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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophileUniRule annotation1
Metal bindingi303MagnesiumUniRule annotation1
Metal bindingi365MagnesiumUniRule annotation1
Metal bindingi366MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-3164-MONOMER
UniPathwayiUPA00074; UER00124

Protein family/group databases

MEROPSiC44.001

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:PA3108
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3108

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001396412 – 501AmidophosphoribosyltransferaseAdd BLAST500

Proteomic databases

PaxDbiQ51342
PRIDEiQ51342

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3108

Structurei

3D structure databases

ProteinModelPortaliQ51342
SMRiQ51342
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 234Glutamine amidotransferase type-2UniRule annotationAdd BLAST233

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA Bacteria
COG0034 LUCA
HOGENOMiHOG000033687
InParanoidiQ51342
KOiK00764
OMAiFRPLCLG
PhylomeDBiQ51342

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 2 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q51342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIVGK SNVNQALYDA LTVLQHRGQD AAGIVTCHDD KLYLRKDNGL
60 70 80 90 100
VRDVFQQRHM QRLIGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH
110 120 130 140 150
NGNLTNVEQL AKEIYESDLR HVNTNSDSEV LLNVFAHELA VRNKLQPTEE
160 170 180 190 200
DIFAAVSCVH DRCVGGYAVV AMITGHGIVG FRDPNAIRPI VFGQRHTENG
210 220 230 240 250
VEYMIASESV ALDVLGFTLI RDLAPGEAVY ITEEGKLYTR QCAKAPKYAP
260 270 280 290 300
CIFEHVYLAR PDSIMDGISV YKARLRMGEK LADKILRERP DHDIDVVIPI
310 320 330 340 350
PDTSRTAALE LANRLGVKFR EGFVKNRYIG RTFIMPGQAA RKKSVRQKLN
360 370 380 390 400
AIELEFRGKN VMLVDDSIVR GTTCKQIIQM AREAGAKNVY FCSAAPAVRY
410 420 430 440 450
PNVYGIDMPS AHELIAHNRS TEDVSKLIGA DWLVYQDLPD LIDAVGGGKI
460 470 480 490 500
KIDHFDCAVF DGEYVTGDVN EAYLNRIEQA RNDATKAKSQ AVSAIIDLYN

D
Length:501
Mass (Da):55,370
Last modified:January 23, 2007 - v3
Checksum:i58A3D8AD41905794
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286 – 290LRERP → PPRAS (PubMed:7704274).Curated5
Sequence conflicti308A → R in AAA83434 (PubMed:7704274).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG06496.1
U10904 Genomic DNA Translation: AAA83434.1
PIRiG83256
RefSeqiNP_251798.1, NC_002516.2
WP_003091383.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG06496; AAG06496; PA3108
GeneIDi880477
KEGGipae:PA3108
PATRICifig|208964.12.peg.3260

Similar proteinsi

Entry informationi

Entry nameiPUR1_PSEAE
AccessioniPrimary (citable) accession number: Q51342
Secondary accession number(s): Q9HZB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 124 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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