Q50788 (NIFD_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogenase molybdenum-iron protein alpha chain
EC=1.18.6.1

Alternative name(s):
Dinitrogenase
Nitrogenase component I

Gene names

Name:	nifD
Ordered Locus Names:	MTBMA_c01490

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanothermobacter marburgensis

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
Catalytic activity	8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.
Cofactor	Binds 1 8Fe-7S cluster per heterodimer By similarity. Binds 1 7Fe-Mo-9S-X-homocitryl cluster per subunit. The identity of the X atom is not known, possibly carbon or oxygen By similarity.
Subunit structure	Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).
Sequence similarities	Belongs to the NifD/NifK/NifE/NifN family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Ligand	ATP-binding Iron Iron-sulfur Metal-binding Molybdenum Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbonyl sulfide nitrogenase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Nitrogenase molybdenum-iron protein alpha chain		PRO_0000153074
Sites
	Metal binding	48	1	Iron-sulfur (8Fe-7S); shared with beta chain By similarity
	Metal binding	74	1	Iron-sulfur (8Fe-7S); shared with beta chain By similarity
	Metal binding	147	1	Iron-sulfur (8Fe-7S); shared with beta chain By similarity
	Metal binding	265	1	Molybdenum-iron-sulfur (7Fe-Mo-9S-X-homocitryl) By similarity
	Metal binding	431	1	Molybdenum-iron-sulfur (7Fe-Mo-9S-X-homocitryl); via pros nitrogen By similarity
Experimental info
	Sequence conflict	348	1	N → I in CAA61219. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q50788 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: 9419CE510DB68CD9	FASTA	470	53,057
	10 20 30 40 50 60 MPFELFGVDA EIPDRKKHIY VKKQGDPEGD IPACNTTTIP GCMTERGCAF AGAKGVITGA 70 80 90 100 110 120 IKDALHVIHS PVGCTAYGYG TKRYPTSQEM PDGSMFPIEK FNLKYITGTD LSESDVVFGG 130 140 150 160 170 180 MDKLKRCILE AVREFPEANA VYTYATCTTG LIGDDIDAIS REVSEEIGKD VVAINAPGFA 190 200 210 220 230 240 GPTQSKGHQV ANYTLFEDLV GTAEPPRTTE YDVNLIGEYN IDGDLWVLKK YFEEMGINVL 250 260 270 280 290 300 STFTGDCCHD EIKWMHRAKL SLVRCQRSAN YIAKLLEERY GVPYMKVDFF GIEYCRKNLM 310 320 330 340 350 360 AIGEYFGIPE RAERVIEDRM KKIGPEIQYF KDKLRGKRVW VFSGGPKNWH LPRPLEDELG 370 380 390 400 410 420 MEVVAVSTMF EHEDGYEKIK KRVREGTVIV DDPNSLELEE IIEKYRPDII LSGIKEKYLA 430 440 450 460 470 HKLGVPCILI HSYENGPYIG FEGFLNLARD MYAAIYNPVW DLLEFEEDVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hochheimer A. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[3]	"The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide." Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R. Eur. J. Biochem. 234:910-920(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-220. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87971 Genomic DNA. Translation: CAA61219.1. CP001710 Genomic DNA. Translation: ADL57758.1.
PIR	S63551.
RefSeq	YP_003849071.1. NC_014408.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADL57758; ADL57758; MTBMA_c01490.
GeneID	9703854.
KEGG	mmg:MTBMA_c01490.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000005528.
KO	K02586.
OMA	LTRRNQT.
Enzyme and pathway databases
BioCyc	MMAR79929:GH5J-153-MONOMER.
Family and domain databases
InterPro	IPR000510. Nase/OxRdtase_comp1. IPR005974. Nase_asu. IPR010143. Nase_comp1_asu. IPR000318. Nase_comp1_CS. [Graphical view]
Pfam	PF00148. Oxidored_nitro. 1 hit. [Graphical view]
TIGRFAMs	TIGR01284. alt_nitrog_alph. 1 hit. TIGR01862. N2-ase-Ialpha. 1 hit.
PROSITE	PS00699. NITROGENASE_1_1. False negative. PS00090. NITROGENASE_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIFD_METTM

Accession

Primary (citable) accession number: Q50788
Secondary accession number(s): D9PU60, P97185

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program