Reviewed, UniProtKB/Swiss-Prot Q51363 (NADB_PSEAE)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	PA0761

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	L-aspartate oxidase		PRO_0000184393
Regions
	Nucleotide binding	10 – 24	15	FAD Potential
Sites
	Active site	244	1	By similarity
	Active site	263	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q51363-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F2B76A500DA814A1	FASTA	538	60,005
	10 20 30 40 50 60 MSQHYQHDVL VIGSGAAGLS LALTLPEHLR IAVLSKGELS QGSTYWAQGG VAAVLDDTDT 70 80 90 100 110 120 VESHVEDTLV AGGGLCREDA VRFTVEHSRE AIQWLIEQGV PFTRDEQHNH EEGSFEYHLT 130 140 150 160 170 180 REGGHSHRRI IHAADATGAA IFNTLLAQAR RRPNIELLSQ RVAVDLITER KLGLPSKRCL 190 200 210 220 230 240 GAYVLNRESG EVDTFRARFS VLASGGASKV YLYTSNPDGN SGDGIAMAWR AGCRVGNLEF 250 260 270 280 290 300 NQFHPTCLYH PQAKSFLITE ALRGEGALLR LPNGERFMPR FDPRGELAPR DIVARAIDHE 310 320 330 340 350 360 MKRLGIDCVY LDISHKPAEF IKAHFPTVYE RCLDFGIDIT QQPIPVVPAA HYTCGGVLVD 370 380 390 400 410 420 QHGHTDVPGL YAIGETTFTG LHGANRMASN SLLECFVYAR SAAADMLQRL PGTPVPESLP 430 440 450 460 470 480 SWDASQVTDS DEDVIIAHNW DELRRFMWDY VGIVRTNKRL QRAQHRVRLL LSEIDEFYSN 490 500 510 520 530 YKVSRDLIEL RNLALVAELI IRSAMQRRES RGLHYTLDYP DLLPEARDTI LVPPIYGD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production." DeVries C.A., Hassett D.J., Flynn J.L., Ohman D.E. Gene 156:63-67(1995) [PubMed: 7737518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FRD1.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternate sigma factor, and shows evidence for autoregulation." Devries C.A., Ohman D.E. J. Bacteriol. 176:6677-6687(1994) [PubMed: 7961421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31. Strain: FRD1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U17232 Genomic DNA. Translation: AAA92356.1. AE004091 Genomic DNA. Translation: AAG04150.1. L36379 Genomic DNA. Translation: AAA64438.1.
PIR	T46863.
RefSeq	NP_249452.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	882067.
GenomeReviews	Gene locus PA0761 in contig AE004091_GR.
KEGG	pae:PA0761.
Organism-specific databases
PseudoCAP	PA0761.
CMR	Search...
Phylogenomic databases
HOGENOM	Q51363.
OMA	Q51363. GAYIWNR.
Enzyme and pathway databases
BioCyc	PAER208964:PA0761-MON.
BRENDA	1.4.3.16. 354.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_PSEAE

Accession

Primary (citable) accession number: Q51363
Secondary accession number(s): Q51412

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)