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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2NucleophileUniRule annotation1
Metal bindingi303MagnesiumUniRule annotation1
Metal bindingi365MagnesiumUniRule annotation1
Metal bindingi366MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:PA3108
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3108.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001396412 – 501AmidophosphoribosyltransferaseAdd BLAST500

Proteomic databases

PaxDbiQ51342.
PRIDEiQ51342.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3108.

Structurei

3D structure databases

ProteinModelPortaliQ51342.
SMRiQ51342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 234Glutamine amidotransferase type-2UniRule annotationAdd BLAST233

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033687.
InParanoidiQ51342.
KOiK00764.
OMAiIRHFGVK.
PhylomeDBiQ51342.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q51342-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIVGK SNVNQALYDA LTVLQHRGQD AAGIVTCHDD KLYLRKDNGL
60 70 80 90 100
VRDVFQQRHM QRLIGSVGIG HVRYPTAGSS SSAEAQPFYV NSPYGITLAH
110 120 130 140 150
NGNLTNVEQL AKEIYESDLR HVNTNSDSEV LLNVFAHELA VRNKLQPTEE
160 170 180 190 200
DIFAAVSCVH DRCVGGYAVV AMITGHGIVG FRDPNAIRPI VFGQRHTENG
210 220 230 240 250
VEYMIASESV ALDVLGFTLI RDLAPGEAVY ITEEGKLYTR QCAKAPKYAP
260 270 280 290 300
CIFEHVYLAR PDSIMDGISV YKARLRMGEK LADKILRERP DHDIDVVIPI
310 320 330 340 350
PDTSRTAALE LANRLGVKFR EGFVKNRYIG RTFIMPGQAA RKKSVRQKLN
360 370 380 390 400
AIELEFRGKN VMLVDDSIVR GTTCKQIIQM AREAGAKNVY FCSAAPAVRY
410 420 430 440 450
PNVYGIDMPS AHELIAHNRS TEDVSKLIGA DWLVYQDLPD LIDAVGGGKI
460 470 480 490 500
KIDHFDCAVF DGEYVTGDVN EAYLNRIEQA RNDATKAKSQ AVSAIIDLYN

D
Length:501
Mass (Da):55,370
Last modified:January 23, 2007 - v3
Checksum:i58A3D8AD41905794
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti286 – 290LRERP → PPRAS (PubMed:7704274).Curated5
Sequence conflicti308A → R in AAA83434 (PubMed:7704274).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06496.1.
U10904 Genomic DNA. Translation: AAA83434.1.
PIRiG83256.
RefSeqiNP_251798.1. NC_002516.2.
WP_003091383.1. NZ_ASJY01000508.1.

Genome annotation databases

EnsemblBacteriaiAAG06496; AAG06496; PA3108.
GeneIDi880477.
KEGGipae:PA3108.
PATRICi19840789. VBIPseAer58763_3260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG06496.1.
U10904 Genomic DNA. Translation: AAA83434.1.
PIRiG83256.
RefSeqiNP_251798.1. NC_002516.2.
WP_003091383.1. NZ_ASJY01000508.1.

3D structure databases

ProteinModelPortaliQ51342.
SMRiQ51342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3108.

Protein family/group databases

MEROPSiC44.001.

Proteomic databases

PaxDbiQ51342.
PRIDEiQ51342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06496; AAG06496; PA3108.
GeneIDi880477.
KEGGipae:PA3108.
PATRICi19840789. VBIPseAer58763_3260.

Organism-specific databases

PseudoCAPiPA3108.

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033687.
InParanoidiQ51342.
KOiK00764.
OMAiIRHFGVK.
PhylomeDBiQ51342.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR1_PSEAE
AccessioniPrimary (citable) accession number: Q51342
Secondary accession number(s): Q9HZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.