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Q51355

- BLC4_PSEAI

UniProt

Q51355 - BLC4_PSEAI

Protein

Beta-lactamase CARB-4

Gene

carB4

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    • Comment

    Functioni

    Hydrolyzes carbenicillin. Methicillin and oxacillin are weakly hydrolyzed.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Enzyme regulationi

    Inhibited by clavulanic acid and sulbactam.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase CARB-4 (EC:3.5.2.6)
    Alternative name(s):
    Carbenicillinase 4
    Gene namesi
    Name:carB4
    Encoded oniPlasmid pUD120 Publication
    OrganismiPseudomonas aeruginosa
    Taxonomic identifieri287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 288271Beta-lactamase CARB-4PRO_0000017044Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi72 ↔ 118Sequence Analysis

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliQ51355.
    SMRiQ51355. Positions 20-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni229 – 2313Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q51355-1 [UniParc]FASTAAdd to Basket

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    MKLLLVFSLL IPSMVFANSS KFQQVEQDAK VIEASLSAHI GISVLDTQTG    50
    EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGEINPKSTI EIKKADLVTY 100
    SPVIEKQVGQ AITLDDACFA TMTTSDNAAA NIILNALGGP ESVTDFLRQI 150
    GDKETRLDRI EPELNEGKLG DLRDTTTPNA IVNTLNELLF GSTLSQDGQK 200
    KLEYWMVNNQ VTGNLLRSVL PEGWNIADRS GAGGFGARSI TAVVWSEAQS 250
    PIIVSIYLAQ TEASIADRND AIVKIGRSIF EVYSSQSR 288
    Length:288
    Mass (Da):31,490
    Last modified:November 1, 1996 - v1
    Checksum:i19C068832675FD5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14749 Genomic DNA. Translation: AAC09012.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14749 Genomic DNA. Translation: AAC09012.1 .

    3D structure databases

    ProteinModelPortali Q51355.
    SMRi Q51355. Positions 20-285.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of CARB-4 and AER-1 carbenicillin-hydrolyzing beta-lactamases."
      Sanschagrin F., Bejaoui N., Levesque R.C.
      Antimicrob. Agents Chemother. 42:1966-1972(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: P83372.
      Transposon: Tn1413.

    Entry informationi

    Entry nameiBLC4_PSEAI
    AccessioniPrimary (citable) accession number: Q51355
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid, Transposable element

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3