Reviewed, UniProtKB/Swiss-Prot Q51342 (PUR1_PSEAE)

Names and origin

Protein names

Recommended name:
    Amidophosphoribosyltransferase
      Short name=ATase
    EC=2.4.2.14
Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
      Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	PA3108

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 501	500	Amidophosphoribosyltransferase		PRO_0000139641
Regions
	Domain	2 – 234	233	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity By similarity
	Metal binding	365	1	Magnesium By similarity
	Metal binding	366	1	Magnesium By similarity
Experimental info
	Sequence conflict	286 – 290	5	LRERP → PPRAS Ref.2
	Sequence conflict	308	1	A → R in AAA83434. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q51342-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 58A3D8AD41905794	FASTA	501	55,370
	10 20 30 40 50 60 MCGIVGIVGK SNVNQALYDA LTVLQHRGQD AAGIVTCHDD KLYLRKDNGL VRDVFQQRHM 70 80 90 100 110 120 QRLIGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH NGNLTNVEQL AKEIYESDLR 130 140 150 160 170 180 HVNTNSDSEV LLNVFAHELA VRNKLQPTEE DIFAAVSCVH DRCVGGYAVV AMITGHGIVG 190 200 210 220 230 240 FRDPNAIRPI VFGQRHTENG VEYMIASESV ALDVLGFTLI RDLAPGEAVY ITEEGKLYTR 250 260 270 280 290 300 QCAKAPKYAP CIFEHVYLAR PDSIMDGISV YKARLRMGEK LADKILRERP DHDIDVVIPI 310 320 330 340 350 360 PDTSRTAALE LANRLGVKFR EGFVKNRYIG RTFIMPGQAA RKKSVRQKLN AIELEFRGKN 370 380 390 400 410 420 VMLVDDSIVR GTTCKQIIQM AREAGAKNVY FCSAAPAVRY PNVYGIDMPS AHELIAHNRS 430 440 450 460 470 480 TEDVSKLIGA DWLVYQDLPD LIDAVGGGKI KIDHFDCAVF DGEYVTGDVN EAYLNRIEQA 490 500 RNDATKAKSQ AVSAIIDLYN D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa." Foglino M., Borne F., Bally M., Ball G., Patte J.-C. Microbiology 141:431-439(1995) [PubMed: 7704274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-501. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG06496.1. U10904 Genomic DNA. Translation: AAA83434.1.
PIR	G83256.
RefSeq	NP_251798.1.
3D structure databases
SMR	Q51342. Positions 2-487.
ModBase	Search...
Genome annotation databases
GeneID	880477.
GenomeReviews	Gene locus PA3108 in contig AE004091_GR.
KEGG	pae:PA3108.
Organism-specific databases
PseudoCAP	PA3108.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392416.
OMA	GIPFELG.
Enzyme and pathway databases
BioCyc	PAER208964:PA3108-MONOMER.
BRENDA	2.4.2.14. 354.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 2 hits. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_PSEAE

Accession

Primary (citable) accession number: Q51342
Secondary accession number(s): Q9HZB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)