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Q51083 (T2N3_NEILA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NlaIII

Short name=R.NlaIII
EC=3.1.21.4
Alternative name(s):
Endonuclease NlaIII
Type II restriction enzyme NlaIII
Gene names
Name:nlaIIIR
OrganismNeisseria lactamica
Taxonomic identifier486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CATG and cleaves after G-4.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230Type-2 restriction enzyme NlaIII
PRO_0000077349

Sequences

Sequence LengthMass (Da)Tools
Q51083 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 00C933962C3ADC43

FASTA23026,487
        10         20         30         40         50         60 
MKITKTELFL RLAKPNEQGI SRWVKTSEFA GEYKDLKLGN GGSWCRKDSP LARDYIVEFD 

        70         80         90        100        110        120 
KGLTSGNSID AIRLNGFNQE KHFKQYIRKD IKDALKTRNC VMLGVNGKSE NTKIEIDHKD 

       130        140        150        160        170        180 
GRKNNHRVSD IKTQKLEDFQ PLCKAANDVK RQICKTCKET NKRWSAKNIS GNPYAFYMGD 

       190        200        210        220        230 
ENYSEELGCV GCYQYDPVEY RKSSVKRIAA EAAKYTSDYI FKKLYEEDNG 

« Hide

References

[1]"Molecular cloning and expression of NlaIII restriction-modification system in E. coli."
Morgan R.D., Camp R.R., Wilson G.G., Xu S.-Y.
Gene 183:215-218(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 23970 / CCUG 5853 / DSM 4691 / NCDC A7515 / NCTC 10617.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59398 Genomic DNA. Translation: AAB41400.1.
PIRJC5751.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003615. HNH_nuc.
IPR007979. NlaIII/ICEA1.
[Graphical view]
PfamPF05315. ICEA. 1 hit.
[Graphical view]
SMARTSM00507. HNHc. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2N3_NEILA
AccessionPrimary (citable) accession number: Q51083
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries