Reviewed,
UniProtKB/Swiss-Prot Q51342 (PUR1_PSEAE)
Last modified
February 9, 2010.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Amidophosphoribosyltransferase Short name=ATase EC=2.4.2.14 Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferase Short name=GPATase | ||||
| Gene names |
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| Organism | Pseudomonas aeruginosa [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 287 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 501 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. |
| Cofactor | Binds 1 magnesium ion per subunit. |
| Pathway | |
| Sequence similarities | In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Domain | Glutamine amidotransferase |
| Ligand | Magnesium Metal-binding |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic processInferred from electronic annotation. Source: InterPro purine base biosynthetic processInferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 501 | 500 | Amidophosphoribosyltransferase | PRO_0000139641 | |||||
Regions | |||||||||
| Domain | 2 – 234 | 233 | Glutamine amidotransferase type-2 | ||||||
Sites | |||||||||
| Active site | 2 | 1 | For GATase activity By similarity | ||||||
| Metal binding | 365 | 1 | Magnesium By similarity | ||||||
| Metal binding | 366 | 1 | Magnesium By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 286 – 290 | 5 | LRERP → PPRAS Ref.2 | ||||||
| Sequence conflict | 308 | 1 | A → R in AAA83434. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V.Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228. |
| [2] | "A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa." Foglino M., Borne F., Bally M., Ball G., Patte J.-C. Microbiology 141:431-439(1995) [PubMed: 7704274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-501. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE004091 Genomic DNA. Translation: AAG06496.1. U10904 Genomic DNA. Translation: AAA83434.1. |
| PIR | G83256. |
| RefSeq | NP_251798.1. |
3D structure databases | |
| SMR | Q51342. Positions 2-487. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 880477. |
| GenomeReviews | Gene locus PA3108 in contig AE004091_GR. |
| KEGG | pae:PA3108. |
Organism-specific databases | |
| PseudoCAP | PA3108. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG392416. |
| OMA | GIPFELG. |
Enzyme and pathway databases | |
| BioCyc | PAER208964:PA3108-MONOMER. |
| BRENDA | 2.4.2.14. 354. |
Family and domain databases | |
| InterPro | IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view] |
| PANTHER | PTHR11907. Amd_phspho_trans. 1 hit. |
| Pfam | PF00310. GATase_2. 2 hits. PF00156. Pribosyltran. 1 hit. [Graphical view] |
| PIRSF | PIRSF000485. Amd_phspho_trans. 1 hit. |
| TIGRFAMs | TIGR01134. purF. 1 hit. |
| PROSITE | PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR1_PSEAE | ||||||||
| Accession | Primary (citable) accession number: Q51342 Secondary accession number(s): Q9HZB1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


