Reviewed, UniProtKB/Swiss-Prot Q50KA8 (NDKB_CANFA)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase B
      Short name=NDP kinase B
      Short name=NDK B
    EC=2.7.4.6
Alternative name(s):
    nm23-C2

Gene names

Name:	NME2
Synonyms:	NM23B

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13 and CAPN8 By similarity.
Subcellular location	Cytoplasm. Cell membrane By similarity.
Tissue specificity	Ubiquitous. Ref.1
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Nucleoside diphosphate kinase B		PRO_0000250200
Regions
	Region	1 – 66	66	Interaction with AKAP13 By similarity
Sites
	Active site	118	1	Pros-phosphohistidine intermediate By similarity
	Binding site	12	1	ATP By similarity
	Binding site	60	1	ATP By similarity
	Binding site	88	1	ATP By similarity
	Binding site	94	1	ATP By similarity
	Binding site	105	1	ATP By similarity
	Binding site	115	1	ATP By similarity
Amino acid modifications
	Modified residue	12	1	N6-acetyllysine By similarity
	Modified residue	49	1	N6-acetyllysine By similarity
	Modified residue	52	1	Phosphotyrosine By similarity
	Modified residue	56	1	N6-acetyllysine By similarity
	Modified residue	85	1	N6-acetyllysine By similarity
	Modified residue	94	1	Phosphothreonine By similarity
	Modified residue	100	1	N6-acetyllysine By similarity
	Modified residue	124	1	N6-acetyllysine By similarity
	Modified residue	128	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50KA8-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 923DA489B37C19C6	FASTA	152	17,366
	10 20 30 40 50 60 MAHQERTFIA IKPDGVQRGL VGDIVKRFEQ KGFRLVAMKF LRASEDLLKE HYIDLKDRPF 70 80 90 100 110 120 YPGLVKYMHS GPVVAMVWEG LNVVKTGRMM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 130 140 150 DSVKSAEKEI SLWFKPEELV DYKSCAFDWI YE

References

[1]

"Molecular cloning of canine nm23 cDNAs and their expression in normal and tumor tissues." Takahashi M., Une R., Fukushima K., Fujiki M., Misumi K., Miyoshi N., Endo Y., Ohishi A., Akuzawa M. J. Vet. Med. Sci. 67:837-841(2005) [PubMed: 16141675] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Peripheral blood leukocyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB207045 mRNA. Translation: BAD97838.1.
RefSeq	NP_001019809.1.
UniGene	Cfa.23354
3D structure databases
SMR	Q50KA8. Positions 2-152.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000027419; ENSCAFP00000025499; ENSCAFG00000017307; Canis familiaris. [Genome view]
GeneID	480559.
KEGG	cfa:480559.
Organism-specific databases
CTD	480559.
Phylogenomic databases
HOVERGEN	Q50KA8.
Enzyme and pathway databases
BRENDA	2.7.4.6. 463.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_CANFA

Accession

Primary (citable) accession number: Q50KA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)