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Protein

BTB/POZ domain-containing protein KCTD8

Gene

Kctd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization.1 Publication

GO - Biological processi

  • protein homooligomerization Source: InterPro
  • regulation of G-protein coupled receptor protein signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ domain-containing protein KCTD8
Gene namesi
Name:Kctd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2443804. Kctd8.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • postsynaptic membrane Source: UniProtKB-SubCell
  • presynaptic membrane Source: UniProtKB-SubCell
  • receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476BTB/POZ domain-containing protein KCTD8PRO_0000251482Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphoserineCombined sources
Modified residuei413 – 4131PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ50H33.
PaxDbiQ50H33.
PRIDEiQ50H33.

PTM databases

iPTMnetiQ50H33.
PhosphoSiteiQ50H33.

Expressioni

Gene expression databases

BgeeiQ50H33.
GenevisibleiQ50H33. MM.

Interactioni

Subunit structurei

Interacts as a tetramer with GABRB1 and GABRB2.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055326.

Structurei

3D structure databases

ProteinModelPortaliQ50H33.
SMRiQ50H33. Positions 44-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 12279BTBAdd
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.Curated

Phylogenomic databases

eggNOGiKOG2723. Eukaryota.
ENOG410Z155. LUCA.
GeneTreeiENSGT00760000119013.
HOGENOMiHOG000041197.
HOVERGENiHBG052218.
InParanoidiQ50H33.
OMAiDHEERKH.
OrthoDBiEOG7K3TM1.
PhylomeDBiQ50H33.
TreeFamiTF315332.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q50H33-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALKDTGSGG STILPISEMV SASSSPGAPL AAAPGPCAPS PFPEVVELNV
60 70 80 90 100
GGQVYVTKHS TLLSVPDSTL ASMFSPSSPR GGARRRGDLP RDSRARFFID
110 120 130 140 150
RDGFLFRYVL DYLRDKQLAL PEHFPEKERL LREAEFFQLT DLVKLLSPKV
160 170 180 190 200
TKQNSLNDEC CQSDLEDNVS QGSSDALLLR GAAAGAPSGS GAHGVSGVVG
210 220 230 240 250
GGSAPDKRSG FLTLGYRGSY TTVRDNQADA KFRRVARIMV CGRIALAKEV
260 270 280 290 300
FGDTLNESRD PDRQPEKYTS RFYLKFTYLE QAFDRLSEAG FHMVACNSSG
310 320 330 340 350
TAAFVNQYRD DKIWSSYTEY IFFRPPQKIV SPKQEHEDRK RDKVTDKGSE
360 370 380 390 400
SGTSCNELST SSCDSHSEAS TPQDNPANTQ QAAAHQPNTL TLDRPSRKAP
410 420 430 440 450
VQWMPPPDKR RNSELFQSLI SKSRETNLSK KKVCEKLSVE EEMKKCIQDF
460 470
KKIHIPDCFP ERKRQWQSEL LQKYGL
Length:476
Mass (Da):52,768
Last modified:June 7, 2005 - v1
Checksum:i26EC9C5A673A53DF
GO
Isoform 2 (identifier: Q50H33-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     325-346: PPQKIVSPKQEHEDRKRDKVTD → KETAQRNCFSHFIHHMRVDGKA
     347-476: Missing.

Note: No experimental confirmation available.
Show »
Length:346
Mass (Da):37,981
Checksum:iC6F5A0693E660312
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 451V → I in BAC31296 (PubMed:16141072).Curated
Sequence conflicti82 – 832GA → RAP in BAC31296 (PubMed:16141072).Curated
Sequence conflicti149 – 1491K → E in BAC38532 (PubMed:16141072).Curated
Sequence conflicti159 – 1591E → K in BAC30262 (PubMed:16141072).Curated
Sequence conflicti206 – 2061D → Y in BAC32368 (PubMed:16141072).Curated
Sequence conflicti359 – 3591S → Y in BAC32368 (PubMed:16141072).Curated
Sequence conflicti412 – 4121N → K in BAC31527 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei325 – 34622PPQKI…DKVTD → KETAQRNCFSHFIHHMRVDG KA in isoform 2. 1 PublicationVSP_020761Add
BLAST
Alternative sequencei347 – 476130Missing in isoform 2. 1 PublicationVSP_020762Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY615967 mRNA. Translation: AAU25822.1.
AK039167 mRNA. Translation: BAC30262.2.
AK042569 mRNA. Translation: BAC31296.1.
AK043351 mRNA. Translation: BAC31527.1.
AK045439 mRNA. Translation: BAC32368.1.
AK082563 mRNA. Translation: BAC38532.1.
BC116940 mRNA. Translation: AAI16941.1.
BC116942 mRNA. Translation: AAI16943.1.
CCDSiCCDS19320.1. [Q50H33-1]
RefSeqiNP_780728.3. NM_175519.5. [Q50H33-1]
UniGeneiMm.234821.

Genome annotation databases

EnsembliENSMUST00000054095; ENSMUSP00000055326; ENSMUSG00000037653. [Q50H33-1]
ENSMUST00000087231; ENSMUSP00000084484; ENSMUSG00000037653. [Q50H33-2]
GeneIDi243043.
KEGGimmu:243043.
UCSCiuc008xqj.1. mouse. [Q50H33-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY615967 mRNA. Translation: AAU25822.1.
AK039167 mRNA. Translation: BAC30262.2.
AK042569 mRNA. Translation: BAC31296.1.
AK043351 mRNA. Translation: BAC31527.1.
AK045439 mRNA. Translation: BAC32368.1.
AK082563 mRNA. Translation: BAC38532.1.
BC116940 mRNA. Translation: AAI16941.1.
BC116942 mRNA. Translation: AAI16943.1.
CCDSiCCDS19320.1. [Q50H33-1]
RefSeqiNP_780728.3. NM_175519.5. [Q50H33-1]
UniGeneiMm.234821.

3D structure databases

ProteinModelPortaliQ50H33.
SMRiQ50H33. Positions 44-146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055326.

PTM databases

iPTMnetiQ50H33.
PhosphoSiteiQ50H33.

Proteomic databases

MaxQBiQ50H33.
PaxDbiQ50H33.
PRIDEiQ50H33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054095; ENSMUSP00000055326; ENSMUSG00000037653. [Q50H33-1]
ENSMUST00000087231; ENSMUSP00000084484; ENSMUSG00000037653. [Q50H33-2]
GeneIDi243043.
KEGGimmu:243043.
UCSCiuc008xqj.1. mouse. [Q50H33-1]

Organism-specific databases

CTDi386617.
MGIiMGI:2443804. Kctd8.

Phylogenomic databases

eggNOGiKOG2723. Eukaryota.
ENOG410Z155. LUCA.
GeneTreeiENSGT00760000119013.
HOGENOMiHOG000041197.
HOVERGENiHBG052218.
InParanoidiQ50H33.
OMAiDHEERKH.
OrthoDBiEOG7K3TM1.
PhylomeDBiQ50H33.
TreeFamiTF315332.

Miscellaneous databases

NextBioi385637.
PROiQ50H33.
SOURCEiSearch...

Gene expression databases

BgeeiQ50H33.
GenevisibleiQ50H33. MM.

Family and domain databases

InterProiIPR000210. BTB/POZ_dom.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
[Graphical view]
PfamiPF02214. BTB_2. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conserved paralogous segments on mouse chromosome 5 and 18."
    Odeh H.M., Mitchem K.L., Kohrman D.C.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Corpora quadrigemina and Hypothalamus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78 AND SER-413, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney and Lung.
  5. Cited for: FUNCTION, INTERACTION WITH GABRB1 AND GABRB2, TETRAMERIZATION.

Entry informationi

Entry nameiKCTD8_MOUSE
AccessioniPrimary (citable) accession number: Q50H33
Secondary accession number(s): Q8BR74
, Q8C4C2, Q8C906, Q8C9B0, Q8CAA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: June 7, 2005
Last modified: January 20, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.