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Q51355 (BLC4_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase CARB-4

EC=3.5.2.6
Alternative name(s):
Carbenicillinase 4
Gene names
Name:carB4
Encoded onPlasmid pUD12
OrganismPseudomonas aeruginosa
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes carbenicillin. Methicillin and oxacillin are weakly hydrolyzed.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulation

Inhibited by clavulanic acid and sulbactam.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Transposable element
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 288271Beta-lactamase CARB-4
PRO_0000017044

Regions

Region229 – 2313Substrate binding By similarity

Sites

Active site651Acyl-ester intermediate By similarity

Amino acid modifications

Disulfide bond72 ↔ 118 Potential

Sequences

Sequence LengthMass (Da)Tools
Q51355 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 19C068832675FD5E

FASTA28831,490
        10         20         30         40         50         60 
MKLLLVFSLL IPSMVFANSS KFQQVEQDAK VIEASLSAHI GISVLDTQTG EYWDYNGNQR 

        70         80         90        100        110        120 
FPLTSTFKTI ACAKLLYDAE QGEINPKSTI EIKKADLVTY SPVIEKQVGQ AITLDDACFA 

       130        140        150        160        170        180 
TMTTSDNAAA NIILNALGGP ESVTDFLRQI GDKETRLDRI EPELNEGKLG DLRDTTTPNA 

       190        200        210        220        230        240 
IVNTLNELLF GSTLSQDGQK KLEYWMVNNQ VTGNLLRSVL PEGWNIADRS GAGGFGARSI 

       250        260        270        280 
TAVVWSEAQS PIIVSIYLAQ TEASIADRND AIVKIGRSIF EVYSSQSR 

« Hide

References

[1]"Structure of CARB-4 and AER-1 carbenicillin-hydrolyzing beta-lactamases."
Sanschagrin F., Bejaoui N., Levesque R.C.
Antimicrob. Agents Chemother. 42:1966-1972(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: P83372.
Transposon: Tn1413.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14749 Genomic DNA. Translation: AAC09012.1.

3D structure databases

ProteinModelPortalQ51355.
SMRQ51355. Positions 20-285.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLC4_PSEAI
AccessionPrimary (citable) accession number: Q51355
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families