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Q50JE5

- ACE_MESAU

UniProt

Q50JE5 - ACE_MESAU

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Protein

Angiotensin-converting enzyme

Gene
Ace, Dcp1
Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm By similarity.

Catalytic activityi

Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactori

Binds 2 zinc ions per subunit By similarity.
Binds 3 chloride ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei237 – 2371Chloride 1 By similarity
Metal bindingi396 – 3961Zinc 1; catalytic By similarity
Active sitei397 – 39711 By similarity
Metal bindingi400 – 4001Zinc 1; catalytic By similarity
Metal bindingi424 – 4241Zinc 1; catalytic By similarity
Binding sitei535 – 5351Chloride 1 By similarity
Binding sitei797 – 7971Chloride 2 By similarity
Binding sitei835 – 8351Chloride 3 By similarity
Metal bindingi994 – 9941Zinc 2; catalytic By similarity
Active sitei995 – 99512 By similarity
Metal bindingi998 – 9981Zinc 2; catalytic By similarity
Metal bindingi1022 – 10221Zinc 2; catalytic By similarity
Binding sitei1096 – 10961Chloride 2 By similarity
Binding sitei1100 – 11001Chloride 2 By similarity
Binding sitei1133 – 11331Chloride 3 By similarity

GO - Molecular functioni

  1. carboxypeptidase activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. metallopeptidase activity Source: UniProtKB-KW
  4. peptidyl-dipeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.15.1. 3239.

Protein family/group databases

MEROPSiM02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Angiotensin-converting enzyme (EC:3.2.1.-, EC:3.4.15.1)
Short name:
ACE
Alternative name(s):
Dipeptidyl carboxypeptidase I
Kininase II
CD_antigen: CD143
Cleaved into the following chain:
Gene namesi
Name:Ace
Synonyms:Dcp1
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 12651230Extracellular Reviewed predictionAdd
BLAST
Transmembranei1266 – 128217Helical; Reviewed predictionAdd
BLAST
Topological domaini1283 – 131432Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535 By similarityAdd
BLAST
Chaini36 – 13141279Angiotensin-converting enzymePRO_0000028536Add
BLAST
Chaini36 – 12381203Angiotensin-converting enzyme, soluble formPRO_0000028537Add
BLAST
Propeptidei1239 – 131476Removed in secreted form By similarityPRO_0000028538Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi60 – 601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi80 – 801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi117 – 1171N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi163 ↔ 171 By similarity
Glycosylationi166 – 1661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi324 – 3241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi515 – 5151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi683 – 6831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi701 – 7011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi720 – 7201N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi763 ↔ 769 By similarity
Glycosylationi766 – 7661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi948 – 9481N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi963 ↔ 981 By similarity
Disulfide bondi1149 ↔ 1161 By similarity
Glycosylationi1197 – 11971N-linked (GlcNAc...) Reviewed prediction
Modified residuei1307 – 13071Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by CK2 on Ser-1307; which allows membrane retention By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Widely expressed with dominant expression in lung and kidney.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ50JE5.
SMRiQ50JE5. Positions 36-647, 651-1234.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 636601Peptidase M2 1Add
BLAST
Regioni637 – 1238602Peptidase M2 2Add
BLAST

Sequence similaritiesi

Belongs to the peptidase M2 family.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000264.

Family and domain databases

InterProiIPR001548. Peptidase_M2.
[Graphical view]
PANTHERiPTHR10514. PTHR10514. 1 hit.
PfamiPF01401. Peptidase_M2. 2 hits.
[Graphical view]
PRINTSiPR00791. PEPDIPTASEA.
PROSITEiPS00142. ZINC_PROTEASE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Somatic (identifier: Q50JE5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGAASGQRGQ GPPSPLLLLW LSLLLLLLPP SPAPALDPGL QPGNFSADEI     50
GAHLFAESYN SSAEQVIFQS TVASWAYDTN MTEENARLQE EAELIWQEFA 100
EVWGKKAKEL FDAIRQNFTD SKLRRVIETI RTLGPANLPL ARRQQYNSLQ 150
NNMNRIYSTS KVCLPNKTAT CWSLEPELTN ILASSRSYAK LLFAWESWHD 200
VVGIPLKPLY QDFTALSNEA YKQDGFSDTG AYWRSAYDSP SFEETLEHLY 250
HQLEPLYLNL HAYVRRALHR RYGDKYINLR GPIPAHLLGD MWAQSWDNIY 300
DMVVPFPNKP NLDVTNTMVQ KGWNVTHMFR VAEEFFTSMG LSPMPPEFWA 350
ESMLEKPTDG REVVCHASAW DFFNRKDFRI KQCTRITMEQ LSTVHHEMGH 400
VQYYLQYKDL TVPLRRGANP GFHEAIGDVL ALSVSTPAHL HKIGLLDRVA 450
NDLESDINYL LKMALEKIAF LPFGYLVDQW RWGVFSGHTP PSRYNFDWWY 500
FRTKYQGICP PVVRNETHFD AGAKFHIPSG TPYIRYFVSF ILQFQFHQAL 550
CKEAGHQGPL HQCDIYQSTQ AGAKLQRVLQ AGYSRPWQEV LKEMVGSDTL 600
DAQALLEYFQ PVIRWLQEQN QRNGEVLGWP EYQWRPPLPD NYPEGIDLVT 650
DETEAERFVE EYDRTARVLW NEYAEANWQY NTNITLEASK ILLQKNKKVA 700
NHTLKYGTLA KKFDVSNFQN YTIKRIIKKV QNMDRAVLPP KELEEYNQIL 750
MDMETTYSIA NVCYLNGTCL HLEPDLTNVM ATSRKYEELL WVWKSWRDKV 800
GRAILPLFPK YVELSNKIAH LNGYADGGDS WRSSYESKSL EQDLEQLYQE 850
LQPLYLNLHA YVRRSLHRHY GSQHINLDGP IPAHLLGNMW AQTWSNIYDL 900
VAPFPSAPNL DATEAMIKQG WTPRRIFKEA DDFFTSLGLL PVSEEFWNKS 950
MLEKPGDGRE VVCHASAWDF YNGKDFRIKQ CTSVNMEDLV IAHHEMGHIQ 1000
YFMQYKDLPV TFREGANPGF HEAIGDVLAL SVSTPKHLHS LNLLSSEGGG 1050
YEHDINFLMK MALDKIAFIP FSYLIDQWRW RVFDGSITKE NYNQEWWSLR 1100
LKYQGLCPPV PRSQDDFDPG SKFHVPANVP YIRYFVSFII QFQFHEALCR 1150
AAGHTGPLHK CDIYQSKEAG KLLADTMKMG YSKPWPEAMK LITGQPNMSA 1200
SAMMNYFKPL TEWLVTENRR HGETLGWPEY NWTPNTARSE GPFPESGRVN 1250
FLGMYLEPQQ ARVGQWVLLF LGVSLLVATL GLTHRLFSIR QHGHSLHRPH 1300
RGPQFGSEVE LRHS 1314
Length:1,314
Mass (Da):151,595
Last modified:June 7, 2005 - v1
Checksum:i74E29238E87F157E
GO
Isoform Testis-specific (identifier: Q50JE5-2)

Also known as: ACE-T

Sequence is not available
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB212958 mRNA. Translation: BAD98304.1.
RefSeqiNP_001268510.1. NM_001281581.1. [Q50JE5-1]

Genome annotation databases

GeneIDi101824864.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB212958 mRNA. Translation: BAD98304.1 .
RefSeqi NP_001268510.1. NM_001281581.1. [Q50JE5-1 ]

3D structure databases

ProteinModelPortali Q50JE5.
SMRi Q50JE5. Positions 36-647, 651-1234.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M02.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101824864.

Phylogenomic databases

HOVERGENi HBG000264.

Enzyme and pathway databases

BRENDAi 3.4.15.1. 3239.

Family and domain databases

InterProi IPR001548. Peptidase_M2.
[Graphical view ]
PANTHERi PTHR10514. PTHR10514. 1 hit.
Pfami PF01401. Peptidase_M2. 2 hits.
[Graphical view ]
PRINTSi PR00791. PEPDIPTASEA.
PROSITEi PS00142. ZINC_PROTEASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning of hamster angiotensin-converting enzyme and mRNA expression."
    Uchide T., Fujimori Y., Fukushima U., Uechi M., Sasaki T., Temma K.
    DNA Seq. 17:319-325(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Entry informationi

Entry nameiACE_MESAU
AccessioniPrimary (citable) accession number: Q50JE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 7, 2005
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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