Reviewed, UniProtKB/Swiss-Prot Q50783 (MVHA_METTH)

Names and origin

Protein names

Recommended name:
    F420-non-reducing hydrogenase subunit A
    EC=1.12.99.-
Alternative name(s):
    Methyl viologen-reducing hydrogenase subunit alpha
      Short name=MVH subunit A

Gene names

Name:	mvhA
Ordered Locus Names:	MTH_1134

Organism

Methanobacterium thermoautotrophicum [Complete proteome] [HAMAP]

Taxonomic identifier

187420 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of a complex that provides reducing equivalents for heterodisulfide reductase By similarity.
Cofactor	Nickel Potential.
Subunit structure	The F420-non-reducing hydrogenase is composed of three subunits; mvhA, mvhD and mvhG. It forms a complex with the heterodisulfide reductase (hdr) By similarity.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	F420-non-reducing hydrogenase subunit A		PRO_0000199726
Sites
	Metal binding	61	1	Nickel Potential
	Metal binding	64	1	Nickel Potential
	Metal binding	442	1	Nickel Potential
	Metal binding	445	1	Nickel Potential
Experimental info
	Sequence conflict	21	1	H → R in AAB02351. Ref.1
	Sequence conflict	267	1	K → T in AAB02351. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q50783-1 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: 81A50D0D3156CD90	FASTA	472	52,916
	10 20 30 40 50 60 MVKLTMEPVT RIEGHAKITV HLDDAGNVED TRLHVMEFRG FEKFLQGRPI EEAPRIVPRI 70 80 90 100 110 120 CGICDVQHHL AAAKAVDACF GFEPEDVLPA AYKMREIMNW GSYMHSHGLH FYFLAAPDFI 130 140 150 160 170 180 AGKDRKTRNV FQIIKDAPDI ALQAIELRKN ALELVRATGG RPIHPTSSTP GGISTELDDE 190 200 210 220 230 240 TQKDLLKKAQ RNVELAEATL ELAVPIFEEN IDLVNSLGNI ETYHTGLVKD GVWDVYDGIV 250 260 270 280 290 300 RIKDKEGNMF REFKPADYAD TIAEHVKPYS WLKFPYIKDL GYPDGVYRVS PLSRLNVADK 310 320 330 340 350 360 MPDAAPKAQE HFKEFRENFG YAQQTLLYHW ARLIELLACA ECAADALEGD LSGEKFPDSL 370 380 390 400 410 420 ERQAGDGVGI VEAPRGTLTH HYTCDENGLI TKANIVVATI QNNPAMEMGI QKVAQDYIKP 430 440 450 460 470 GVEVDDKIFN LMEMVIRAYD PCLSCATHTI DSQMRLATLE VYDSEGDLVK RI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain delta H encodes a polyferredoxin." Reeve J.N., Beckler G.S., Cram D.S., Hamilton P.T., Brown J.W., Krzycki J.A., Kolodziej A.F., Alex L., Orme-Johnson W.H., Walsh C.T. Proc. Natl. Acad. Sci. U.S.A. 86:3031-3035(1989) [PubMed: 2654933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Delta H.
[2]	"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics." Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R. , Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N. J. Bacteriol. 179:7135-7155(1997) [PubMed: 9371463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Delta H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04540 Genomic DNA. Translation: AAB02351.1. AE000666 Genomic DNA. Translation: AAB85623.1.
PIR	A69018. F30315.
RefSeq	NP_276262.1.
3D structure databases
HSSP	HSSP built from PDB template 1CC1 based on UniProtKB P13065.
ModBase	Search...
Genome annotation databases
GeneID	1471542.
GenomeReviews	Gene locus MTH_1134 in contig AE000666_GR.
KEGG	mth:MTH1134.
NMPDR	fig|187420.1.peg.1117.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q50783.
OMA	Q50783. LSCATHT.
Enzyme and pathway databases
BioCyc	MTHE187420:MTH1134-MON.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MVHA_METTH

Accession

Primary (citable) accession number: Q50783
Secondary accession number(s): O27206

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	June 16, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)