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Q51161

- DHPS_NEIMB

UniProt

Q51161 - DHPS_NEIMB

Protein

Dihydropteroate synthase

Gene

folP

Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (08 Dec 2000)
      Previous versions | rss
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    Functioni

    DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

    Catalytic activityi

    (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

    Cofactori

    Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi25 – 251MagnesiumBy similarity
    Binding sitei33 – 331SubstrateBy similarity
    Binding sitei99 – 991SubstrateBy similarity
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei190 – 1901SubstrateBy similarity
    Binding sitei229 – 2291SubstrateBy similarity
    Binding sitei264 – 2641SubstrateBy similarity
    Binding sitei266 – 2661SubstrateBy similarity

    GO - Molecular functioni

    1. dihydropteroate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. response to antibiotic Source: UniProtKB-KW
    3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Antibiotic resistance, Folate biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciNMEN122586:GHGG-1746-MONOMER.
    UniPathwayiUPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropteroate synthase (EC:2.5.1.15)
    Short name:
    DHPS
    Alternative name(s):
    Dihydropteroate pyrophosphorylase
    Gene namesi
    Name:folP
    Synonyms:dhpS
    Ordered Locus Names:NMB1691
    OrganismiNeisseria meningitidis serogroup B (strain MC58)
    Taxonomic identifieri122586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
    ProteomesiUP000000425: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Dihydropteroate synthasePRO_0000168218Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi122586.NMB1691.

    Structurei

    3D structure databases

    ProteinModelPortaliQ51161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 276259Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni66 – 672Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DHPS family.Curated
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0294.
    KOiK00796.
    OMAiDCWISVD.
    OrthoDBiEOG67T5P5.

    Family and domain databases

    Gene3Di3.20.20.20. 1 hit.
    InterProiIPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view]
    PfamiPF00809. Pterin_bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF51717. SSF51717. 1 hit.
    TIGRFAMsiTIGR01496. DHPS. 1 hit.
    PROSITEiPS00792. DHPS_1. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q51161-1 [UniParc]FASTAAdd to Basket

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    MARHVWQAGR FEIGLDKPKI MGIVNLTPDS FSDGGVYSQN AQTALAHAEQ    50
    LLKEGADILD IGGESTRSGA DYVSPEEEWA RVEPVLAEVA GWGVPISLDT 100
    RRTVIMEKAL ALGGIDIIND VAALNDEGAV ELLARQADTG ICLMHMQGLP 150
    KTMQINPKYQ DVVGEVARYL KARSAECIAA GIAPQRIILD PGFGSGFGKP 200
    LQHNIALMRH LPELMAETGF PLLIGVSRKS TIGELTGEAN AAERVHGSVA 250
    AALASVARGA QIVRVHDVKA TADALKVWEA LGINL 285
    Length:285
    Mass (Da):30,313
    Last modified:December 8, 2000 - v2
    Checksum:iC56518F61CF98A4E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti2 – 43ARH → VGC in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti31 – 311F → L in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti36 – 361V → A in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti42 – 421Q → R in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti50 – 501Q → R in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti68 – 681S → P in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti69 – 691G → C in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti84 – 841P → S in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti96 – 961I → V in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti102 – 1021R → H in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti105 – 1051I → V in strain: 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti125 – 1251N → T in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti130 – 1301V → L in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti135 – 1351R → C in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti137 – 1371A → T in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti147 – 1471Q → R in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti151 – 1511K → E in strain: BT490 / Serogroup B / Serotype 15 and 952 / Serogroup B / Serotype NT.
    Natural varianti152 – 1521T → N in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti172 – 1721A → T in strain: 952 / Serogroup B / Serotype NT.
    Natural varianti174 – 1741S → A in strain: BT490 / Serogroup B / Serotype 15 and 3976 / Serogroup B / Serotype NT.
    Natural varianti175 – 1784AECI → ETCV in strain: 952 / Serogroup B / Serotype NT.
    Natural varianti188 – 1881I → T in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti194 – 1952Missing in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti196 – 1961G → C in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti200 – 2001P → T in strain: 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti206 – 2061A → T in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti208 – 2081M → V in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti220 – 2201F → Y in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti230 – 2301S → R in strain: 952 / Serogroup B / Serotype NT and BT490 / Serogroup B / Serotype 15.
    Natural varianti231 – 2311T → M in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti232 – 2321I → V in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti239 – 2391A → S in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti239 – 2391A → T in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti240 – 2401N → D in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
    Natural varianti243 – 2431E → A in strain: BT490 / Serogroup B / Serotype 15 and 3976 / Serogroup B / Serotype NT.
    Natural varianti245 – 2451V → G in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti253 – 2531L → V in strain: BT490 / Serogroup B / Serotype 15.
    Natural varianti255 – 2551S → A in strain: BT490 / Serogroup B / Serotype 15 and 3976 / Serogroup B / Serotype NT.
    Natural varianti261 – 2611Q → K in strain: 3976 / Serogroup B / Serotype NT.
    Natural varianti277 – 2771V → A in strain: 3976 / Serogroup B / Serotype NT.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68062 Genomic DNA. Translation: CAA48199.1.
    X68066 Genomic DNA. Translation: CAA48203.1.
    X68069 Genomic DNA. Translation: CAA48206.1.
    X87405 Genomic DNA. Translation: CAA60855.1.
    AE002098 Genomic DNA. Translation: AAF42039.1.
    PIRiA57423.
    S25611.
    S65832.
    S65839.
    RefSeqiNP_274695.1. NC_003112.2.
    WP_002224358.1. NC_003112.2.

    Genome annotation databases

    EnsemblBacteriaiAAF42039; AAF42039; NMB1691.
    GeneIDi903415.
    KEGGinme:NMB1691.
    PATRICi20359335. VBINeiMen85645_2174.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68062 Genomic DNA. Translation: CAA48199.1 .
    X68066 Genomic DNA. Translation: CAA48203.1 .
    X68069 Genomic DNA. Translation: CAA48206.1 .
    X87405 Genomic DNA. Translation: CAA60855.1 .
    AE002098 Genomic DNA. Translation: AAF42039.1 .
    PIRi A57423.
    S25611.
    S65832.
    S65839.
    RefSeqi NP_274695.1. NC_003112.2.
    WP_002224358.1. NC_003112.2.

    3D structure databases

    ProteinModelPortali Q51161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 122586.NMB1691.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF42039 ; AAF42039 ; NMB1691 .
    GeneIDi 903415.
    KEGGi nme:NMB1691.
    PATRICi 20359335. VBINeiMen85645_2174.

    Phylogenomic databases

    eggNOGi COG0294.
    KOi K00796.
    OMAi DCWISVD.
    OrthoDBi EOG67T5P5.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00156 .
    BioCyci NMEN122586:GHGG-1746-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.20. 1 hit.
    InterProi IPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view ]
    Pfami PF00809. Pterin_bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51717. SSF51717. 1 hit.
    TIGRFAMsi TIGR01496. DHPS. 1 hit.
    PROSITEi PS00792. DHPS_1. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transformational exchanges in the dihydropteroate synthase gene of Neisseria meningitidis: a novel mechanism for acquisition of sulfonamide resistance."
      Raadstroem P., Fermer C., Kristiansen B.-E., Jenkins A., Skoeld O., Swedberg G.
      J. Bacteriol. 174:6386-6393(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 952 / Serogroup B / Serotype NT, BT490 / Serogroup B / Serotype 15 and MO035 / Serogroup B / Serotype 15.
    2. "Sulfonamide resistance in Neisseria meningitidis as defined by site-directed mutagenesis could have its origin in other species."
      Fermer C., Kristiansen B.E., Skoeld O., Swedberg G.
      J. Bacteriol. 177:4669-4675(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 3976 / Serogroup B / Serotype NT.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MC58.

    Entry informationi

    Entry nameiDHPS_NEIMB
    AccessioniPrimary (citable) accession number: Q51161
    Secondary accession number(s): Q51157
    , Q51158, Q51159, Q51160, Q57214, Q60395
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 8, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3