Q50973 (T2B1_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme NgoBI
Short name=R.NgoBI
EC=3.1.21.4

Alternative name(s):
Endonuclease NgoBI
R.NgoI
Type II restriction enzyme NgoBI

Gene names

Name:

ngoBIR

Organism

Neisseria gonorrhoeae

Taxonomic identifier

485 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence RGCGCY and cleaves after C-5.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Type-2 restriction enzyme NgoBI		PRO_0000077344

Sequences

	Sequence		Length	Mass (Da)
	Q50973 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: EF3EC3540B55E2EF	FASTA	350	40,638
	10 20 30 40 50 60 MTLEEQQAKE ALDGIIKKSR VHLYKPIQIA EILYHDRCIK QLDFLNLDTY RNQSKRWRDE 70 80 90 100 110 120 ICRRFLGRIS TSSAKFQDNL FEKNAIPPEK LAVLGTLNRQ SDGGVESYIY KQFFNRFSQM 130 140 150 160 170 180 SERLAYVGNT DRYSFQLSEF LNLFWLEPGL KRSIDKIYEI VVYALFDALV SELGITVSID 190 200 210 220 230 240 FPKENLFLWE EYQDFAEKII TMPKNEHLKL PAKIHRVGVT NAADRGLDMW SNFGLAIQVK 250 260 270 280 290 300 HLSLDEELAE DIVSSISADR IVIVCKKAEQ SVIVSLLTQI GWKSRIQNIV TEDDLISWYE 310 320 330 340 350 KALRGQYPIA EALLENIKTE IMREFPAVNE ANEFLDFAQN RGYDITVTHF

References

[1]	"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems." Stein D.C., Gunn J.S., Piekarowicz A. Biol. Chem. 379:575-578(1998) [PubMed: 9628358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WR302.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U42459 Genomic DNA. Translation: AAB03207.2.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3133. NgoBI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019058. Restrct_endonuc_II_HaeII. [Graphical view]
Pfam	PF09554. RE_HaeII. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2B1_NEIGO

Accession

Primary (citable) accession number: Q50973

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	May 31, 2011
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program