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Q50788

- NIFD_METTM

UniProt

Q50788 - NIFD_METTM

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Protein

Nitrogenase molybdenum-iron protein alpha chain

Gene

nifD

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

Binds 1 8Fe-7S cluster per heterodimer.By similarity
Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
Metal bindingi74 – 741Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
Metal bindingi147 – 1471Iron-sulfur (8Fe-7S); shared with beta chainBy similarity
Metal bindingi265 – 2651Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl)By similarity
Metal bindingi431 – 4311Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogenBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
  3. iron-sulfur cluster binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
  5. nitrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-149-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifD
Ordered Locus Names:MTBMA_c01490
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470Nitrogenase molybdenum-iron protein alpha chainPRO_0000153074Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Phylogenomic databases

HOGENOMiHOG000005528.
KOiK02586.
OMAiQLKYTYA.

Family and domain databases

InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005974. Nase_asu.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01284. alt_nitrog_alph. 1 hit.
TIGR01862. N2-ase-Ialpha. 1 hit.
PROSITEiPS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q50788-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPFELFGVDA EIPDRKKHIY VKKQGDPEGD IPACNTTTIP GCMTERGCAF
60 70 80 90 100
AGAKGVITGA IKDALHVIHS PVGCTAYGYG TKRYPTSQEM PDGSMFPIEK
110 120 130 140 150
FNLKYITGTD LSESDVVFGG MDKLKRCILE AVREFPEANA VYTYATCTTG
160 170 180 190 200
LIGDDIDAIS REVSEEIGKD VVAINAPGFA GPTQSKGHQV ANYTLFEDLV
210 220 230 240 250
GTAEPPRTTE YDVNLIGEYN IDGDLWVLKK YFEEMGINVL STFTGDCCHD
260 270 280 290 300
EIKWMHRAKL SLVRCQRSAN YIAKLLEERY GVPYMKVDFF GIEYCRKNLM
310 320 330 340 350
AIGEYFGIPE RAERVIEDRM KKIGPEIQYF KDKLRGKRVW VFSGGPKNWH
360 370 380 390 400
LPRPLEDELG MEVVAVSTMF EHEDGYEKIK KRVREGTVIV DDPNSLELEE
410 420 430 440 450
IIEKYRPDII LSGIKEKYLA HKLGVPCILI HSYENGPYIG FEGFLNLARD
460 470
MYAAIYNPVW DLLEFEEDVN
Length:470
Mass (Da):53,057
Last modified:November 30, 2010 - v3
Checksum:i9419CE510DB68CD9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti348 – 3481N → I in CAA61219. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87971 Genomic DNA. Translation: CAA61219.1.
CP001710 Genomic DNA. Translation: ADL57758.1.
PIRiS63551.
RefSeqiYP_003849071.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL57758; ADL57758; MTBMA_c01490.
GeneIDi9703854.
KEGGimmg:MTBMA_c01490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87971 Genomic DNA. Translation: CAA61219.1 .
CP001710 Genomic DNA. Translation: ADL57758.1 .
PIRi S63551.
RefSeqi YP_003849071.1. NC_014408.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADL57758 ; ADL57758 ; MTBMA_c01490 .
GeneIDi 9703854.
KEGGi mmg:MTBMA_c01490.

Phylogenomic databases

HOGENOMi HOG000005528.
KOi K02586.
OMAi QLKYTYA.

Enzyme and pathway databases

BioCyci MMAR79929:GH5J-149-MONOMER.

Family and domain databases

InterProi IPR000510. Nase/OxRdtase_comp1.
IPR005974. Nase_asu.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
[Graphical view ]
Pfami PF00148. Oxidored_nitro. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01284. alt_nitrog_alph. 1 hit.
TIGR01862. N2-ase-Ialpha. 1 hit.
PROSITEi PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Hochheimer A.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  3. "The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide."
    Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R.
    Eur. J. Biochem. 234:910-920(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-220.
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiNIFD_METTM
AccessioniPrimary (citable) accession number: Q50788
Secondary accession number(s): D9PU60, P97185
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 30, 2010
Last modified: October 1, 2014
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3