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Protein

Glycerol kinase 2

Gene

glpK2

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase 2 (glpK2), Glycerol kinase 1 (glpK1)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17SubstrateUniRule annotation1
Binding sitei21ATPUniRule annotation1
Binding sitei139SubstrateUniRule annotation1
Binding sitei271ATPUniRule annotation1
Binding sitei314ATP; via carbonyl oxygenUniRule annotation1
Binding sitei318ATP; via amide nitrogenUniRule annotation1
Binding sitei333ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 19ATPUniRule annotation3
Nucleotide bindingi415 – 419ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinase 2UniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferase 2UniRule annotation
Glycerokinase 2UniRule annotation
Short name:
GK 2UniRule annotation
Gene namesi
Name:glpK2UniRule annotation
Synonyms:glpK, glpK1
Ordered Locus Names:PA3582
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3582.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000594761 – 505Glycerol kinase 2Add BLAST505

Proteomic databases

PaxDbiQ51390.
PRIDEiQ51390.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3582.

Structurei

3D structure databases

ProteinModelPortaliQ51390.
SMRiQ51390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 88Substrate bindingUniRule annotation2
Regioni249 – 250Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
InParanoidiQ51390.
KOiK00864.
OMAiHTNQWDD.
PhylomeDBiQ51390.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q51390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKHNKKYV VALDQGTTSS RAIVFDRDAN VVSQAQREFA QFYPQAGWVE
60 70 80 90 100
HDPMEIWATQ SSTLVEALAQ ASIEHDQVAA IGITNQRETT VVWDRHSGRP
110 120 130 140 150
IHNAIVWQCR RSAAICAQLK RDGLEDYIRE TTGLVTDPYF SGTKLKWILD
160 170 180 190 200
NVEGARERAR NGDLLFGTID TWLIWKLTEG KVHVTDYTNA SRTMLFNIHS
210 220 230 240 250
RDWDARMLEV LDIPRSMLPE VRNSSEVYGN ARIGGVGGGE LPIAGIAGDQ
260 270 280 290 300
QAALFGQMCV EPGQAKNTYG TGCFLLMHTG DKAVKSTHGL LTTIACGPRG
310 320 330 340 350
EVGYALEGAV FNGGSTVQWL RDELKVINDS FDSEYFATKV KDSNGVYLVP
360 370 380 390 400
AFTGLGAPYW DPYARGAVFG LTRGVKADHL IRATLESIAY QTRDVLDAMQ
410 420 430 440 450
RDAGERLRAL RVDGGAVANN FLMQFQADIL GTRVERPVMR ETTALGAAYL
460 470 480 490 500
AGLACGFWSS LDELKSKAVI ERVFEPECDE PRREKLYAGW KKAVERTRGW

DDGEL
Length:505
Mass (Da):55,966
Last modified:December 8, 2000 - v2
Checksum:iCC63A9AF8ABCF752
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75H → R in AAB57804 (PubMed:9141691).Curated1
Sequence conflicti104A → V in AAB57804 (PubMed:9141691).Curated1
Sequence conflicti109C → R in AAB57804 (PubMed:9141691).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49666 Genomic DNA. Translation: AAB57804.1.
AE004091 Genomic DNA. Translation: AAG06970.1.
PIRiH83196.
RefSeqiNP_252272.1. NC_002516.2.
WP_003113886.1. NZ_ASJY01000584.1.

Genome annotation databases

EnsemblBacteriaiAAG06970; AAG06970; PA3582.
GeneIDi880162.
KEGGipae:PA3582.
PATRICi19841781. VBIPseAer58763_3748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49666 Genomic DNA. Translation: AAB57804.1.
AE004091 Genomic DNA. Translation: AAG06970.1.
PIRiH83196.
RefSeqiNP_252272.1. NC_002516.2.
WP_003113886.1. NZ_ASJY01000584.1.

3D structure databases

ProteinModelPortaliQ51390.
SMRiQ51390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3582.

Proteomic databases

PaxDbiQ51390.
PRIDEiQ51390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06970; AAG06970; PA3582.
GeneIDi880162.
KEGGipae:PA3582.
PATRICi19841781. VBIPseAer58763_3748.

Organism-specific databases

PseudoCAPiPA3582.

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
InParanoidiQ51390.
KOiK00864.
OMAiHTNQWDD.
PhylomeDBiQ51390.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK2_PSEAE
AccessioniPrimary (citable) accession number: Q51390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 8, 2000
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.