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Q51334 (DPOL_PYRSD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase

EC=2.7.7.7
Alternative name(s):
Deep vent DNA polymerase

Cleaved into the following chain:

  1. Endonuclease PI-PspI
    EC=3.1.-.-
    Alternative name(s):
    Psp-GDB pol intein
Gene names
Name:pol
OrganismPyrococcus sp. (strain GB-D)
Taxonomic identifier69013 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length1312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.

Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Biotechnological use

Used in the PCR method because of its high thermostability and low error rate. Sold by New England Biolabs.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492DNA polymerase, 1st part
PRO_0000007330
Chain493 – 1029537Endonuclease PI-PspI
PRO_0000007331
Chain1030 – 1312283DNA polymerase, 2nd part
PRO_0000007332

Regions

Domain773 – 906134DOD-type homing endonuclease

Sequences

Sequence LengthMass (Da)Tools
Q51334 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B62518805641D26A

FASTA1,312152,854
        10         20         30         40         50         60 
MILDADYITE DGKPIIRIFK KENGEFKVEY DRNFRPYIYA LLKDDSQIDE VRKITAERHG 

        70         80         90        100        110        120 
KIVRIIDAEK VRKKFLGRPI EVWRLYFEHP QDVPAIRDKI REHSAVIDIF EYDIPFAKRY 

       130        140        150        160        170        180 
LIDKGLIPME GDEELKLLAF DIETLYHEGE EFAKGPIIMI SYADEEEAKV ITWKKIDLPY 

       190        200        210        220        230        240 
VEVVSSEREM IKRFLKVIRE KDPDVIITYN GDSFDLPYLV KRAEKLGIKL PLGRDGSEPK 

       250        260        270        280        290        300 
MQRLGDMTAV EIKGRIHFDL YHVIRRTINL PTYTLEAVYE AIFGKPKEKV YAHEIAEAWE 

       310        320        330        340        350        360 
TGKGLERVAK YSMEDAKVTY ELGREFFPME AQLSRLVGQP LWDVSRSSTG NLVEWYLLRK 

       370        380        390        400        410        420 
AYERNELAPN KPDEREYERR LRESYAGGYV KEPEKGLWEG LVSLDFRSLY PSIIITHNVS 

       430        440        450        460        470        480 
PDTLNREGCR EYDVAPEVGH KFCKDFPGFI PSLLKRLLDE RQEIKRKMKA SKDPIEKKML 

       490        500        510        520        530        540 
DYRQRAIKIL ANSILPEEWV PLIKNGKVKI FRIGDFVDGL MKANQGKVKK TGDTEVLEVA 

       550        560        570        580        590        600 
GIHAFSFDRK SKKARVMAVK AVIRHRYSGN VYRIVLNSGR KITITEGHSL FVYRNGDLVE 

       610        620        630        640        650        660 
ATGEDVKIGD LLAVPRSVNL PEKRERLNIV ELLLNLSPEE TEDIILTIPV KGRKNFFKGM 

       670        680        690        700        710        720 
LRTLRWIFGE EKRVRTASRY LRHLENLGYI RLRKIGYDII DKEGLEKYRT LYEKLVDVVR 

       730        740        750        760        770        780 
YNGNKREYLV EFNAVRDVIS LMPEEELKEW RIGTRNGFRM GTFVDIDEDF AKLLGYYVSE 

       790        800        810        820        830        840 
GSARKWKNQT GGWSYTVRLY NENDEVLDDM EHLAKKFFGK VKRGKNYVEI PKKMAYIIFE 

       850        860        870        880        890        900 
SLCGTLAENK RVPEVIFTSS KGVRWAFLEG YFIGDGDVHP SKRVRLSTKS ELLVNGLVLL 

       910        920        930        940        950        960 
LNSLGVSAIK LGYDSGVYRV YVNEELKFTE YRKKKNVYHS HIVPKDILKE TFGKVFQKNI 

       970        980        990       1000       1010       1020 
SYKKFRELVE NGKLDREKAK RIEWLLNGDI VLDRVVEIKR EYYDGYVYDL SVDEDENFLA 

      1030       1040       1050       1060       1070       1080 
GFGFLYAHNS YYGYYGYAKA RWYCKECAES VTAWGREYIE FVRKELEEKF GFKVLYIDTD 

      1090       1100       1110       1120       1130       1140 
GLYATIPGAK PEEIKKKALE FVDYINAKLP GLLELEYEGF YVRGFFVTKK KYALIDEEGK 

      1150       1160       1170       1180       1190       1200 
IITRGLEIVR RDWSEIAKET QAKVLEAILK HGNVEEAVKI VKEVTEKLSK YEIPPEKLVI 

      1210       1220       1230       1240       1250       1260 
YEQITRPLHE YKAIGPHVAV AKRLAARGVK VRPGMVIGYI VLRGDGPISK RAILAEEFDL 

      1270       1280       1290       1300       1310 
RKHKYDAEYY IENQVLPAVL RILEAFGYRK EDLRWQKTKQ TGLTAWLNIK KK 

« Hide

References

[1]"In vitro protein splicing of purified precursor and the identification of a branched intermediate."
Xu M.-Q., Southworth M.W., Mersha F.B., Hornstra L.J., Perler F.B.
Cell 75:1371-1377(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 493-517.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00707 Genomic DNA. Translation: AAA67130.1.
U00707 Genomic DNA. Translation: AAA67131.1.
U00707 Genomic DNA. Translation: AAA67132.1.
PIRS68593.

3D structure databases

ProteinModelPortalQ51334.
SMRQ51334. Positions 1-1310.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2619. PI-PspI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.170.16.10. 2 hits.
3.10.28.10. 2 hits.
3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR004578. DNA-dir_DNA_pol_B_pol2.
IPR023211. DNA_pol_palm_dom.
IPR021133. HEAT_type_2.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 2 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSPR00106. DNAPOLB.
PR00379. INTEIN.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 2 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR00592. pol2. 2 hits.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_PYRSD
AccessionPrimary (citable) accession number: Q51334
Secondary accession number(s): Q51335, Q51336
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries