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Q50KA9 (NDKA_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nucleoside diphosphate kinase A
Short name=NDK A
Short name=NDP kinase A
EC=2.7.4.6
Alternative name(s):
nm23-C1
Gene names
Name:NME1
Synonyms:NM23A
OrganismCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination By similarity.

Catalytic activity

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactor

Magnesium By similarity.

Enzyme regulation

Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity By similarity.

Subunit structure

Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with SET and PRUNE By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the NDK family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 152151Nucleoside diphosphate kinase A
PRO_0000137113

Sites

Active site1181Pros-phosphohistidine intermediate By similarity
Binding site121ATP By similarity
Binding site601ATP By similarity
Binding site881ATP By similarity
Binding site941ATP By similarity
Binding site1051ATP By similarity
Binding site1151ATP By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue521Phosphotyrosine By similarity
Modified residue561N6-acetyllysine By similarity
Modified residue941Phosphothreonine By similarity
Cross-link100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q50KA9 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 669D444D69380FE6

FASTA15217,180
        10         20         30         40         50         60 
MANSERTFIA IKPDGVQRSL VGEIIKRFEQ KGFRLIAMKL IQASEDLLKE HYIDLKDRPF 

        70         80         90        100        110        120 
FAGLVKYMQS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 

       130        140        150 
DSVESAEKEI GLWFQPEELV DYKSCAQNWI YE

« Hide

References

[1]"Molecular cloning of canine nm23 cDNAs and their expression in normal and tumor tissues."
Takahashi M., Une R., Fukushima K., Fujiki M., Misumi K., Miyoshi N., Endo Y., Ohishi A., Akuzawa M.
J. Vet. Med. Sci. 67:837-841(2005) [PubMed: 16141675] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB207044 mRNA. Translation: BAD97837.1.
RefSeqNP_001019808.1. NM_001024637.1.
UniGeneCfa.15094.

3D structure databases

ProteinModelPortalQ50KA9.
SMRQ50KA9. Positions 2-152.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ50KA9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCAFT00000036453; ENSCAFP00000031833; ENSCAFG00000023628.
GeneID480558.
KEGGcfa:480558.

Phylogenomic databases

eggNOGmaNOG10260.
GeneTreeENSGT00550000074206.
HOVERGENHBG000423.
InParanoidQ50KA9.
OrthoDBEOG4S1T8G.

Family and domain databases

InterProIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
Gene3DG3DSA:3.30.70.141. NDK. 1 hit.
KOK00940.
PANTHERPTHR11349. Nuc_diP_kinase_core. 1 hit.
PfamPF00334. NDK. 1 hit.
[Graphical view]
PRINTSPR01243. NUCDPKINASE.
SMARTSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMSSF54919. NDK. 1 hit.
PROSITEPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDKA_CANFA
AccessionPrimary (citable) accession number: Q50KA9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: June 7, 2005
Last modified: November 16, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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