Reviewed, UniProtKB/Swiss-Prot Q50EL0 (DMAW_ASPFU)

Names and origin

Protein names

Recommended name:
    Tryptophan dimethylallyltransferase
    EC=2.5.1.34
Alternative name(s):
    L-tryptophan dimethylallyl transferase
      Short name=DMATS
    All-trans-hexaprenyl-diphosphate synthase

Gene names

Name:	fgaPT2
ORF Names:	AFUA_2G18040

Organism

Aspergillus fumigatus (Sartorya fumigata) [Complete proteome]

Taxonomic identifier

5085 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the first step of ergot alkaloid biosynthesis. Ergot alkaloids, which are produced by endophyte fungi, can enhance plant host fitness, but also cause livestock toxicosis to host plants. Ref.1
Catalytic activity	Dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan.
Pathway	Alkaloid biosynthesis; ergot alkaloid biosynthesis.
Subunit structure	Homodimer. Ref.1
Sequence similarities	Belongs to the tryptophan dimethylallyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=4 µM for dimethylallyl diphosphate KM=8 µM for L-tryptophan Vmax=0.198 µmol/min/mg enzyme
Sequence caution	The sequence EAL94103.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tryptophan dimethylallyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Tryptophan dimethylallyltransferase		PRO_0000228687
Natural variations
	Natural variant	443	1	S → A in strain: B5233 / ATCC 13073.

Sequences

	Sequence		Length	Mass (Da)
	Q50EL0-1 [UniParc]. Last modified March 21, 2006. Version 2. Checksum: ED7AAB193E9A46C8	FASTA	459	52,463
	10 20 30 40 50 60 MKAANASSAE AYRVLSRAFR FDNEDQKLWW HSTAPMFAKM LETANYTTPC QYQYLITYKE 70 80 90 100 110 120 CVIPSLGCYP TNSAPRWLSI LTRYGTPFEL SLNCSNSIVR YTFEPINQHT GTDKDPFNTH 130 140 150 160 170 180 AIWESLQHLL PLEKSIDLEW FRHFKHDLTL NSEESAFLAH NDRLVGGTIR TQNKLALDLK 190 200 210 220 230 240 DGRFALKTYI YPALKAVVTG KTIHELVFGS VRRLAVREPR ILPPLNMLEE YIRSRGSKST 250 260 270 280 290 300 ASPRLVSCDL TSPAKSRIKI YLLEQMVSLE AMEDLWTLGG RRRDASTLEG LSLVRELWDL 310 320 330 340 350 360 IQLSPGLKSY PAPYLPLGVI PDERLPLMAN FTLHQNDPVP EPQVYFTTFG MNDMAVADAL 370 380 390 400 410 420 TTFFERRGWS EMARTYETTL KSYYPHADHD KLNYLHAYIS FSYRDRTPYL SVYLQSFETG 430 440 450 DWAVANLSES KVKCQDAACQ PTSLPPDLSK TGVYYSGLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overproduction, purification and characterization of FgaPT2, a dimethylallyltryptophan synthase from Aspergillus fumigatus." Unsoeld I.A., Li S.-M. Microbiology 151:1499-1505(2005) [PubMed: 15870460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: B5233 / ATCC 13073.
[2]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY775787 mRNA. Translation: AAX08549.1.
AAHF01000001 Genomic DNA. Translation: EAL94103.2. Sequence problems.

RefSeq

XP_756141.2.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I4X	X-ray	2.10	A/B	1-459	[»]
3I4Z	X-ray	1.76	A/B	1-459	[»]

ModBase

Search...

Genome annotation databases

GeneID

3512716.

GenomeReviews

Gene locus fgaPT2 in contig CM000170_GR.

KEGG

afm:AFUA_2G18040.

Enzyme and pathway databases

BRENDA

2.5.1.34. 18841.

Family and domain databases

InterPro

IPR017795. Aromatic_prenylTrfase_DMATS.
IPR012148. Trp_dimethylallyltransferase.
IPR017796. Trp_dimethylallylTrfase_sub.
[Graphical view]

PIRSF

PIRSF000509. Trp_DMAT. 1 hit.

TIGRFAMs

TIGR03429. arom_pren_DMATS. 1 hit.
TIGR03430. trp_dimet_allyl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

DMAW_ASPFU

Accession

Primary (citable) accession number: Q50EL0
Secondary accession number(s): Q4WZ62

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	March 21, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)