Q50637 (APT_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine phosphoribosyltransferase
Short name=APRT
EC=2.4.2.7

Gene names

Name:	apt
Ordered Locus Names:	Rv2584c, MT2661
ORF Names:	MTCY227.17

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. HAMAP MF_00004
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00004
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1. HAMAP MF_00004
Subunit structure	Homodimer By similarity. HAMAP MF_00004
Subcellular location	Cytoplasm HAMAP MF_00004.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adenine salvage Inferred from electronic annotation. Source: InterPro purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	adenine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Adenine phosphoribosyltransferase HAMAP MF_00004		PRO_0000149420
Experimental info
	Sequence conflict	147	1	A → E in AAK46974. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q50637 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E91900FA424DB1A3	FASTA	223	23,188
	10 20 30 40 50 60 MCHGGTWAGD YVLNVIATGL SLKARGKRRR QRWVDDGRVL ALGESRRSSA ISVADVVASL 70 80 90 100 110 120 TRDVADFPVP GVEFKDLTPL FADRRGLAAV TEALADRASG ADLVAGVDAR GFLVAAAVAT 130 140 150 160 170 180 RLEVGVLAVR KGGKLPRPVL SEEYYRAYGA ATLEILAEGI EVAGRRVVII DDVLATGGTI 190 200 210 220 GATRRLLERG GANVAGAAVV VELAGLSGRA ALAPLPVHSL SRL

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842580 Genomic DNA. Translation: CAB01259.1. AE000516 Genomic DNA. Translation: AAK46974.1.
PIR	G70725.
RefSeq	NP_217100.1. NC_000962.2. NP_337160.1. NC_002755.2.
3D structure databases
ProteinModelPortal	Q50637.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001839; EBMYCP00000001839; EBMYCG00000001837. EBMYCT00000071045; EBMYCP00000069104; EBMYCG00000071040.
GeneID	888579. 925872.
GenomeReviews	Gene locus MT2661 in contig AE000516_GR. Gene locus Rv2584c in contig AL123456_GR.
KEGG	mtc:MT2661. mtu:Rv2584c.
PATRIC	18127596. VBIMycTub22151_2901.
TIGR	MT2661.
Organism-specific databases
TubercuList	Rv2584c.
Phylogenomic databases
GeneTree	EBGT00050000016328.
HOGENOM	HBG703830.
OMA	GMEARGF.
PhylomeDB	Q50637.
ProtClustDB	PRK02304.
Family and domain databases
HAMAP	MF_00004. Aden_phosphoribosyltr. Divergent sequence. [Tree]
InterPro	IPR005764. Ade_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
KO	K00759.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APT_MYCTU

Accession

Primary (citable) accession number: Q50637

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program