Reviewed, UniProtKB/Swiss-Prot Q50EK1 (C16B1_PICSI)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 716B1
    EC=1.14.-.-
Alternative name(s):
    Cytochrome P450 CYPA1

Gene names

Name:

CYP716B1

Organism

Picea sitchensis (Sitka spruce)

Taxonomic identifier

3332 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Pinaceae › Picea

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Cofactor	Heme group By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Cytochrome P450 716B1		PRO_0000352517
Regions
	Transmembrane	20 – 40	21	Potential
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50EK1-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 2381E5BFFF06120D	FASTA	493	56,273
	10 20 30 40 50 60 MVWKEAVSVL QKAQELKEPP LMFTVFLASF IGLAFFFYLI SNHRTKAWRG IPPGTFGWPL 70 80 90 100 110 120 IGETLEFLGC QRKGNPRDFF DSRTQKYGNV FTTSLVGHPT VVFCSPEGNR FLFSNENKLV 130 140 150 160 170 180 VNSWPSSVGN LFRSSLITTV GDDAKRLRRI LMTFLRPEAL REFVGRVDSM TKRHLAEHWI 190 200 210 220 230 240 GKDEVMALPL LKRYTFSLAC DLFASINTKD DLDRLWLHFM VFVKGVMQIP IDLPGTRYNK 250 260 270 280 290 300 TKHAANAIRQ QLGSIINERK IALEAGNASP EQDLLSFLLS NVDEQGESLT DNEIQDNILL 310 320 330 340 350 360 LLYAGHDTSS STLTVLLKFL AENPHCYEEV LREQLNIAGS KEEGQLLEWE DLQRMKYSWR 370 380 390 400 410 420 VAQEALRLFP AVQGSFRKAI KEFIYDGFTI PKGWKLHWTV NSTHQKSEYF SNPEKFDPSR 430 440 450 460 470 480 FEGEGPPPYT FVPFGGGPRM CPGNEFARME ILIFLHNIVK NFNWNLVNPL EKVIVDPMPA 490 PVNGLPIKLV PHD

References

[1]

"Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase." Ro D.-K., Arimura G., Lau S.Y.W., Piers E., Bohlmann J. Proc. Natl. Acad. Sci. U.S.A. 102:8060-8065(2005) [PubMed: 15911762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY779542 mRNA. Translation: AAX07436.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.1. 297.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C16B1_PICSI

Accession

Primary (citable) accession number: Q50EK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)