Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerol uptake facilitator protein

Gene

glpF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Glycerol metabolism, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol uptake facilitator protein
Alternative name(s):
Glycerol diffusion facilitator
Gene namesi
Name:glpF
Ordered Locus Names:PA3581
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3581.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicBy similarity
Transmembranei9 – 3729Helical; Name=M1By similarityAdd
BLAST
Topological domaini38 – 425PeriplasmicBy similarity
Transmembranei43 – 6321Helical; Name=M2By similarityAdd
BLAST
Topological domaini64 – 663CytoplasmicBy similarity
Intramembranei67 – 704By similarity
Intramembranei71 – 8111Helical; Name=M3By similarityAdd
BLAST
Topological domaini82 – 876CytoplasmicBy similarity
Transmembranei88 – 11124Helical; Name=M4By similarityAdd
BLAST
Topological domaini112 – 14635PeriplasmicBy similarityAdd
BLAST
Transmembranei147 – 17226Helical; Name=M5By similarityAdd
BLAST
Topological domaini173 – 1808CytoplasmicBy similarity
Transmembranei181 – 19717Helical; Name=M6By similarityAdd
BLAST
Topological domaini198 – 2014PeriplasmicBy similarity
Intramembranei202 – 2054By similarity
Intramembranei206 – 21914Helical; Name=M7By similarityAdd
BLAST
Topological domaini220 – 23415PeriplasmicBy similarityAdd
BLAST
Transmembranei235 – 25723Helical; Name=M8By similarityAdd
BLAST
Topological domaini258 – 27922CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Glycerol uptake facilitator proteinPRO_0000064084Add
BLAST

Proteomic databases

PaxDbiQ51389.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3581.

Structurei

3D structure databases

ProteinModelPortaliQ51389.
SMRiQ51389. Positions 9-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi71 – 733NPA 1
Motifi206 – 2083NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8C. Bacteria.
COG0580. LUCA.
HOGENOMiHOG000288287.
InParanoidiQ51389.
KOiK02440.
OMAiAREIPYF.
PhylomeDBiQ51389.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q51389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTAAPTPSL FGQCLAEFLG TALLIFFGTG CVAALKVAGA SFGLWEISII
60 70 80 90 100
WGVGVSMAIY LSAGVSGAHL NPAVSIALWL FAGFEGRKLP FYITAQVAGA
110 120 130 140 150
FCAAALVYTL YSSLFIEFEQ AQNIVRGSQD SLALASVFST YPHPALSVGQ
160 170 180 190 200
AFLVEVVITA ILMAVIMALT DDGNGLPRGP LAPLLIGLLI AVIGSAMGPL
210 220 230 240 250
TGFAMNPARD FGPKLMTYLA GWGPIAFTGG REIPYFLVPI FAPILGACLG
260 270
AGGYRVLIAR HLPSAAAPAE AEPEKVRAS
Length:279
Mass (Da):28,913
Last modified:December 8, 2000 - v2
Checksum:i4596B21F1AB14949
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161A → S in AAB57803 (PubMed:9141691).Curated
Sequence conflicti29 – 291T → A in AAB57803 (PubMed:9141691).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49666 Genomic DNA. Translation: AAB57803.1.
AE004091 Genomic DNA. Translation: AAG06969.1.
PIRiG83196.
RefSeqiNP_252271.1. NC_002516.2.
WP_003092166.1. NZ_ASJY01000584.1.

Genome annotation databases

EnsemblBacteriaiAAG06969; AAG06969; PA3581.
GeneIDi880152.
KEGGipae:PA3581.
PATRICi19841779. VBIPseAer58763_3747.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49666 Genomic DNA. Translation: AAB57803.1.
AE004091 Genomic DNA. Translation: AAG06969.1.
PIRiG83196.
RefSeqiNP_252271.1. NC_002516.2.
WP_003092166.1. NZ_ASJY01000584.1.

3D structure databases

ProteinModelPortaliQ51389.
SMRiQ51389. Positions 9-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3581.

Proteomic databases

PaxDbiQ51389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06969; AAG06969; PA3581.
GeneIDi880152.
KEGGipae:PA3581.
PATRICi19841779. VBIPseAer58763_3747.

Organism-specific databases

PseudoCAPiPA3581.

Phylogenomic databases

eggNOGiENOG4105D8C. Bacteria.
COG0580. LUCA.
HOGENOMiHOG000288287.
InParanoidiQ51389.
KOiK02440.
OMAiAREIPYF.
PhylomeDBiQ51389.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPF_PSEAE
AccessioniPrimary (citable) accession number: Q51389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 8, 2000
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.