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Protein

Cytosolic phospholipase A2 zeta

Gene

Pla2g4f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei393NucleophileBy similarity1
Active sitei685Proton acceptorBy similarity1

GO - Molecular functioni

  • calcium-dependent phospholipase A2 activity Source: MGI
  • lysophospholipase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • phospholipase A2 activity Source: MGI

GO - Biological processi

  • arachidonic acid secretion Source: MGI
  • cellular response to antibiotic Source: MGI
  • cellular response to organic cyclic compound Source: MGI
  • fatty acid transport Source: MGI
  • phospholipid catabolic process Source: InterPro
  • prostaglandin biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483115. Hydrolysis of LPC.

Chemistry databases

SwissLipidsiSLP:000001085.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 zeta (EC:3.1.1.4)
Short name:
cPLA2-zeta
Alternative name(s):
Phospholipase A2 group IVF
Gene namesi
Name:Pla2g4f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2685493. Pla2g4f.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • lysosomal membrane Source: UniProtKB-SubCell
  • ruffle membrane Source: MGI
  • vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002470281 – 855Cytosolic phospholipase A2 zetaAdd BLAST855

Proteomic databases

MaxQBiQ50L41.
PaxDbiQ50L41.
PRIDEiQ50L41.

PTM databases

PhosphoSitePlusiQ50L41.

Expressioni

Tissue specificityi

Strongly expressed in thyroid, expressed at intermediate level in stomach and at very low level in large intestine and prostate.1 Publication

Gene expression databases

BgeeiENSMUSG00000046971.
CleanExiMM_PLA2G4F.
GenevisibleiQ50L41. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062607.

Structurei

3D structure databases

ProteinModelPortaliQ50L41.
SMRiQ50L41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 133C2PROSITE-ProRule annotationAdd BLAST103
Domaini304 – 855PLA2cPROSITE-ProRule annotationAdd BLAST552

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ50L41.
KOiK16342.
OMAiGMWGSAF.
OrthoDBiEOG091G01HN.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50L41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPWTLQPKWL AGKGLPLLGA ILLRKTEKSE PQWKHRRQET HPYYDLQVKV
60 70 80 90 100
LRARNIQHTD KLSKADCYVR LWLPTASVSP SQTRTVVNSS DPEWNETFPY
110 120 130 140 150
QIHGAVKNVL ELALYDEDVL DSDNVFSILF DTSTLQLGQP CTKNFTRQQD
160 170 180 190 200
PKELEVEFTL EKSQTPASEV VTNGVLVAHP CLRIQGTVTG DKTASLGELG
210 220 230 240 250
SRQIQLAVPG AYEKPQPLQP TSEPGLPVNF TFHVNPVLSP KLHIKLQEQL
260 270 280 290 300
QVFHSGPSDE LEAQTSKMDK ASILLSSLPL NEELTKLVDL EEGQQVSLRM
310 320 330 340 350
KADMSSSGDL DLRLGFDLCD GEQEFLDKRK QVASKALQRV MGLSEALHCD
360 370 380 390 400
QVPVVAVLGS GGGTRAMTSL YGSLAGLQEL GLLDAVTYLS GVSGSSWCIS
410 420 430 440 450
TLYRDPSWSQ KALQGPIKYA SERVCSSKIG MLSPKQFEYY SREKRAWESR
460 470 480 490 500
GHSMSFTDLW GLIIEYFLNQ EENPAKLSDQ QETVSQGQNP YPIYASINVH
510 520 530 540 550
KNISGDDFAE WCEFTPYEVG FPKYGAYVPT ELFGSEFFMG RLLHFWPEPR
560 570 580 590 600
ICYLQGMWGS AFAASLYEIF LKLGGLSLSF LDWHRGSVSV TDDWPKLRKQ
610 620 630 640 650
DPTRLPTRLF TPMSSFSQAV LDIFTSRITC AQTFNFTRGL CMYKDYTARK
660 670 680 690 700
DFVVSEDAWH SHNYGYPDAC PNQLTPMKDF LSLVDGGFAI NSPFPLVLQP
710 720 730 740 750
QRAVDLIVSF DYSLEGPFEV LQVTEKYCRD RGIPFPRIEV DPKDSEDPRE
760 770 780 790 800
CYLFAEAEDP CSPIVLHFPL VNRTFRTHLA PGVERQTAEE KAFGDFIING
810 820 830 840 850
PDTAYGMMDF TYEPKEFDRL VTLSRYNVLN NKETIRHALQ LALDRRRQAG

GRVGG
Length:855
Mass (Da):96,364
Last modified:July 27, 2011 - v3
Checksum:i4F839C857E136D6F
GO

Sequence cautioni

The sequence AAH39947 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99P → H in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti132T → M in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti234V → M in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti297S → T in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti507D → Y in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti507D → Y in AAH46400 (PubMed:15489334).Curated1
Sequence conflicti526A → V in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti526A → V in AAH46400 (PubMed:15489334).Curated1
Sequence conflicti755A → T in AAH39947 (PubMed:15489334).Curated1
Sequence conflicti755A → T in AAH46400 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195278 mRNA. Translation: BAD98154.1.
AL844608 Genomic DNA. Translation: CAM19515.1.
BC039947 mRNA. Translation: AAH39947.1. Different initiation.
BC046400 mRNA. Translation: AAH46400.1.
CCDSiCCDS16618.1.
RefSeqiNP_001019316.1. NM_001024145.2.
UniGeneiMm.24880.

Genome annotation databases

EnsembliENSMUST00000054651; ENSMUSP00000062607; ENSMUSG00000046971.
GeneIDi271844.
KEGGimmu:271844.
UCSCiuc008lvi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB195278 mRNA. Translation: BAD98154.1.
AL844608 Genomic DNA. Translation: CAM19515.1.
BC039947 mRNA. Translation: AAH39947.1. Different initiation.
BC046400 mRNA. Translation: AAH46400.1.
CCDSiCCDS16618.1.
RefSeqiNP_001019316.1. NM_001024145.2.
UniGeneiMm.24880.

3D structure databases

ProteinModelPortaliQ50L41.
SMRiQ50L41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062607.

Chemistry databases

SwissLipidsiSLP:000001085.

PTM databases

PhosphoSitePlusiQ50L41.

Proteomic databases

MaxQBiQ50L41.
PaxDbiQ50L41.
PRIDEiQ50L41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054651; ENSMUSP00000062607; ENSMUSG00000046971.
GeneIDi271844.
KEGGimmu:271844.
UCSCiuc008lvi.2. mouse.

Organism-specific databases

CTDi255189.
MGIiMGI:2685493. Pla2g4f.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
KOG1325. Eukaryota.
ENOG410XR72. LUCA.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ50L41.
KOiK16342.
OMAiGMWGSAF.
OrthoDBiEOG091G01HN.
TreeFamiTF325228.

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483115. Hydrolysis of LPC.

Miscellaneous databases

PROiQ50L41.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046971.
CleanExiMM_PLA2G4F.
GenevisibleiQ50L41. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA24F_MOUSE
AccessioniPrimary (citable) accession number: Q50L41
Secondary accession number(s): A2AQJ2, Q80VQ8, Q80VV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.