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Q50973 (T2B1_NEIGO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme NgoBI

Short name=R.NgoBI
EC=3.1.21.4
Alternative name(s):
Endonuclease NgoBI
R.NgoI
Type II restriction enzyme NgoBI
Gene names
Name:ngoBIR
OrganismNeisseria gonorrhoeae
Taxonomic identifier485 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence RGCGCY and cleaves after C-5.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350Type-2 restriction enzyme NgoBI
PRO_0000077344

Sequences

Sequence LengthMass (Da)Tools
Q50973 [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: EF3EC3540B55E2EF

FASTA35040,638
        10         20         30         40         50         60 
MTLEEQQAKE ALDGIIKKSR VHLYKPIQIA EILYHDRCIK QLDFLNLDTY RNQSKRWRDE 

        70         80         90        100        110        120 
ICRRFLGRIS TSSAKFQDNL FEKNAIPPEK LAVLGTLNRQ SDGGVESYIY KQFFNRFSQM 

       130        140        150        160        170        180 
SERLAYVGNT DRYSFQLSEF LNLFWLEPGL KRSIDKIYEI VVYALFDALV SELGITVSID 

       190        200        210        220        230        240 
FPKENLFLWE EYQDFAEKII TMPKNEHLKL PAKIHRVGVT NAADRGLDMW SNFGLAIQVK 

       250        260        270        280        290        300 
HLSLDEELAE DIVSSISADR IVIVCKKAEQ SVIVSLLTQI GWKSRIQNIV TEDDLISWYE 

       310        320        330        340        350 
KALRGQYPIA EALLENIKTE IMREFPAVNE ANEFLDFAQN RGYDITVTHF 

« Hide

References

[1]"Sequence similarities between the genes encoding the S.NgoI and HaeII restriction/modification systems."
Stein D.C., Gunn J.S., Piekarowicz A.
Biol. Chem. 379:575-578(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: WR302.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U42459 Genomic DNA. Translation: AAB03207.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3133. NgoBI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019058. Restrct_endonuc_II_HaeII.
[Graphical view]
PfamPF09554. RE_HaeII. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2B1_NEIGO
AccessionPrimary (citable) accession number: Q50973
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries