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Q51342

- PUR1_PSEAE

UniProt

Q51342 - PUR1_PSEAE

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Metal bindingi365 – 3651MagnesiumBy similarity
    Metal bindingi366 – 3661MagnesiumBy similarity

    GO - Molecular functioni

    1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    Ordered Locus Names:PA3108
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3108.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 501500AmidophosphoribosyltransferasePRO_0000139641Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA3108.

    Structurei

    3D structure databases

    ProteinModelPortaliQ51342.
    SMRiQ51342. Positions 2-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 234233Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0034.
    HOGENOMiHOG000033687.
    KOiK00764.
    OMAiWLVASES.
    OrthoDBiEOG6KT2Q1.
    PhylomeDBiQ51342.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q51342-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIVGIVGK SNVNQALYDA LTVLQHRGQD AAGIVTCHDD KLYLRKDNGL    50
    VRDVFQQRHM QRLIGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH 100
    NGNLTNVEQL AKEIYESDLR HVNTNSDSEV LLNVFAHELA VRNKLQPTEE 150
    DIFAAVSCVH DRCVGGYAVV AMITGHGIVG FRDPNAIRPI VFGQRHTENG 200
    VEYMIASESV ALDVLGFTLI RDLAPGEAVY ITEEGKLYTR QCAKAPKYAP 250
    CIFEHVYLAR PDSIMDGISV YKARLRMGEK LADKILRERP DHDIDVVIPI 300
    PDTSRTAALE LANRLGVKFR EGFVKNRYIG RTFIMPGQAA RKKSVRQKLN 350
    AIELEFRGKN VMLVDDSIVR GTTCKQIIQM AREAGAKNVY FCSAAPAVRY 400
    PNVYGIDMPS AHELIAHNRS TEDVSKLIGA DWLVYQDLPD LIDAVGGGKI 450
    KIDHFDCAVF DGEYVTGDVN EAYLNRIEQA RNDATKAKSQ AVSAIIDLYN 500
    D 501
    Length:501
    Mass (Da):55,370
    Last modified:January 23, 2007 - v3
    Checksum:i58A3D8AD41905794
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti286 – 2905LRERP → PPRAS(PubMed:7704274)Curated
    Sequence conflicti308 – 3081A → R in AAA83434. (PubMed:7704274)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06496.1.
    U10904 Genomic DNA. Translation: AAA83434.1.
    PIRiG83256.
    RefSeqiNP_251798.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06496; AAG06496; PA3108.
    GeneIDi880477.
    KEGGipae:PA3108.
    PATRICi19840789. VBIPseAer58763_3260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG06496.1 .
    U10904 Genomic DNA. Translation: AAA83434.1 .
    PIRi G83256.
    RefSeqi NP_251798.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q51342.
    SMRi Q51342. Positions 2-487.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3108.

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06496 ; AAG06496 ; PA3108 .
    GeneIDi 880477.
    KEGGi pae:PA3108.
    PATRICi 19840789. VBIPseAer58763_3260.

    Organism-specific databases

    PseudoCAPi PA3108.

    Phylogenomic databases

    eggNOGi COG0034.
    HOGENOMi HOG000033687.
    KOi K00764.
    OMAi WLVASES.
    OrthoDBi EOG6KT2Q1.
    PhylomeDBi Q51342.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00310. GATase_2. 2 hits.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. "A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa."
      Foglino M., Borne F., Bally M., Ball G., Patte J.-C.
      Microbiology 141:431-439(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-501.
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiPUR1_PSEAE
    AccessioniPrimary (citable) accession number: Q51342
    Secondary accession number(s): Q9HZB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3