Reviewed, UniProtKB/Swiss-Prot Q51390 (GLPK1_PSEAE)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 1
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase 1
    Glycerokinase 1
      Short name=GK 1

Gene names

Name:	glpK1
Synonyms:	glpK
Ordered Locus Names:	PA3582

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Glycerol kinase 1 HAMAP MF_00186		PRO_0000059476
Regions
	Nucleotide binding	415 – 419	5	ATP By similarity
Sites
	Binding site	17	1	Substrate By similarity
	Binding site	21	1	ATP By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	271	1	ATP By similarity
	Binding site	314	1	ATP; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	75	1	H → R in AAB57804. Ref.1
	Sequence conflict	104	1	A → V in AAB57804. Ref.1
	Sequence conflict	109	1	C → R in AAB57804. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q51390-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: CC63A9AF8ABCF752	FASTA	505	55,966
	10 20 30 40 50 60 MTDKHNKKYV VALDQGTTSS RAIVFDRDAN VVSQAQREFA QFYPQAGWVE HDPMEIWATQ 70 80 90 100 110 120 SSTLVEALAQ ASIEHDQVAA IGITNQRETT VVWDRHSGRP IHNAIVWQCR RSAAICAQLK 130 140 150 160 170 180 RDGLEDYIRE TTGLVTDPYF SGTKLKWILD NVEGARERAR NGDLLFGTID TWLIWKLTEG 190 200 210 220 230 240 KVHVTDYTNA SRTMLFNIHS RDWDARMLEV LDIPRSMLPE VRNSSEVYGN ARIGGVGGGE 250 260 270 280 290 300 LPIAGIAGDQ QAALFGQMCV EPGQAKNTYG TGCFLLMHTG DKAVKSTHGL LTTIACGPRG 310 320 330 340 350 360 EVGYALEGAV FNGGSTVQWL RDELKVINDS FDSEYFATKV KDSNGVYLVP AFTGLGAPYW 370 380 390 400 410 420 DPYARGAVFG LTRGVKADHL IRATLESIAY QTRDVLDAMQ RDAGERLRAL RVDGGAVANN 430 440 450 460 470 480 FLMQFQADIL GTRVERPVMR ETTALGAAYL AGLACGFWSS LDELKSKAVI ERVFEPECDE 490 500 PRREKLYAGW KKAVERTRGW DDGEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa." Schweizer H.P., Jump R., Po C. Microbiology 143:1287-1297(1997) [PubMed: 9141691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49666 Genomic DNA. Translation: AAB57804.1. AE004091 Genomic DNA. Translation: AAG06970.1.
PIR	H83196.
RefSeq	NP_252272.1.
3D structure databases
SMR	Q51390. Positions 7-502.
ModBase	Search...
Genome annotation databases
GeneID	880162.
GenomeReviews	Gene locus PA3582 in contig AE004091_GR.
KEGG	pae:PA3582.
NMPDR	fig|208964.1.peg.3582.
Organism-specific databases
PseudoCAP	PA3582.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	KPSSEVY.
Enzyme and pathway databases
BioCyc	PAER208964:PA3582-MONOMER.
BRENDA	2.7.1.30. 354.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK1_PSEAE

Accession

Primary (citable) accession number: Q51390

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 8, 2000
Last modified:	February 9, 2010
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)