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Q50973

- T2B1_NEIGO

UniProt

Q50973 - T2B1_NEIGO

Protein

Type-2 restriction enzyme NgoBI

Gene

ngoBIR

Organism
Neisseria gonorrhoeae
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 44 (16 Apr 2014)
      Sequence version 2 (01 May 2000)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence RGCGCY and cleaves after C-5.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi3133. NgoBI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme NgoBI (EC:3.1.21.4)
    Short name:
    R.NgoBI
    Alternative name(s):
    Endonuclease NgoBI
    R.NgoI
    Type II restriction enzyme NgoBI
    Gene namesi
    Name:ngoBIR
    OrganismiNeisseria gonorrhoeae
    Taxonomic identifieri485 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350Type-2 restriction enzyme NgoBIPRO_0000077344Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019058. Restrct_endonuc_II_HaeII.
    [Graphical view]
    PfamiPF09554. RE_HaeII. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q50973-1 [UniParc]FASTAAdd to Basket

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    MTLEEQQAKE ALDGIIKKSR VHLYKPIQIA EILYHDRCIK QLDFLNLDTY    50
    RNQSKRWRDE ICRRFLGRIS TSSAKFQDNL FEKNAIPPEK LAVLGTLNRQ 100
    SDGGVESYIY KQFFNRFSQM SERLAYVGNT DRYSFQLSEF LNLFWLEPGL 150
    KRSIDKIYEI VVYALFDALV SELGITVSID FPKENLFLWE EYQDFAEKII 200
    TMPKNEHLKL PAKIHRVGVT NAADRGLDMW SNFGLAIQVK HLSLDEELAE 250
    DIVSSISADR IVIVCKKAEQ SVIVSLLTQI GWKSRIQNIV TEDDLISWYE 300
    KALRGQYPIA EALLENIKTE IMREFPAVNE ANEFLDFAQN RGYDITVTHF 350
    Length:350
    Mass (Da):40,638
    Last modified:May 1, 2000 - v2
    Checksum:iEF3EC3540B55E2EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U42459 Genomic DNA. Translation: AAB03207.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U42459 Genomic DNA. Translation: AAB03207.2 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3133. NgoBI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019058. Restrct_endonuc_II_HaeII.
    [Graphical view ]
    Pfami PF09554. RE_HaeII. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
      Stein D.C., Gunn J.S., Piekarowicz A.
      Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: WR302.

    Entry informationi

    Entry nameiT2B1_NEIGO
    AccessioniPrimary (citable) accession number: Q50973
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 2000
    Last modified: April 16, 2014
    This is version 44 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3