Da):48,696
Last modified:November 1, 1996 - v1
Checksum:i48D87C8B1C76B708
GO

Sequence cautioni

The sequence AAB17389 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156P → S in AAB17389 (PubMed:8806672).Curated1
Sequence conflicti222G → A in AAB17389 (PubMed:8806672).Curated1
Sequence conflicti331A → G in AAB17389 (PubMed:8806672).Curated1
Sequence conflicti340Missing in AAB17389 (PubMed:8806672).Curated1
Sequence conflicti362N → T in AAB17389 (PubMed:8806672).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59458 Genomic DNA. Translation: AAB02830.1.
U72494 Genomic DNA. Translation: AAB17389.1. Different initiation.
AE004091 Genomic DNA. Translation: AAG07858.1.
PIRiF83088.
JC4982.
RefSeqiNP_253160.1. NC_002516.2.
WP_003112741.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG07858; AAG07858; PA4470.
GeneIDi881033.
KEGGipae:PA4470.
PATRICifig|208964.12.peg.4680.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFUMC2_PSEAE
AccessioniPrimary (citable) accession number: Q51404
Secondary accession number(s): P72168
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 5, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families