Reviewed, UniProtKB/Swiss-Prot Q50976 (T2F7_NEIGO)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme NgoFVII
      Short name=R.NgoFVII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme NgoFVII
    Endonuclease NgoFVII
    R.NgoVII

Gene names

Name:	ngoFVIIR
Synonyms:	dcrG

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GCSGC and cleaves after G-4.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Type-2 restriction enzyme NgoFVII		PRO_0000077346

Sequences

	Sequence		Length	Mass (Da)
	Q50976-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1908B94B4DEDFD25	FASTA	326	37,528
	10 20 30 40 50 60 MNTVFSNIAN AKITEKSLNA VWMDLFKSAD EVLMATGYVS NDAVVELHKI LELNDHIQKI 70 80 90 100 110 120 DLLVGMHYLE GFSHLQYDSL CKLNDFLRHE KRGAVYVSPF VKFHGKMYSF KNYQKINGLI 130 140 150 160 170 180 GSANLTCFWD STERTYETML HLNGKPAQIL QADIQSTIHK LGKNIQEVER PSKFIEHNSH 190 200 210 220 230 240 LENCLGVQKI APEQIRQLFA QTSEYHFSIP AKTEEKSNLN VFFGEGRRDK RGFVKPRPWY 250 260 270 280 290 300 EVELIVSKDI TSQEGYPVLK SFTVITDDGW QFQCKTSGDY SKTSTQKMNL KTLGKWIKGR 310 320 LESHGCLQNN EKITHETLRE YGNESF

References

[1]	"Restriction and modification systems of Neisseria gonorrhoeae." Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A. Gene 157:19-22(1995) [PubMed: 7607490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1291C.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U43736 Genomic DNA. Translation: AAA86271.1.
PIR	T10166.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3137. NgoFVII.
Enzyme and pathway databases
BRENDA	3.1.21.4. 588.
Family and domain databases
InterPro	IPR019065. Restrict_endonuc_II_NgoFVII. [Graphical view]
Pfam	PF09565. RE_NgoFVII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2F7_NEIGO

Accession

Primary (citable) accession number: Q50976

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)