SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q505J9

- ATAD1_RAT

UniProt

Q505J9 - ATAD1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
ATPase family AAA domain-containing protein 1
Gene
Atad1, Npg6
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Kineticsi

  1. KM=43.4 mM for ATP1 Publication

Vmax=11.0 nM/min/mg enzyme

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1408ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATPase activity Source: UniProtKB
  3. protein binding Source: IntAct

GO - Biological processi

  1. learning Source: UniProtKB
  2. memory Source: UniProtKB
  3. negative regulation of synaptic transmission, glutamatergic Source: UniProtKB
  4. positive regulation of receptor internalization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase family AAA domain-containing protein 1 (EC:3.6.1.3)
Alternative name(s):
Thorase
Gene namesi
Name:Atad1
Synonyms:Npg6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi1308570. Atad1.

Subcellular locationi

Peroxisome By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. mitochondrion Source: Ensembl
  3. peroxisomal membrane Source: Ensembl
  4. postsynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Peroxisome, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1391K → T: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with Q-193. 1 Publication
Mutagenesisi193 – 1931E → Q: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with T-139. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361ATPase family AAA domain-containing protein 1
PRO_0000084793Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki46 – 46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ505J9.
PRIDEiQ505J9.

PTM databases

PhosphoSiteiQ505J9.

Expressioni

Gene expression databases

GenevestigatoriQ505J9.

Interactioni

Subunit structurei

Interacts with GRIA2 and GRIP1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts not only its binding to GRIA2 and GRIP1, but also interaction between GRIP1 and GRIA2, leading to AMPAR complex disassembly.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria2P194913EBI-4280289,EBI-77718

Protein-protein interaction databases

IntActiQ505J9. 1 interaction.
STRINGi10116.ENSRNOP00000041122.

Structurei

3D structure databases

ProteinModelPortaliQ505J9.

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00550000074823.
HOGENOMiHOG000225141.
HOVERGENiHBG057074.
OMAiYNCQVIV.
OrthoDBiEOG7SR4MJ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q505J9-1 [UniParc]FASTAAdd to Basket

« Hide

MVHAEAFSRP LSRNEVVGLI FRLTIFGAVT YFTIKWMVDA IDPTRKQKVE    50
AQKQAEKLMK QIGVKNVKLS EYEMSIAAHL VDPLNMHVTW SDIAGLDDVI 100
TDLKDTVILP IKKKHLFENS RLLQPPKGVL LYGPPGCGKT LIAKATAKEA 150
GCRFINLQPS TLTDKWYGES QKLAAAVFSL AIKLQPSIIF IDEIDSFLRN 200
RSSSDHEATA MMKAQFMSLW DGLDTDHSCQ VIVMGATNRP QDLDSAIMRR 250
MPTRFHINQP ALKQREAILK LILKNENVDR HVDLLEVAQE TDGFSGSDLK 300
EMCRDAALLC VREYVNSTSE ESHDEDEIRP VQQQDLHRAI EKMKKSKDAA 350
FQSVLTHVCL D 361
Length:361
Mass (Da):40,717
Last modified:June 7, 2005 - v1
Checksum:i358788BA7E7140BC
GO

Sequence cautioni

The sequence ABX10437.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC094514 mRNA. Translation: AAH94514.1.
EF688601 mRNA. Translation: ABX10437.1. Different initiation.
RefSeqiNP_001030174.1. NM_001035002.1.
UniGeneiRn.54918.

Genome annotation databases

EnsembliENSRNOT00000014684; ENSRNOP00000014684; ENSRNOG00000010861.
GeneIDi309532.
KEGGirno:309532.
UCSCiRGD:1308570. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC094514 mRNA. Translation: AAH94514.1 .
EF688601 mRNA. Translation: ABX10437.1 . Different initiation.
RefSeqi NP_001030174.1. NM_001035002.1.
UniGenei Rn.54918.

3D structure databases

ProteinModelPortali Q505J9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q505J9. 1 interaction.
STRINGi 10116.ENSRNOP00000041122.

PTM databases

PhosphoSitei Q505J9.

Proteomic databases

PaxDbi Q505J9.
PRIDEi Q505J9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000014684 ; ENSRNOP00000014684 ; ENSRNOG00000010861 .
GeneIDi 309532.
KEGGi rno:309532.
UCSCi RGD:1308570. rat.

Organism-specific databases

CTDi 84896.
RGDi 1308570. Atad1.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00550000074823.
HOGENOMi HOG000225141.
HOVERGENi HBG057074.
OMAi YNCQVIV.
OrthoDBi EOG7SR4MJ.

Miscellaneous databases

NextBioi 660942.
PROi Q505J9.

Gene expression databases

Genevestigatori Q505J9.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  2. "Functional identification of neuroprotective molecules."
    Dai C., Liang D., Li H., Sasaki M., Dawson T.M., Dawson V.L.
    PLoS ONE 5:E15008-E15008(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-361.
  3. "The AAA+ ATPase Thorase regulates AMPA receptor-dependent synaptic plasticity and behavior."
    Zhang J., Wang Y., Chi Z., Keuss M.J., Pai Y.M., Kang H.C., Shin J.H., Bugayenko A., Wang H., Xiong Y., Pletnikov M.V., Mattson M.P., Dawson T.M., Dawson V.L.
    Cell 145:284-299(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH GRIA2 AND GRIP1, MUTAGENESIS OF LYS-139 AND GLU-193.

Entry informationi

Entry nameiATAD1_RAT
AccessioniPrimary (citable) accession number: Q505J9
Secondary accession number(s): B3STU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi