Reviewed, UniProtKB/Swiss-Prot Q51083 (T2N3_NEILA)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme NlaIII
      Short name=R.NlaIII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme NlaIII
    Endonuclease NlaIII

Gene names

Name:

nlaIIIR

Organism

Neisseria lactamica

Taxonomic identifier

486 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CATG and cleaves after G-4.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Type-2 restriction enzyme NlaIII		PRO_0000077349

Sequences

	Sequence		Length	Mass (Da)
	Q51083-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 00C933962C3ADC43	FASTA	230	26,487
	10 20 30 40 50 60 MKITKTELFL RLAKPNEQGI SRWVKTSEFA GEYKDLKLGN GGSWCRKDSP LARDYIVEFD 70 80 90 100 110 120 KGLTSGNSID AIRLNGFNQE KHFKQYIRKD IKDALKTRNC VMLGVNGKSE NTKIEIDHKD 130 140 150 160 170 180 GRKNNHRVSD IKTQKLEDFQ PLCKAANDVK RQICKTCKET NKRWSAKNIS GNPYAFYMGD 190 200 210 220 230 ENYSEELGCV GCYQYDPVEY RKSSVKRIAA EAAKYTSDYI FKKLYEEDNG

References

[1]

"Molecular cloning and expression of NlaIII restriction-modification system in E. coli." Morgan R.D., Camp R.R., Wilson G.G., Xu S.-Y. Gene 183:215-218(1996) [PubMed: 8996109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U59398 Genomic DNA. Translation: AAB41400.1.
PIR	JC5751.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.21.4. 96206.
Family and domain databases
InterPro	IPR003615. HNH_nuc. IPR007979. ICEA. [Graphical view]
Pfam	PF05315. ICEA. 1 hit. [Graphical view]
SMART	SM00507. HNHc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2N3_NEILA

Accession

Primary (citable) accession number: Q51083

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	July 7, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)