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Q51342

- PUR1_PSEAE

UniProt

Q51342 - PUR1_PSEAE

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Protein

Amidophosphoribosyltransferase

Gene
purF, PA3108
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Metal bindingi365 – 3651Magnesium By similarity
Metal bindingi366 – 3661Magnesium By similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:PA3108
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA3108.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 501500AmidophosphoribosyltransferasePRO_0000139641Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3108.

Structurei

3D structure databases

ProteinModelPortaliQ51342.
SMRiQ51342. Positions 2-487.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 234233Glutamine amidotransferase type-2Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
HOGENOMiHOG000033687.
KOiK00764.
OMAiWLVASES.
OrthoDBiEOG6KT2Q1.
PhylomeDBiQ51342.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q51342-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIVGIVGK SNVNQALYDA LTVLQHRGQD AAGIVTCHDD KLYLRKDNGL    50
VRDVFQQRHM QRLIGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH 100
NGNLTNVEQL AKEIYESDLR HVNTNSDSEV LLNVFAHELA VRNKLQPTEE 150
DIFAAVSCVH DRCVGGYAVV AMITGHGIVG FRDPNAIRPI VFGQRHTENG 200
VEYMIASESV ALDVLGFTLI RDLAPGEAVY ITEEGKLYTR QCAKAPKYAP 250
CIFEHVYLAR PDSIMDGISV YKARLRMGEK LADKILRERP DHDIDVVIPI 300
PDTSRTAALE LANRLGVKFR EGFVKNRYIG RTFIMPGQAA RKKSVRQKLN 350
AIELEFRGKN VMLVDDSIVR GTTCKQIIQM AREAGAKNVY FCSAAPAVRY 400
PNVYGIDMPS AHELIAHNRS TEDVSKLIGA DWLVYQDLPD LIDAVGGGKI 450
KIDHFDCAVF DGEYVTGDVN EAYLNRIEQA RNDATKAKSQ AVSAIIDLYN 500
D 501
Length:501
Mass (Da):55,370
Last modified:January 23, 2007 - v3
Checksum:i58A3D8AD41905794
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti286 – 2905LRERP → PPRAS1 Publication
Sequence conflicti308 – 3081A → R in AAA83434. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG06496.1.
U10904 Genomic DNA. Translation: AAA83434.1.
PIRiG83256.
RefSeqiNP_251798.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06496; AAG06496; PA3108.
GeneIDi880477.
KEGGipae:PA3108.
PATRICi19840789. VBIPseAer58763_3260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG06496.1 .
U10904 Genomic DNA. Translation: AAA83434.1 .
PIRi G83256.
RefSeqi NP_251798.1. NC_002516.2.

3D structure databases

ProteinModelPortali Q51342.
SMRi Q51342. Positions 2-487.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA3108.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06496 ; AAG06496 ; PA3108 .
GeneIDi 880477.
KEGGi pae:PA3108.
PATRICi 19840789. VBIPseAer58763_3260.

Organism-specific databases

PseudoCAPi PA3108.

Phylogenomic databases

eggNOGi COG0034.
HOGENOMi HOG000033687.
KOi K00764.
OMAi WLVASES.
OrthoDBi EOG6KT2Q1.
PhylomeDBi Q51342.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa."
    Foglino M., Borne F., Bally M., Ball G., Patte J.-C.
    Microbiology 141:431-439(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-501.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiPUR1_PSEAE
AccessioniPrimary (citable) accession number: Q51342
Secondary accession number(s): Q9HZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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