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Q50788 (NIFD_METTM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitrogenase molybdenum-iron protein alpha chain

EC=1.18.6.1
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene names
Name:nifD
Ordered Locus Names:MTBMA_c01490
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activity

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactor

Binds 1 8Fe-7S cluster per heterodimer By similarity.

Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit By similarity.

Subunit structure

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Sequence similarities

Belongs to the NifD/NifK/NifE/NifN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470Nitrogenase molybdenum-iron protein alpha chain
PRO_0000153074

Sites

Metal binding481Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding741Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding1471Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding2651Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl) By similarity
Metal binding4311Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogen By similarity

Experimental info

Sequence conflict3481N → I in CAA61219. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q50788 [UniParc].

Last modified November 30, 2010. Version 3.
Checksum: 9419CE510DB68CD9

FASTA47053,057
        10         20         30         40         50         60 
MPFELFGVDA EIPDRKKHIY VKKQGDPEGD IPACNTTTIP GCMTERGCAF AGAKGVITGA 

        70         80         90        100        110        120 
IKDALHVIHS PVGCTAYGYG TKRYPTSQEM PDGSMFPIEK FNLKYITGTD LSESDVVFGG 

       130        140        150        160        170        180 
MDKLKRCILE AVREFPEANA VYTYATCTTG LIGDDIDAIS REVSEEIGKD VVAINAPGFA 

       190        200        210        220        230        240 
GPTQSKGHQV ANYTLFEDLV GTAEPPRTTE YDVNLIGEYN IDGDLWVLKK YFEEMGINVL 

       250        260        270        280        290        300 
STFTGDCCHD EIKWMHRAKL SLVRCQRSAN YIAKLLEERY GVPYMKVDFF GIEYCRKNLM 

       310        320        330        340        350        360 
AIGEYFGIPE RAERVIEDRM KKIGPEIQYF KDKLRGKRVW VFSGGPKNWH LPRPLEDELG 

       370        380        390        400        410        420 
MEVVAVSTMF EHEDGYEKIK KRVREGTVIV DDPNSLELEE IIEKYRPDII LSGIKEKYLA 

       430        440        450        460        470 
HKLGVPCILI HSYENGPYIG FEGFLNLARD MYAAIYNPVW DLLEFEEDVN 

« Hide

References

« Hide 'large scale' references
[1]Hochheimer A.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[3]"The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide."
Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R.
Eur. J. Biochem. 234:910-920(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-220.
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X87971 Genomic DNA. Translation: CAA61219.1.
CP001710 Genomic DNA. Translation: ADL57758.1.
PIRS63551.
RefSeqYP_003849071.1. NC_014408.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL57758; ADL57758; MTBMA_c01490.
GeneID9703854.
KEGGmmg:MTBMA_c01490.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000005528.
KOK02586.
OMAQLKYTYA.

Enzyme and pathway databases

BioCycMMAR79929:GH5J-149-MONOMER.

Family and domain databases

InterProIPR000510. Nase/OxRdtase_comp1.
IPR005974. Nase_asu.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
[Graphical view]
PfamPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsTIGR01284. alt_nitrog_alph. 1 hit.
TIGR01862. N2-ase-Ialpha. 1 hit.
PROSITEPS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIFD_METTM
AccessionPrimary (citable) accession number: Q50788
Secondary accession number(s): D9PU60, P97185
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 30, 2010
Last modified: May 14, 2014
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families