Q50KB1 (SEP2_EMIHU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide-isomerase-like protein EhSep2

Gene names

Name:

SEP2

Organism

Emiliania huxleyi

Taxonomic identifier

2903 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Haptophyceae › Isochrysidales › Noelaerhabdaceae › Emiliania

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Endoplasmic reticulum lumen Potential.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain.
Caution	Contains a selenocysteine rather than a cysteine at one of the conserved active site positions so does not form the disulfide bond normally required for protein disulfide isomerase activity.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Selenocysteine
Domain	Coiled coil Signal
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Non-traceable author statement Ref.1. Source: UniProtKB endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein disulfide isomerase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.1
	Chain	18 – 223	206	Protein disulfide-isomerase-like protein EhSep2 Ref.1		PRO_0000248266
Regions
	Domain	18 – 125	108	Thioredoxin
	Coiled coil	155 – 201	47	Potential
	Motif	220 – 223	4	Prevents secretion from ER Potential
Amino acid modifications
	Non-standard residue	47	1	Selenocysteine
Experimental info
	Sequence conflict	103	1	D → V AA sequence Ref.1
	Sequence conflict	107 – 108	2	ED → GA AA sequence Ref.1
	Sequence conflict	111 – 112	2	GG → AL AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q50KB1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 9744C1A547A86F4C	FASTA	223	24,564
	10 20 30 40 50 60 MALRSLTLLC AAAGASAGAI ELTPDNFDEL VLKSGKAAFI KFLAPWUGHC KKMKPDWDSL 70 80 90 100 110 120 ASTFEDSKKV LIADVDCTTG GKPLCEKYGV RGYPTIKYFN PPDEEGEDYK GGRSLDELKK 130 140 150 160 170 180 FAENELGPGC SVDLMDNCSE EQKGKLKEYI DMAPEKRTEM LETLKKELAD AESTHEALLK 190 200 210 220 ELQATYKESM DKLEKLKEES APKIKLLKAA TPAPKAEGAK DEV

References

[1]	"A novel eukaryotic selenoprotein in the haptophyte alga Emiliania huxleyi." Obata T., Shiraiwa Y. J. Biol. Chem. 280:18462-18468(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-38 AND 98-112. Strain: NIES-873.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB205027 mRNA. Translation: BAD98262.1.
3D structure databases
HSSP	HSSP built from PDB template 1X5D based on UniProtKB Q15084.
ProteinModelPortal	Q50KB1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR005788. Disulphide_isomerase. IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01126. pdi_dom. 1 hit.
PROSITE	PS00014. ER_TARGET. False negative. PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SEP2_EMIHU

Accession

Primary (citable) accession number: Q50KB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	February 26, 2008
Last modified:	March 6, 2013
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)