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Protein

Type-2 restriction enzyme NgoBI

Gene

ngoBIR

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence RGCGCY and cleaves after C-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi3133. NgoBI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme NgoBI (EC:3.1.21.4)
Short name:
R.NgoBI
Alternative name(s):
Endonuclease NgoBI
R.NgoI
Type II restriction enzyme NgoBI
Gene namesi
Name:ngoBIR
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350Type-2 restriction enzyme NgoBIPRO_0000077344Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019058. Restrct_endonuc_II_HaeII.
[Graphical view]
PfamiPF09554. RE_HaeII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50973-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLEEQQAKE ALDGIIKKSR VHLYKPIQIA EILYHDRCIK QLDFLNLDTY
60 70 80 90 100
RNQSKRWRDE ICRRFLGRIS TSSAKFQDNL FEKNAIPPEK LAVLGTLNRQ
110 120 130 140 150
SDGGVESYIY KQFFNRFSQM SERLAYVGNT DRYSFQLSEF LNLFWLEPGL
160 170 180 190 200
KRSIDKIYEI VVYALFDALV SELGITVSID FPKENLFLWE EYQDFAEKII
210 220 230 240 250
TMPKNEHLKL PAKIHRVGVT NAADRGLDMW SNFGLAIQVK HLSLDEELAE
260 270 280 290 300
DIVSSISADR IVIVCKKAEQ SVIVSLLTQI GWKSRIQNIV TEDDLISWYE
310 320 330 340 350
KALRGQYPIA EALLENIKTE IMREFPAVNE ANEFLDFAQN RGYDITVTHF
Length:350
Mass (Da):40,638
Last modified:April 30, 2000 - v2
Checksum:iEF3EC3540B55E2EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42459 Genomic DNA. Translation: AAB03207.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42459 Genomic DNA. Translation: AAB03207.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3133. NgoBI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019058. Restrct_endonuc_II_HaeII.
[Graphical view]
PfamiPF09554. RE_HaeII. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
    Stein D.C., Gunn J.S., Piekarowicz A.
    Biol. Chem. 379:575-578(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WR302.

Entry informationi

Entry nameiT2B1_NEIGO
AccessioniPrimary (citable) accession number: Q50973
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: April 30, 2000
Last modified: April 15, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.