Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q50975 (T2B5_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NgoBV

Short name=R.NgoBV
EC=3.1.21.4
Alternative name(s):
Endonuclease NgoBV
R.NgoV
Type II restriction enzyme NgoBV
Gene names
Name:ngoBVR
Synonyms:dcrE
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length175 AA.
Sequence statusFragment.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGNNCC and cleaves after N-3.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›175›175Type-2 restriction enzyme NgoBV
PRO_0000077345

Experimental info

Non-terminal residue1751

Sequences

Sequence LengthMass (Da)Tools
Q50975 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 786E8EBCCDF08235

FASTA17520,257
        10         20         30         40         50         60 
MIKLTAQQIF DKLLDEEKIL SANGQIRFFL GDVDIIVKQK DVVGNIIQEW LGGWLRKREI 

        70         80         90        100        110        120 
EFDVSTNTQM PPDFFLNKKD RSRELLEVKA FNRNACPGFD IADFKMYSDE SFISPISGCR 

       130        140        150        160        170 
LFNIGYDMDD NGNVTIKDLW LKKVWQITRS MDGWAINFKS KKAWCIKSAR VFGTA 

« Hide

References

[1]"Restriction and modification systems of Neisseria gonorrhoeae."
Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A.
Gene 157:19-22(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: WR302.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43735 Genomic DNA. Translation: AAA86269.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3134. NgoBV.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019064. Restrct_endonuc_II_NlaIV.
[Graphical view]
PfamPF09564. RE_NgoBV. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2B5_NEIGO
AccessionPrimary (citable) accession number: Q50975
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries