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Q51366 (GM4D_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GDP-mannose 4,6-dehydratase
EC=4.2.1.47
Alternative name(s):
GDP-D-mannose dehydratase
Gene names
Name:gca
Ordered Locus Names:PA5453
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O.

Cofactor

NAD.

Pathway

Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2.

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.

Sequence similarities

Belongs to the GDP-mannose 4,6-dehydratase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323GDP-mannose 4,6-dehydratase
PRO_0000201715

Sites

Active site1261 By similarity
Active site1281Nucleophile By similarity
Active site1501Nucleophile By similarity
Binding site1541NADP By similarity

Experimental info

Sequence conflict1531A → V in AAC44117. Ref.1
Sequence conflict1671S → N in AAC44117. Ref.1
Sequence conflict2161A → V in AAC44117. Ref.1
Sequence conflict2231A → G in AAC44117. Ref.1
Sequence conflict2471G → V in AAC44117. Ref.1

Secondary structure

1 ......................................................... 323
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q51366 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 6C2BCC27D00D97E0

FASTA32336,399
        10         20         30         40         50         60 
MTRSALVTGI TGQDGAYLAK LLLEKGYRVH GLVARRSSDT RWRLRELGIE GDIQYEDGDM 

        70         80         90        100        110        120 
ADACSVQRAV IKAQPQEVYN LAAQSFVGAS WNQPVTTGVV DGLGVTHLLE AIRQFSPETR 

       130        140        150        160        170        180 
FYQASTSEMF GLIQAERQDE NTPFYPRSPY GVAKLYGHWI TVNYRESFGL HASSGILFNH 

       190        200        210        220        230        240 
ESPLRGIEFV TRKVTDAVAR IKLGKQQELR LGNVDAKRDW GFAGDYVEAM WLMLQQDKAD 

       250        260        270        280        290        300 
DYVVATGVTT TVRDMCQIAF EHVGLDYRDF LKIDPAFFRP AEVDVLLGNP AKAQRVLGWK 

       310        320 
PRTSLDELIR MMVEADLRRV SRE

« Hide

References

« Hide 'large scale' references
[1]"Prevalence of gca, a gene involved in synthesis of A-band common antigen polysaccharide in Pseudomonas aeruginosa."
Currie H.L., Lightfoot J., Lam J.S.
Clin. Diagn. Lab. Immunol. 2:554-562(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: IATS O5.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U18320 Genomic DNA. Translation: AAC44117.1.
AE004091 Genomic DNA. Translation: AAG08838.1.
PIRG82964.
RefSeqNP_254140.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RPNX-ray2.15A/B/C/D1-323[»]
ProteinModelPortalQ51366.
SMRQ51366. Positions 2-323.
ModBaseSearch...

Protein-protein interaction databases

STRING208964.PA5453.

Protocols and materials databases

DNASU883089.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID883089.
KEGGpae:PA5453.
PATRIC19845789. VBIPseAer58763_5716.

Organism-specific databases

PseudoCAPPA5453.

Phylogenomic databases

eggNOGCOG1089.
HOGENOMHOG000168003.
KOK01711.
OMAFVSMAFK.
ProtClustDBCLSK869363.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-12848.
UniPathwayUPA00030.
UPA00128; UER00190.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR001509. Epimerase_deHydtase.
IPR006368. GDP_Man_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01472. gmd. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ51366.

Entry information

Entry nameGM4D_PSEAE
AccessionPrimary (citable) accession number: Q51366
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 8, 2000
Last modified: May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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