Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q51161 (DHPS_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydropteroate synthase

Short name=DHPS
EC=2.5.1.15
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene names
Name:folP
Synonyms:dhpS
Ordered Locus Names:NMB1691
OrganismNeisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]
Taxonomic identifier122586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

Catalytic activity

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactor

Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the DHPS family.

Contains 1 pterin-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Dihydropteroate synthase
PRO_0000168218

Regions

Domain18 – 276259Pterin-binding
Region66 – 672Substrate binding By similarity

Sites

Metal binding251Magnesium By similarity
Binding site331Substrate By similarity
Binding site991Substrate By similarity
Binding site1191Substrate By similarity
Binding site1901Substrate By similarity
Binding site2291Substrate By similarity
Binding site2641Substrate By similarity
Binding site2661Substrate By similarity

Natural variations

Natural variant2 – 43ARH → VGC in strain: 3976 / Serogroup B / Serotype NT.
Natural variant311F → L in strain: 3976 / Serogroup B / Serotype NT.
Natural variant361V → A in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant421Q → R in strain: 3976 / Serogroup B / Serotype NT.
Natural variant501Q → R in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant681S → P in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant691G → C in strain: BT490 / Serogroup B / Serotype 15.
Natural variant841P → S in strain: 3976 / Serogroup B / Serotype NT.
Natural variant961I → V in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant1021R → H in strain: 3976 / Serogroup B / Serotype NT.
Natural variant1051I → V in strain: 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant1251N → T in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant1301V → L in strain: 3976 / Serogroup B / Serotype NT.
Natural variant1351R → C in strain: 3976 / Serogroup B / Serotype NT.
Natural variant1371A → T in strain: BT490 / Serogroup B / Serotype 15.
Natural variant1471Q → R in strain: BT490 / Serogroup B / Serotype 15.
Natural variant1511K → E in strain: BT490 / Serogroup B / Serotype 15 and 952 / Serogroup B / Serotype NT.
Natural variant1521T → N in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant1721A → T in strain: 952 / Serogroup B / Serotype NT.
Natural variant1741S → A in strain: BT490 / Serogroup B / Serotype 15 and 3976 / Serogroup B / Serotype NT.
Natural variant175 – 1784AECI → ETCV in strain: 952 / Serogroup B / Serotype NT.
Natural variant1881I → T in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant194 – 1952Missing in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant1961G → C in strain: 3976 / Serogroup B / Serotype NT.
Natural variant2001P → T in strain: 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant2061A → T in strain: 3976 / Serogroup B / Serotype NT.
Natural variant2081M → V in strain: BT490 / Serogroup B / Serotype 15.
Natural variant2201F → Y in strain: 3976 / Serogroup B / Serotype NT.
Natural variant2301S → R in strain: 952 / Serogroup B / Serotype NT and BT490 / Serogroup B / Serotype 15.
Natural variant2311T → M in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant2321I → V in strain: BT490 / Serogroup B / Serotype 15.
Natural variant2391A → S in strain: BT490 / Serogroup B / Serotype 15.
Natural variant2391A → T in strain: 3976 / Serogroup B / Serotype NT.
Natural variant2401N → D in strain: BT490 / Serogroup B / Serotype 15, 952 / Serogroup B / Serotype NT and 3976 / Serogroup B / Serotype NT.
Natural variant2431E → A in strain: BT490 / Serogroup B / Serotype 15 and 3976 / Serogroup B / Serotype NT.
Natural variant2451V → G in strain: 3976 / Serogroup B / Serotype NT.
Natural variant2531L → V in strain: BT490 / Serogroup B / Serotype 15.
Natural variant2551S → A in strain: BT490 / Serogroup B / Serotype 15 and 3976 / Serogroup B / Serotype NT.
Natural variant2611Q → K in strain: 3976 / Serogroup B / Serotype NT.
Natural variant2771V → A in strain: 3976 / Serogroup B / Serotype NT.

Sequences

Sequence LengthMass (Da)Tools
Q51161 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: C56518F61CF98A4E

FASTA28530,313
        10         20         30         40         50         60 
MARHVWQAGR FEIGLDKPKI MGIVNLTPDS FSDGGVYSQN AQTALAHAEQ LLKEGADILD 

        70         80         90        100        110        120 
IGGESTRSGA DYVSPEEEWA RVEPVLAEVA GWGVPISLDT RRTVIMEKAL ALGGIDIIND 

       130        140        150        160        170        180 
VAALNDEGAV ELLARQADTG ICLMHMQGLP KTMQINPKYQ DVVGEVARYL KARSAECIAA 

       190        200        210        220        230        240 
GIAPQRIILD PGFGSGFGKP LQHNIALMRH LPELMAETGF PLLIGVSRKS TIGELTGEAN 

       250        260        270        280 
AAERVHGSVA AALASVARGA QIVRVHDVKA TADALKVWEA LGINL 

« Hide

References

« Hide 'large scale' references
[1]"Transformational exchanges in the dihydropteroate synthase gene of Neisseria meningitidis: a novel mechanism for acquisition of sulfonamide resistance."
Raadstroem P., Fermer C., Kristiansen B.-E., Jenkins A., Skoeld O., Swedberg G.
J. Bacteriol. 174:6386-6393(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 952 / Serogroup B / Serotype NT, BT490 / Serogroup B / Serotype 15 and MO035 / Serogroup B / Serotype 15.
[2]"Sulfonamide resistance in Neisseria meningitidis as defined by site-directed mutagenesis could have its origin in other species."
Fermer C., Kristiansen B.E., Skoeld O., Swedberg G.
J. Bacteriol. 177:4669-4675(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 3976 / Serogroup B / Serotype NT.
[3]"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58."
Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. expand/collapse author list , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C.
Science 287:1809-1815(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MC58.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68062 Genomic DNA. Translation: CAA48199.1.
X68066 Genomic DNA. Translation: CAA48203.1.
X68069 Genomic DNA. Translation: CAA48206.1.
X87405 Genomic DNA. Translation: CAA60855.1.
AE002098 Genomic DNA. Translation: AAF42039.1.
PIRA57423.
S25611.
S65832.
S65839.
RefSeqNP_274695.1. NC_003112.2.

3D structure databases

ProteinModelPortalQ51161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING122586.NMB1691.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF42039; AAF42039; NMB1691.
GeneID903415.
KEGGnme:NMB1691.
PATRIC20359335. VBINeiMen85645_2174.

Phylogenomic databases

eggNOGCOG0294.
KOK00796.
OMASIDTYHA.
OrthoDBEOG67T5P5.
ProtClustDBCLSK878184.

Enzyme and pathway databases

BioCycNMEN122586:GHGG-1746-MONOMER.
UniPathwayUPA00077; UER00156.

Family and domain databases

Gene3D3.20.20.20. 1 hit.
InterProIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMSSF51717. SSF51717. 1 hit.
TIGRFAMsTIGR01496. DHPS. 1 hit.
PROSITEPS00792. DHPS_1. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHPS_NEIMB
AccessionPrimary (citable) accession number: Q51161
Secondary accession number(s): Q51157 expand/collapse secondary AC list , Q51158, Q51159, Q51160, Q57214, Q60395
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 8, 2000
Last modified: February 19, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways