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Protein

Cytosolic phospholipase A2 epsilon

Gene

Pla2g4e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei420NucleophileBy similarity1
Active sitei708Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482788 Acyl chain remodelling of PC
R-MMU-1482801 Acyl chain remodelling of PS
R-MMU-1482839 Acyl chain remodelling of PE
R-MMU-1482922 Acyl chain remodelling of PI
R-MMU-1483115 Hydrolysis of LPC

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 epsilon (EC:3.1.1.4)
Short name:
cPLA2-epsilon
Alternative name(s):
Phospholipase A2 group IVE
Gene namesi
Name:Pla2g4e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1919144 Pla2g4e

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470261 – 875Cytosolic phospholipase A2 epsilonAdd BLAST875

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei808PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ50L42
PRIDEiQ50L42

PTM databases

iPTMnetiQ50L42
PhosphoSitePlusiQ50L42
SwissPalmiQ50L42

Expressioni

Tissue specificityi

Predominantly expressed in heart, skeletal muscle, testis and thyroid. Expressed at lower level in brain and stomach.1 Publication

Gene expression databases

BgeeiENSMUSG00000050211
CleanExiMM_PLA2G4E
GenevisibleiQ50L42 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087525

Structurei

3D structure databases

ProteinModelPortaliQ50L42
SMRiQ50L42
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 168C2PROSITE-ProRule annotationAdd BLAST101
Domaini332 – 875PLA2cPROSITE-ProRule annotationAdd BLAST544

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
KOG1325 Eukaryota
ENOG410XR72 LUCA
GeneTreeiENSGT00550000074489
HOGENOMiHOG000231788
HOVERGENiHBG080412
InParanoidiQ50L42
KOiK16342
OMAiYPKYFQP
OrthoDBiEOG091G01HN
PhylomeDBiQ50L42
TreeFamiTF325228

Family and domain databases

Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF01735 PLA2_B, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS51210 PLA2C, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q50L42-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSIPHSDEA DVAGMTHASE GHHGLGTSML VPKNPQGEED SKLGRNCSGF
60 70 80 90 100
EDAQDPQTAV PSSPLLSMAS CSSQEGSSPC HLLTVRIIGM KNVRQADILS
110 120 130 140 150
QTDCFVTLWL PTASQKKLKT RTISNCLHPE WDESFTFQIQ TQVKNVLELS
160 170 180 190 200
VCDEDTLTQN DHLLTVLYDL SKLCLRNKTH VKFPLNPEGM EELEVEFLLE
210 220 230 240 250
ENFSSSETLI TNGVLVSRQV SCLEVHAESR RPRKRKKNKD LLVMVTDSFE
260 270 280 290 300
NTQRVPPCQE PCYPNSACFH YPKYSQPQLY AEAPKSHCNF RLCCCGTHRN
310 320 330 340 350
DPVCQPLNCL SDGQVTTLPV GENYELHMKS SPCSDTLDVR LGFSLCQEEV
360 370 380 390 400
EFVQKRKMVV AKTLSQMLQL EEGLHEDEVP IIAIMATGGG TRSMVSLYGH
410 420 430 440 450
LLGLQKLNFL DASTYITGLS GATWTMATLY SDPEWSSKNL ETVVFEARRH
460 470 480 490 500
VVKDKMPALF PDQLYKWRED LQKHSQEGYK TTFTDFWGKL IEYSLGDKKN
510 520 530 540 550
ECKLSDQRAA LCRGQNPLPI YLTINVKDDV SNQDFREWFE FSPYEVGMQK
560 570 580 590 600
YGAFIPSELF GSEFFMGRLM KRIPEPEMCY MLGLWSSIFS LNLLDAWNLS
610 620 630 640 650
HTSEEFFYRW TRERLHDIED DPILPEIPRC DDNPLETTVV IPTTWLSNTF
660 670 680 690 700
REILTRRPFV SEFHNFLYGM QLHTDYLQNR QFSMWKDTVL DTFPNQLTQF
710 720 730 740 750
AKHLNLLDTA FFVNSSYAPL LRPERKVDLI IHLNYCAGSQ TKPLKQTCEY
760 770 780 790 800
CTEQKIPFPS FSILEDDNSL KECYVMENPQ EPDAPIVAYF PLISDTFQKY
810 820 830 840 850
KAPGVERSPD ELELGQLNIY GPKSPYATKE LTYTEAAFDK LVKLSEYNIL
860 870
NNRDKLIQAL RLAMEKKRMR SQCPS
Length:875
Mass (Da):100,157
Last modified:June 7, 2005 - v1
Checksum:i74A628C1E1A2CDEA
GO
Isoform 2 (identifier: Q50L42-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-243: Missing.
     584-599: LWSSIFSLNLLDAWNL → DSLRYSAPERARPAFD
     600-875: Missing.

Note: No experimental confirmation available.
Show »
Length:356
Mass (Da):40,710
Checksum:i31F95C4907291D4E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti513R → K in BAC33531 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0198841 – 243Missing in isoform 2. 1 PublicationAdd BLAST243
Alternative sequenceiVSP_019885584 – 599LWSSI…DAWNL → DSLRYSAPERARPAFD in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_019886600 – 875Missing in isoform 2. 1 PublicationAdd BLAST276

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195277 mRNA Translation: BAD98153.1
AK049063 mRNA Translation: BAC33531.1
CCDSiCCDS16616.1 [Q50L42-1]
RefSeqiNP_808513.2, NM_177845.4 [Q50L42-1]
UniGeneiMm.330505

Genome annotation databases

EnsembliENSMUST00000090071; ENSMUSP00000087525; ENSMUSG00000050211 [Q50L42-1]
GeneIDi329502
KEGGimmu:329502
UCSCiuc008lve.1 mouse [Q50L42-1]
uc008lvg.1 mouse [Q50L42-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPA24E_MOUSE
AccessioniPrimary (citable) accession number: Q50L42
Secondary accession number(s): Q8BX44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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