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Q505J9 (ATAD1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATPase family AAA domain-containing protein 1

EC=3.6.1.3
Alternative name(s):
Thorase
Gene names
Name:Atad1
Synonyms:Npg6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent. Ref.3

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with GRIA2 and GRIP1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts not only its binding to GRIA2 and GRIP1, but also interaction between GRIP1 and GRIA2, leading to AMPAR complex disassembly. Ref.3

Subcellular location

Peroxisome By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity.

Sequence similarities

Belongs to the AAA ATPase family.

Biophysicochemical properties

Kinetic parameters:

KM=43.4 mM for ATP Ref.3

Vmax=11.0 nM/min/mg enzyme

Sequence caution

The sequence ABX10437.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Gria2P194913EBI-4280289,EBI-77718

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361ATPase family AAA domain-containing protein 1
PRO_0000084793

Regions

Nucleotide binding133 – 1408ATP Potential

Amino acid modifications

Cross-link46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Mutagenesis1391K → T: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with Q-193. Ref.3
Mutagenesis1931E → Q: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with T-139. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q505J9 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 358788BA7E7140BC

FASTA36140,717
        10         20         30         40         50         60 
MVHAEAFSRP LSRNEVVGLI FRLTIFGAVT YFTIKWMVDA IDPTRKQKVE AQKQAEKLMK 

        70         80         90        100        110        120 
QIGVKNVKLS EYEMSIAAHL VDPLNMHVTW SDIAGLDDVI TDLKDTVILP IKKKHLFENS 

       130        140        150        160        170        180 
RLLQPPKGVL LYGPPGCGKT LIAKATAKEA GCRFINLQPS TLTDKWYGES QKLAAAVFSL 

       190        200        210        220        230        240 
AIKLQPSIIF IDEIDSFLRN RSSSDHEATA MMKAQFMSLW DGLDTDHSCQ VIVMGATNRP 

       250        260        270        280        290        300 
QDLDSAIMRR MPTRFHINQP ALKQREAILK LILKNENVDR HVDLLEVAQE TDGFSGSDLK 

       310        320        330        340        350        360 
EMCRDAALLC VREYVNSTSE ESHDEDEIRP VQQQDLHRAI EKMKKSKDAA FQSVLTHVCL 


D 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
[2]"Functional identification of neuroprotective molecules."
Dai C., Liang D., Li H., Sasaki M., Dawson T.M., Dawson V.L.
PLoS ONE 5:E15008-E15008(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-361.
[3]"The AAA+ ATPase Thorase regulates AMPA receptor-dependent synaptic plasticity and behavior."
Zhang J., Wang Y., Chi Z., Keuss M.J., Pai Y.M., Kang H.C., Shin J.H., Bugayenko A., Wang H., Xiong Y., Pletnikov M.V., Mattson M.P., Dawson T.M., Dawson V.L.
Cell 145:284-299(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH GRIA2 AND GRIP1, MUTAGENESIS OF LYS-139 AND GLU-193.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC094514 mRNA. Translation: AAH94514.1.
EF688601 mRNA. Translation: ABX10437.1. Different initiation.
RefSeqNP_001030174.1. NM_001035002.1.
UniGeneRn.54918.

3D structure databases

ProteinModelPortalQ505J9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ505J9. 1 interaction.
STRING10116.ENSRNOP00000041122.

PTM databases

PhosphoSiteQ505J9.

Proteomic databases

PaxDbQ505J9.
PRIDEQ505J9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000014684; ENSRNOP00000014684; ENSRNOG00000010861.
GeneID309532.
KEGGrno:309532.
UCSCRGD:1308570. rat.

Organism-specific databases

CTD84896.
RGD1308570. Atad1.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00550000074823.
HOGENOMHOG000225141.
HOVERGENHBG057074.
OMAREMCRDA.
OrthoDBEOG7SR4MJ.

Gene expression databases

GenevestigatorQ505J9.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio660942.
PROQ505J9.

Entry information

Entry nameATAD1_RAT
AccessionPrimary (citable) accession number: Q505J9
Secondary accession number(s): B3STU2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: June 7, 2005
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families