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Protein

Cytosolic phospholipase A2 delta

Gene

Pla2g4d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Not arachidonic acid-specific but has linoleic acid-specific activity. May play a role in inflammation in psoriatic lesions.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei370 – 3701NucleophileBy similarity
Active sitei654 – 6541Proton acceptorBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phospholipase A2 activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483115. Hydrolysis of LPC.
R-MMU-1483166. Synthesis of PA.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 delta (EC:3.1.1.4)
Short name:
cPLA2-delta
Alternative name(s):
Phospholipase A2 group IVD
Gene namesi
Name:Pla2g4d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1925640. Pla2g4d.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Cytosolic phospholipase A2 deltaPRO_0000247024Add
BLAST

Proteomic databases

PaxDbiQ50L43.
PRIDEiQ50L43.

PTM databases

iPTMnetiQ50L43.
PhosphoSiteiQ50L43.

Expressioni

Tissue specificityi

Weakly or not expressed in most tissues. Detected in placenta of 17.5 dpc embryos.1 Publication

Gene expression databases

BgeeiENSMUSG00000070719.
CleanExiMM_PLA2G4D.
GenevisibleiQ50L43. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092252.

Structurei

3D structure databases

ProteinModelPortaliQ50L43.
SMRiQ50L43. Positions 32-814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 129112C2PROSITE-ProRule annotationAdd
BLAST
Domaini281 – 825545PLA2cPROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ50L43.
KOiK16342.
OMAiKDHSAPG.
OrthoDBiEOG091G011T.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50L43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWSGDRRVGM ESLSPERLHG HPYQEEASVF CRLTVKILEA RSLPRADLLS
60 70 80 90 100
QADPYVTVQL PTASGMKFKT QTVTNSSHPV WNETFSFLIQ SQVKNILELT
110 120 130 140 150
IYDEDVITKD DICFKVSYDV SEILPGQLLQ KTFSLNPQGP EELDVELLME
160 170 180 190 200
RTWDPPENLI TNNVLVAREL SHLDVSLDRA GNTAMAAGQD KLELELMLKG
210 220 230 240 250
SYEDTQTFFP DTAFTFSFHY MRGQDTELNG YLRGPRNSGW NSDTSVTPFN
260 270 280 290 300
VPLMSLAAGK EMTIDIPAMK APEGKLQLKT DCCPKELSVR LSYGLCPEEQ
310 320 330 340 350
AFLSRRKKVV AAALKQALQL DEDLNEDEVP VVGINAEGGG MRAMISLYGH
360 370 380 390 400
LLALQKLGLL DCVTYFSGIS GSTWTMAHLY RDPEWSQRDL EGPISHAREH
410 420 430 440 450
VAKTLLKEFL PEHLASYRQT LKLREEQGYT VTVADLWGLV LESKLHGQVT
460 470 480 490 500
DQKLSGQRAA LERGQNPLPL YLSLNVKENH LETLHFKEWV EFSPYEVGFL
510 520 530 540 550
KYGGFVPSEL FGSEFFMGRL MKRLPESQIC FLEGIWSNLF SVNLMDIWYD
560 570 580 590 600
ITYGKDSNNF PVDVRNSEKE FSGSAGTSSG VEAPWLESGT ALAQALKGFL
610 620 630 640 650
TGRPFHQRSA NFLHGLQLHR DYCNQRHFST WADCNLDDTP NQLTPQDPQL
660 670 680 690 700
CLIDAGCFMN SSCPSLFRPG RQVDLIISFN YNQSLPFKGL QQSEKYSRAR
710 720 730 740 750
GLPFPRVEPS PEDHSQPQEC YLFSDPTCPE APVVLHFPLV NDSFRDHSAP
760 770 780 790 800
GVRRSPDELK AGQVNLTGAA SPYFMYNMTY KNEDFDRLLQ LSDYNVQNNQ
810 820
GTILQALRTV LKRRASETRP LGVKT
Length:825
Mass (Da):93,042
Last modified:July 27, 2011 - v3
Checksum:i257668A34FFA841B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti222 – 2221R → G in BAE39570 (PubMed:16141072).Curated
Sequence conflicti222 – 2221R → G in BAE26807 (PubMed:16141072).Curated
Sequence conflicti482 – 4821E → G in BAE39570 (PubMed:16141072).Curated
Sequence conflicti482 – 4821E → G in BAE26807 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195276 mRNA. Translation: BAD98152.1.
AK167492 mRNA. Translation: BAE39570.1.
AK145985 mRNA. Translation: BAE26807.1.
AL844608 Genomic DNA. Translation: CAM19514.1.
CCDSiCCDS16617.1.
RefSeqiNP_001019308.1. NM_001024137.1.
UniGeneiMm.330507.

Genome annotation databases

EnsembliENSMUST00000094665; ENSMUSP00000092252; ENSMUSG00000070719.
GeneIDi78390.
KEGGimmu:78390.
UCSCiuc008lvh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195276 mRNA. Translation: BAD98152.1.
AK167492 mRNA. Translation: BAE39570.1.
AK145985 mRNA. Translation: BAE26807.1.
AL844608 Genomic DNA. Translation: CAM19514.1.
CCDSiCCDS16617.1.
RefSeqiNP_001019308.1. NM_001024137.1.
UniGeneiMm.330507.

3D structure databases

ProteinModelPortaliQ50L43.
SMRiQ50L43. Positions 32-814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000092252.

PTM databases

iPTMnetiQ50L43.
PhosphoSiteiQ50L43.

Proteomic databases

PaxDbiQ50L43.
PRIDEiQ50L43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094665; ENSMUSP00000092252; ENSMUSG00000070719.
GeneIDi78390.
KEGGimmu:78390.
UCSCiuc008lvh.1. mouse.

Organism-specific databases

CTDi283748.
MGIiMGI:1925640. Pla2g4d.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ50L43.
KOiK16342.
OMAiKDHSAPG.
OrthoDBiEOG091G011T.
TreeFamiTF325228.

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483115. Hydrolysis of LPC.
R-MMU-1483166. Synthesis of PA.

Miscellaneous databases

PROiQ50L43.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070719.
CleanExiMM_PLA2G4D.
GenevisibleiQ50L43. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA24D_MOUSE
AccessioniPrimary (citable) accession number: Q50L43
Secondary accession number(s): A2AQJ1, Q3TJC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.