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Q51355

- BLC4_PSEAI

UniProt

Q51355 - BLC4_PSEAI

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Protein

Beta-lactamase CARB-4

Gene

carB4

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Hydrolyzes carbenicillin. Methicillin and oxacillin are weakly hydrolyzed.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid and sulbactam.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Acyl-ester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase CARB-4 (EC:3.5.2.6)
Alternative name(s):
Carbenicillinase 4
Gene namesi
Name:carB4
Encoded oniPlasmid pUD120 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 288271Beta-lactamase CARB-4PRO_0000017044Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 118Sequence Analysis

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliQ51355.
SMRiQ51355. Positions 20-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2313Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q51355-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLLVFSLL IPSMVFANSS KFQQVEQDAK VIEASLSAHI GISVLDTQTG
60 70 80 90 100
EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGEINPKSTI EIKKADLVTY
110 120 130 140 150
SPVIEKQVGQ AITLDDACFA TMTTSDNAAA NIILNALGGP ESVTDFLRQI
160 170 180 190 200
GDKETRLDRI EPELNEGKLG DLRDTTTPNA IVNTLNELLF GSTLSQDGQK
210 220 230 240 250
KLEYWMVNNQ VTGNLLRSVL PEGWNIADRS GAGGFGARSI TAVVWSEAQS
260 270 280
PIIVSIYLAQ TEASIADRND AIVKIGRSIF EVYSSQSR
Length:288
Mass (Da):31,490
Last modified:November 1, 1996 - v1
Checksum:i19C068832675FD5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14749 Genomic DNA. Translation: AAC09012.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14749 Genomic DNA. Translation: AAC09012.1 .

3D structure databases

ProteinModelPortali Q51355.
SMRi Q51355. Positions 20-285.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of CARB-4 and AER-1 carbenicillin-hydrolyzing beta-lactamases."
    Sanschagrin F., Bejaoui N., Levesque R.C.
    Antimicrob. Agents Chemother. 42:1966-1972(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: P83372.
    Transposon: Tn1413.

Entry informationi

Entry nameiBLC4_PSEAI
AccessioniPrimary (citable) accession number: Q51355
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3