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Protein

Nitrogenase molybdenum-iron protein alpha chain

Gene

nifD

Organism
Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi74Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi147Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi265Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl)By similarity1
Metal bindingi431Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
LigandATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR79929:G1GML-146-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifD
Ordered Locus Names:MTBMA_c01490
OrganismiMethanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
Proteomesi
  • UP000000345 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001530741 – 470Nitrogenase molybdenum-iron protein alpha chainAdd BLAST470

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Protein-protein interaction databases

STRINGi79929.MTBMA_c01490

Structurei

3D structure databases

SMRiQ50788
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Phylogenomic databases

eggNOGiarCOG00598 Archaea
COG2710 LUCA
HOGENOMiHOG000005528
KOiK02586
OMAiYCFSTDM
OrthoDBiPOG093Z01NS

Family and domain databases

InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR005974 Nase_asu
IPR010143 Nase_comp1_asu
IPR000318 Nase_comp1_CS
PANTHERiPTHR43457 PTHR43457, 1 hit
PfamiView protein in Pfam
PF00148 Oxidored_nitro, 1 hit
TIGRFAMsiTIGR01284 alt_nitrog_alph, 1 hit
TIGR01862 N2-ase-Ialpha, 1 hit
PROSITEiView protein in PROSITE
PS00090 NITROGENASE_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q50788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFELFGVDA EIPDRKKHIY VKKQGDPEGD IPACNTTTIP GCMTERGCAF
60 70 80 90 100
AGAKGVITGA IKDALHVIHS PVGCTAYGYG TKRYPTSQEM PDGSMFPIEK
110 120 130 140 150
FNLKYITGTD LSESDVVFGG MDKLKRCILE AVREFPEANA VYTYATCTTG
160 170 180 190 200
LIGDDIDAIS REVSEEIGKD VVAINAPGFA GPTQSKGHQV ANYTLFEDLV
210 220 230 240 250
GTAEPPRTTE YDVNLIGEYN IDGDLWVLKK YFEEMGINVL STFTGDCCHD
260 270 280 290 300
EIKWMHRAKL SLVRCQRSAN YIAKLLEERY GVPYMKVDFF GIEYCRKNLM
310 320 330 340 350
AIGEYFGIPE RAERVIEDRM KKIGPEIQYF KDKLRGKRVW VFSGGPKNWH
360 370 380 390 400
LPRPLEDELG MEVVAVSTMF EHEDGYEKIK KRVREGTVIV DDPNSLELEE
410 420 430 440 450
IIEKYRPDII LSGIKEKYLA HKLGVPCILI HSYENGPYIG FEGFLNLARD
460 470
MYAAIYNPVW DLLEFEEDVN
Length:470
Mass (Da):53,057
Last modified:November 30, 2010 - v3
Checksum:i9419CE510DB68CD9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348N → I in CAA61219 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87971 Genomic DNA Translation: CAA61219.1
CP001710 Genomic DNA Translation: ADL57758.1
PIRiS63551
RefSeqiWP_013294986.1, NC_014408.1

Genome annotation databases

EnsemblBacteriaiADL57758; ADL57758; MTBMA_c01490
GeneIDi9703854
KEGGimmg:MTBMA_c01490
PATRICifig|79929.8.peg.145

Similar proteinsi

Entry informationi

Entry nameiNIFD_METTM
AccessioniPrimary (citable) accession number: Q50788
Secondary accession number(s): D9PU60, P97185
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 30, 2010
Last modified: March 28, 2018
This is version 90 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health