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Q50788

- NIFD_METTM

UniProt

Q50788 - NIFD_METTM

Protein

Nitrogenase molybdenum-iron protein alpha chain

Gene

nifD

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 3 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

    Catalytic activityi

    8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

    Cofactori

    Binds 1 8Fe-7S cluster per heterodimer.By similarity
    Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi48 – 481Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
    Metal bindingi74 – 741Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
    Metal bindingi147 – 1471Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
    Metal bindingi265 – 2651Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl)By similarity
    Metal bindingi431 – 4311Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogenBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
    3. iron-sulfur cluster binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. nitrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMAR79929:GH5J-149-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
    Alternative name(s):
    Dinitrogenase
    Nitrogenase component I
    Gene namesi
    Name:nifD
    Ordered Locus Names:MTBMA_c01490
    OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri79929 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000000345: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470Nitrogenase molybdenum-iron protein alpha chainPRO_0000153074Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NifD/NifK/NifE/NifN family.Curated

    Phylogenomic databases

    HOGENOMiHOG000005528.
    KOiK02586.
    OMAiQLKYTYA.

    Family and domain databases

    InterProiIPR000510. Nase/OxRdtase_comp1.
    IPR005974. Nase_asu.
    IPR010143. Nase_comp1_asu.
    IPR000318. Nase_comp1_CS.
    [Graphical view]
    PfamiPF00148. Oxidored_nitro. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01284. alt_nitrog_alph. 1 hit.
    TIGR01862. N2-ase-Ialpha. 1 hit.
    PROSITEiPS00090. NITROGENASE_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q50788-1 [UniParc]FASTAAdd to Basket

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    MPFELFGVDA EIPDRKKHIY VKKQGDPEGD IPACNTTTIP GCMTERGCAF    50
    AGAKGVITGA IKDALHVIHS PVGCTAYGYG TKRYPTSQEM PDGSMFPIEK 100
    FNLKYITGTD LSESDVVFGG MDKLKRCILE AVREFPEANA VYTYATCTTG 150
    LIGDDIDAIS REVSEEIGKD VVAINAPGFA GPTQSKGHQV ANYTLFEDLV 200
    GTAEPPRTTE YDVNLIGEYN IDGDLWVLKK YFEEMGINVL STFTGDCCHD 250
    EIKWMHRAKL SLVRCQRSAN YIAKLLEERY GVPYMKVDFF GIEYCRKNLM 300
    AIGEYFGIPE RAERVIEDRM KKIGPEIQYF KDKLRGKRVW VFSGGPKNWH 350
    LPRPLEDELG MEVVAVSTMF EHEDGYEKIK KRVREGTVIV DDPNSLELEE 400
    IIEKYRPDII LSGIKEKYLA HKLGVPCILI HSYENGPYIG FEGFLNLARD 450
    MYAAIYNPVW DLLEFEEDVN 470
    Length:470
    Mass (Da):53,057
    Last modified:November 30, 2010 - v3
    Checksum:i9419CE510DB68CD9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti348 – 3481N → I in CAA61219. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87971 Genomic DNA. Translation: CAA61219.1.
    CP001710 Genomic DNA. Translation: ADL57758.1.
    PIRiS63551.
    RefSeqiYP_003849071.1. NC_014408.1.

    Genome annotation databases

    EnsemblBacteriaiADL57758; ADL57758; MTBMA_c01490.
    GeneIDi9703854.
    KEGGimmg:MTBMA_c01490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87971 Genomic DNA. Translation: CAA61219.1 .
    CP001710 Genomic DNA. Translation: ADL57758.1 .
    PIRi S63551.
    RefSeqi YP_003849071.1. NC_014408.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL57758 ; ADL57758 ; MTBMA_c01490 .
    GeneIDi 9703854.
    KEGGi mmg:MTBMA_c01490.

    Phylogenomic databases

    HOGENOMi HOG000005528.
    KOi K02586.
    OMAi QLKYTYA.

    Enzyme and pathway databases

    BioCyci MMAR79929:GH5J-149-MONOMER.

    Family and domain databases

    InterProi IPR000510. Nase/OxRdtase_comp1.
    IPR005974. Nase_asu.
    IPR010143. Nase_comp1_asu.
    IPR000318. Nase_comp1_CS.
    [Graphical view ]
    Pfami PF00148. Oxidored_nitro. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01284. alt_nitrog_alph. 1 hit.
    TIGR01862. N2-ase-Ialpha. 1 hit.
    PROSITEi PS00090. NITROGENASE_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Hochheimer A.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
    2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
      Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
      J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
    3. "The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide."
      Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R.
      Eur. J. Biochem. 234:910-920(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-220.
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

    Entry informationi

    Entry nameiNIFD_METTM
    AccessioniPrimary (citable) accession number: Q50788
    Secondary accession number(s): D9PU60, P97185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 76 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3