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Protein

Type-2 restriction enzyme NlaIII

Gene

nlaIIIR

Organism
Neisseria lactamica
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence CATG and cleaves after G-4.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NlaIII (EC:3.1.21.4)
Short name:
R.NlaIII
Alternative name(s):
Endonuclease NlaIII
Type II restriction enzyme NlaIII
Gene namesi
Name:nlaIIIR
OrganismiNeisseria lactamica
Taxonomic identifieri486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Type-2 restriction enzyme NlaIIIPRO_0000077349Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR003615. HNH_nuc.
IPR007979. NlaIII/ICEA1.
[Graphical view]
PfamiPF05315. ICEA. 1 hit.
[Graphical view]
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q51083-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITKTELFL RLAKPNEQGI SRWVKTSEFA GEYKDLKLGN GGSWCRKDSP
60 70 80 90 100
LARDYIVEFD KGLTSGNSID AIRLNGFNQE KHFKQYIRKD IKDALKTRNC
110 120 130 140 150
VMLGVNGKSE NTKIEIDHKD GRKNNHRVSD IKTQKLEDFQ PLCKAANDVK
160 170 180 190 200
RQICKTCKET NKRWSAKNIS GNPYAFYMGD ENYSEELGCV GCYQYDPVEY
210 220 230
RKSSVKRIAA EAAKYTSDYI FKKLYEEDNG
Length:230
Mass (Da):26,487
Last modified:November 1, 1996 - v1
Checksum:i00C933962C3ADC43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59398 Genomic DNA. Translation: AAB41400.1.
PIRiJC5751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59398 Genomic DNA. Translation: AAB41400.1.
PIRiJC5751.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR003615. HNH_nuc.
IPR007979. NlaIII/ICEA1.
[Graphical view]
PfamiPF05315. ICEA. 1 hit.
[Graphical view]
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of NlaIII restriction-modification system in E. coli."
    Morgan R.D., Camp R.R., Wilson G.G., Xu S.-Y.
    Gene 183:215-218(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Entry informationi

Entry nameiT2N3_NEILA
AccessioniPrimary (citable) accession number: Q51083
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.