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Q50976 (T2F7_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NgoFVII

Short name=R.NgoFVII
EC=3.1.21.4
Alternative name(s):
Endonuclease NgoFVII
R.NgoVII
Type II restriction enzyme NgoFVII
Gene names
Name:ngoFVIIR
Synonyms:dcrG
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GCSGC and cleaves after G-4.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Type-2 restriction enzyme NgoFVII
PRO_0000077346

Sequences

Sequence LengthMass (Da)Tools
Q50976 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1908B94B4DEDFD25

FASTA32637,528
        10         20         30         40         50         60 
MNTVFSNIAN AKITEKSLNA VWMDLFKSAD EVLMATGYVS NDAVVELHKI LELNDHIQKI 

        70         80         90        100        110        120 
DLLVGMHYLE GFSHLQYDSL CKLNDFLRHE KRGAVYVSPF VKFHGKMYSF KNYQKINGLI 

       130        140        150        160        170        180 
GSANLTCFWD STERTYETML HLNGKPAQIL QADIQSTIHK LGKNIQEVER PSKFIEHNSH 

       190        200        210        220        230        240 
LENCLGVQKI APEQIRQLFA QTSEYHFSIP AKTEEKSNLN VFFGEGRRDK RGFVKPRPWY 

       250        260        270        280        290        300 
EVELIVSKDI TSQEGYPVLK SFTVITDDGW QFQCKTSGDY SKTSTQKMNL KTLGKWIKGR 

       310        320 
LESHGCLQNN EKITHETLRE YGNESF 

« Hide

References

[1]"Restriction and modification systems of Neisseria gonorrhoeae."
Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.
Gene 157:19-22(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1291C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43736 Genomic DNA. Translation: AAA86271.1.
PIRT10166.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3137. NgoFVII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019065. Res_endonuc_III_NgoFVII.
[Graphical view]
PfamPF09565. RE_NgoFVII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2F7_NEIGO
AccessionPrimary (citable) accession number: Q50976
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries