Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytosolic phospholipase A2 epsilon

Gene

Pla2g4e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei420 – 4201NucleophileBy similarity
Active sitei708 – 7081Proton acceptorBy similarity

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phospholipase A2 activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1483115. Hydrolysis of LPC.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 epsilon (EC:3.1.1.4)
Short name:
cPLA2-epsilon
Alternative name(s):
Phospholipase A2 group IVE
Gene namesi
Name:Pla2g4e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919144. Pla2g4e.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • lysosomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Cytosolic phospholipase A2 epsilonPRO_0000247026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei808 – 8081PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ50L42.
PaxDbiQ50L42.
PRIDEiQ50L42.

PTM databases

iPTMnetiQ50L42.
PhosphoSiteiQ50L42.

Expressioni

Tissue specificityi

Predominantly expressed in heart, skeletal muscle, testis and thyroid. Expressed at lower level in brain and stomach.1 Publication

Gene expression databases

BgeeiQ50L42.
CleanExiMM_PLA2G4E.
GenevisibleiQ50L42. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087525.

Structurei

3D structure databases

ProteinModelPortaliQ50L42.
SMRiQ50L42. Positions 82-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 168101C2PROSITE-ProRule annotationAdd
BLAST
Domaini332 – 875544PLA2cPROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ50L42.
KOiK16342.
OMAiYPKYFQP.
OrthoDBiEOG7Z0JVQ.
PhylomeDBiQ50L42.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q50L42-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSIPHSDEA DVAGMTHASE GHHGLGTSML VPKNPQGEED SKLGRNCSGF
60 70 80 90 100
EDAQDPQTAV PSSPLLSMAS CSSQEGSSPC HLLTVRIIGM KNVRQADILS
110 120 130 140 150
QTDCFVTLWL PTASQKKLKT RTISNCLHPE WDESFTFQIQ TQVKNVLELS
160 170 180 190 200
VCDEDTLTQN DHLLTVLYDL SKLCLRNKTH VKFPLNPEGM EELEVEFLLE
210 220 230 240 250
ENFSSSETLI TNGVLVSRQV SCLEVHAESR RPRKRKKNKD LLVMVTDSFE
260 270 280 290 300
NTQRVPPCQE PCYPNSACFH YPKYSQPQLY AEAPKSHCNF RLCCCGTHRN
310 320 330 340 350
DPVCQPLNCL SDGQVTTLPV GENYELHMKS SPCSDTLDVR LGFSLCQEEV
360 370 380 390 400
EFVQKRKMVV AKTLSQMLQL EEGLHEDEVP IIAIMATGGG TRSMVSLYGH
410 420 430 440 450
LLGLQKLNFL DASTYITGLS GATWTMATLY SDPEWSSKNL ETVVFEARRH
460 470 480 490 500
VVKDKMPALF PDQLYKWRED LQKHSQEGYK TTFTDFWGKL IEYSLGDKKN
510 520 530 540 550
ECKLSDQRAA LCRGQNPLPI YLTINVKDDV SNQDFREWFE FSPYEVGMQK
560 570 580 590 600
YGAFIPSELF GSEFFMGRLM KRIPEPEMCY MLGLWSSIFS LNLLDAWNLS
610 620 630 640 650
HTSEEFFYRW TRERLHDIED DPILPEIPRC DDNPLETTVV IPTTWLSNTF
660 670 680 690 700
REILTRRPFV SEFHNFLYGM QLHTDYLQNR QFSMWKDTVL DTFPNQLTQF
710 720 730 740 750
AKHLNLLDTA FFVNSSYAPL LRPERKVDLI IHLNYCAGSQ TKPLKQTCEY
760 770 780 790 800
CTEQKIPFPS FSILEDDNSL KECYVMENPQ EPDAPIVAYF PLISDTFQKY
810 820 830 840 850
KAPGVERSPD ELELGQLNIY GPKSPYATKE LTYTEAAFDK LVKLSEYNIL
860 870
NNRDKLIQAL RLAMEKKRMR SQCPS
Length:875
Mass (Da):100,157
Last modified:June 7, 2005 - v1
Checksum:i74A628C1E1A2CDEA
GO
Isoform 2 (identifier: Q50L42-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
     584-599: LWSSIFSLNLLDAWNL → DSLRYSAPERARPAFD
     600-875: Missing.

Note: No experimental confirmation available.
Show »
Length:356
Mass (Da):40,710
Checksum:i31F95C4907291D4E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti513 – 5131R → K in BAC33531 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 243243Missing in isoform 2. 1 PublicationVSP_019884Add
BLAST
Alternative sequencei584 – 59916LWSSI…DAWNL → DSLRYSAPERARPAFD in isoform 2. 1 PublicationVSP_019885Add
BLAST
Alternative sequencei600 – 875276Missing in isoform 2. 1 PublicationVSP_019886Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195277 mRNA. Translation: BAD98153.1.
AK049063 mRNA. Translation: BAC33531.1.
CCDSiCCDS16616.1. [Q50L42-1]
RefSeqiNP_808513.2. NM_177845.4. [Q50L42-1]
UniGeneiMm.330505.

Genome annotation databases

EnsembliENSMUST00000090071; ENSMUSP00000087525; ENSMUSG00000050211. [Q50L42-1]
GeneIDi329502.
KEGGimmu:329502.
UCSCiuc008lve.1. mouse. [Q50L42-1]
uc008lvg.1. mouse. [Q50L42-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195277 mRNA. Translation: BAD98153.1.
AK049063 mRNA. Translation: BAC33531.1.
CCDSiCCDS16616.1. [Q50L42-1]
RefSeqiNP_808513.2. NM_177845.4. [Q50L42-1]
UniGeneiMm.330505.

3D structure databases

ProteinModelPortaliQ50L42.
SMRiQ50L42. Positions 82-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087525.

PTM databases

iPTMnetiQ50L42.
PhosphoSiteiQ50L42.

Proteomic databases

MaxQBiQ50L42.
PaxDbiQ50L42.
PRIDEiQ50L42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090071; ENSMUSP00000087525; ENSMUSG00000050211. [Q50L42-1]
GeneIDi329502.
KEGGimmu:329502.
UCSCiuc008lve.1. mouse. [Q50L42-1]
uc008lvg.1. mouse. [Q50L42-2]

Organism-specific databases

CTDi123745.
MGIiMGI:1919144. Pla2g4e.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ50L42.
KOiK16342.
OMAiYPKYFQP.
OrthoDBiEOG7Z0JVQ.
PhylomeDBiQ50L42.
TreeFamiTF325228.

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1483115. Hydrolysis of LPC.

Miscellaneous databases

NextBioi398788.
PROiQ50L42.
SOURCEiSearch...

Gene expression databases

BgeeiQ50L42.
CleanExiMM_PLA2G4E.
GenevisibleiQ50L42. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel cytosolic phospholipase A(2)s, murine cPLA(2)delta, epsilon, and zeta, which form a gene cluster with cPLA(2)beta."
    Ohto T., Uozumi N., Hirabayashi T., Shimizu T.
    J. Biol. Chem. 280:24576-24583(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-808, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiPA24E_MOUSE
AccessioniPrimary (citable) accession number: Q50L42
Secondary accession number(s): Q8BX44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 7, 2005
Last modified: February 17, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.