Reviewed, UniProtKB/Swiss-Prot Q50975 (T2B5_NEIGO)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme NgoBV
      Short name=R.NgoBV
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme NgoBV
    Endonuclease NgoBV
    R.NgoV

Gene names

Name:	ngoBVR
Synonyms:	dcrE

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	175 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGNNCC and cleaves after N-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›175	›175	Type-2 restriction enzyme NgoBV		PRO_0000077345
Experimental info
	Non-terminal residue	175	1

Sequences

	Sequence		Length	Mass (Da)
	Q50975-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 786E8EBCCDF08235	FASTA	175	20,257
	10 20 30 40 50 60 MIKLTAQQIF DKLLDEEKIL SANGQIRFFL GDVDIIVKQK DVVGNIIQEW LGGWLRKREI 70 80 90 100 110 120 EFDVSTNTQM PPDFFLNKKD RSRELLEVKA FNRNACPGFD IADFKMYSDE SFISPISGCR 130 140 150 160 170 LFNIGYDMDD NGNVTIKDLW LKKVWQITRS MDGWAINFKS KKAWCIKSAR VFGTA

References

[1]	"Restriction and modification systems of Neisseria gonorrhoeae." Stein D.C., Gunn J.S., Radlinska M., Piekarowicz A. Gene 157:19-22(1995) [PubMed: 7607490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WR302.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U43735 Genomic DNA. Translation: AAA86269.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3134. NgoBV.
Enzyme and pathway databases
BRENDA	3.1.21.4. 588.
Family and domain databases
InterPro	IPR019064. Restrict_endonuc_II_NgoBV. [Graphical view]
Pfam	PF09564. RE_NgoBV. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2B5_NEIGO

Accession

Primary (citable) accession number: Q50975

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)