Reviewed, UniProtKB/Swiss-Prot Q50KA9 (NDKA_CANFA)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase A
      Short name=NDP kinase A
      Short name=NDK A
    EC=2.7.4.6
Alternative name(s):
    nm23-C1

Gene names

Name:	NME1
Synonyms:	NM23A

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity.
Subunit structure	Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with SET and PRUNE By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Differentiation Endocytosis Neurogenesis Nucleotide metabolism
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW endocytosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 152	151	Nucleoside diphosphate kinase A		PRO_0000137113
Sites
	Active site	118	1	Pros-phosphohistidine intermediate By similarity
	Binding site	12	1	ATP By similarity
	Binding site	60	1	ATP By similarity
	Binding site	88	1	ATP By similarity
	Binding site	94	1	ATP By similarity
	Binding site	105	1	ATP By similarity
	Binding site	115	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	52	1	Phosphotyrosine By similarity
	Modified residue	56	1	N6-acetyllysine By similarity
	Modified residue	94	1	Phosphothreonine By similarity
	Cross-link	100		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50KA9-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 669D444D69380FE6	FASTA	152	17,180
	10 20 30 40 50 60 MANSERTFIA IKPDGVQRSL VGEIIKRFEQ KGFRLIAMKL IQASEDLLKE HYIDLKDRPF 70 80 90 100 110 120 FAGLVKYMQS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 130 140 150 DSVESAEKEI GLWFQPEELV DYKSCAQNWI YE

References

[1]	"Molecular cloning of canine nm23 cDNAs and their expression in normal and tumor tissues." Takahashi M., Une R., Fukushima K., Fujiki M., Misumi K., Miyoshi N., Endo Y., Ohishi A., Akuzawa M. J. Vet. Med. Sci. 67:837-841(2005) [PubMed: 16141675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB207044 mRNA. Translation: BAD97837.1.
RefSeq	NP_001019808.1.
UniGene	Cfa.15094
3D structure databases
SMR	Q50KA9. Positions 2-152.
ModBase	Search...
Protein-protein interaction databases
STRING	Q50KA9.
Genome annotation databases
Ensembl	ENSCAFT00000027420; ENSCAFP00000025500; ENSCAFG00000023628; Canis familiaris. [Genome view] ENSCAFT00000036453; ENSCAFP00000031833; ENSCAFG00000023628; Canis familiaris. [Genome view]
GeneID	480558.
KEGG	cfa:480558.
Organism-specific databases
CTD	480558.
Phylogenomic databases
eggNOG	maNOG10260.
HOVERGEN	Q50KA9.
InParanoid	Q50KA9.
Enzyme and pathway databases
BRENDA	2.7.4.6. 463.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKA_CANFA

Accession

Primary (citable) accession number: Q50KA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)