Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q51404

- FUMC1_PSEAE

UniProt

Q51404 - FUMC1_PSEAE

Protein

Fumarate hydratase class II 1

Gene

fumC1

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the reversible addition of water to fumarate to give L-malate.By similarity

    Catalytic activityi

    (S)-malate = fumarate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei182 – 1821Proton donor/acceptorBy similarity
    Active sitei312 – 3121By similarity
    Binding sitei313 – 3131SubstrateUniRule annotation
    Sitei325 – 3251Important for catalytic activityBy similarity

    GO - Molecular functioni

    1. fumarate hydratase activity Source: PseudoCAP

    GO - Biological processi

    1. cellular response to iron ion starvation Source: PseudoCAP
    2. fumarate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00223; UER01007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fumarate hydratase class II 1UniRule annotation (EC:4.2.1.2UniRule annotation)
    Short name:
    Fumarase C 1UniRule annotation
    Gene namesi
    Name:fumC1UniRule annotation
    Ordered Locus Names:PA4470
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA4470.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. tricarboxylic acid cycle enzyme complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 458458Fumarate hydratase class II 1PRO_0000161299Add
    BLAST

    Proteomic databases

    PRIDEiQ51404.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi208964.PA4470.

    Structurei

    3D structure databases

    ProteinModelPortaliQ51404.
    SMRiQ51404. Positions 5-447.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni98 – 1003Substrate bindingUniRule annotation
    Regioni123 – 1264B siteUniRule annotation
    Regioni133 – 1353Substrate bindingUniRule annotation
    Regioni181 – 1822Substrate bindingUniRule annotation
    Regioni318 – 3203Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0114.
    HOGENOMiHOG000061736.
    KOiK01679.
    OMAiLNVMLPI.
    OrthoDBiEOG6V1M4M.
    PhylomeDBiQ51404.

    Family and domain databases

    Gene3Di1.10.275.10. 1 hit.
    HAMAPiMF_00743. FumaraseC.
    InterProiIPR005677. Fum_hydII.
    IPR024083. Fumarase/histidase_N.
    IPR018951. Fumarase_C_C.
    IPR020557. Fumarate_lyase_CS.
    IPR000362. Fumarate_lyase_fam.
    IPR022761. Fumarate_lyase_N.
    IPR008948. L-Aspartase-like.
    [Graphical view]
    PANTHERiPTHR11444. PTHR11444. 1 hit.
    PfamiPF10415. FumaraseC_C. 1 hit.
    PF00206. Lyase_1. 1 hit.
    [Graphical view]
    PRINTSiPR00149. FUMRATELYASE.
    SUPFAMiSSF48557. SSF48557. 1 hit.
    PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q51404-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDTRIERDS MGELAVPATA LYGAQTQRAV NNFPVSGQRM PQAFVRALLL    50
    AKAAAARANV SLQQLDAPMG EAIADTCLQL LQEDFMQHFP VDVFQTGSGT 100
    SSNMNANEVV ATLASRRLGG KVNPNDHVNC GQSSNDIIPS TIHISAALEI 150
    SERLLPALRH LEQTIQSKAG EVHAYVKTGR THLMDAMPVR MSQVLGGWAQ 200
    QVRQAGVHIE SVLPALQQLA QGGTAVGTGI NAHPRFAERF SQELNDLTGL 250
    AFRPGDDFFA LIGSQDTAVA ASGQLKTLAV TLMKLANDLR WMNSGPLAGL 300
    GEIELEALQP GSSIMPGKVN PVIPEATAMV AAQVIGNDAA IAVAGQSGNF 350
    ELNVMLPLVA DNLLHSIQLL ANVSRLLADK AIASFKVNQG KLSEALARNP 400
    ILVTALNPII GYQKAAEIAK QAYREGRPII DVALENTDLD RARLEVLLDP 450
    EKLTAGGL 458
    Length:458
    Mass (Da):48,696
    Last modified:November 1, 1996 - v1
    Checksum:i48D87C8B1C76B708
    GO

    Sequence cautioni

    The sequence AAB17389.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti156 – 1561P → S in AAB17389. (PubMed:8806672)Curated
    Sequence conflicti222 – 2221G → A in AAB17389. (PubMed:8806672)Curated
    Sequence conflicti331 – 3311A → G in AAB17389. (PubMed:8806672)Curated
    Sequence conflicti340 – 3401Missing in AAB17389. (PubMed:8806672)Curated
    Sequence conflicti362 – 3621N → T in AAB17389. (PubMed:8806672)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59458 Genomic DNA. Translation: AAB02830.1.
    U72494 Genomic DNA. Translation: AAB17389.1. Different initiation.
    AE004091 Genomic DNA. Translation: AAG07858.1.
    PIRiF83088.
    JC4982.
    RefSeqiNP_253160.1. NC_002516.2.
    WP_003112741.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG07858; AAG07858; PA4470.
    GeneIDi881033.
    KEGGipae:PA4470.
    PATRICi19843667. VBIPseAer58763_4680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U59458 Genomic DNA. Translation: AAB02830.1 .
    U72494 Genomic DNA. Translation: AAB17389.1 . Different initiation.
    AE004091 Genomic DNA. Translation: AAG07858.1 .
    PIRi F83088.
    JC4982.
    RefSeqi NP_253160.1. NC_002516.2.
    WP_003112741.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q51404.
    SMRi Q51404. Positions 5-447.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA4470.

    Proteomic databases

    PRIDEi Q51404.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG07858 ; AAG07858 ; PA4470 .
    GeneIDi 881033.
    KEGGi pae:PA4470.
    PATRICi 19843667. VBIPseAer58763_4680.

    Organism-specific databases

    PseudoCAPi PA4470.

    Phylogenomic databases

    eggNOGi COG0114.
    HOGENOMi HOG000061736.
    KOi K01679.
    OMAi LNVMLPI.
    OrthoDBi EOG6V1M4M.
    PhylomeDBi Q51404.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01007 .

    Family and domain databases

    Gene3Di 1.10.275.10. 1 hit.
    HAMAPi MF_00743. FumaraseC.
    InterProi IPR005677. Fum_hydII.
    IPR024083. Fumarase/histidase_N.
    IPR018951. Fumarase_C_C.
    IPR020557. Fumarate_lyase_CS.
    IPR000362. Fumarate_lyase_fam.
    IPR022761. Fumarate_lyase_N.
    IPR008948. L-Aspartase-like.
    [Graphical view ]
    PANTHERi PTHR11444. PTHR11444. 1 hit.
    Pfami PF10415. FumaraseC_C. 1 hit.
    PF00206. Lyase_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00149. FUMRATELYASE.
    SUPFAMi SSF48557. SSF48557. 1 hit.
    PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Hassett D.J., Klotz M.G., Howell M.L.
      Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: FRD1.
    2. "The Pseudomonas aeruginosa fumC and sodA genes belong to an iron-responsive operon."
      Polack B., Dacheux D., Delic-Attree I., Toussaint B., Vignais P.M.
      Biochem. Biophys. Res. Commun. 226:555-560(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CHA.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiFUMC1_PSEAE
    AccessioniPrimary (citable) accession number: Q51404
    Secondary accession number(s): P72168
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.By similarity

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3