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Protein

ATPase family AAA domain-containing protein 1

Gene

Atad1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Kineticsi

  1. KM=43.4 mM for ATP1 Publication
  1. Vmax=11.0 nM/min/mg enzyme1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 140ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase family AAA domain-containing protein 1 (EC:3.6.1.3)
Alternative name(s):
Thorase
Gene namesi
Name:Atad1
Synonyms:Npg6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1308570. Atad1.

Subcellular locationi

  • Peroxisome By similarity
  • Cell junctionsynapsepostsynaptic cell membrane By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Peroxisome, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139K → T: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with Q-193. 1 Publication1
Mutagenesisi193E → Q: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with T-139. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847931 – 361ATPase family AAA domain-containing protein 1Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei322PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ505J9.
PRIDEiQ505J9.

PTM databases

iPTMnetiQ505J9.
PhosphoSitePlusiQ505J9.

Expressioni

Gene expression databases

BgeeiENSRNOG00000010861.
GenevisibleiQ505J9. RN.

Interactioni

Subunit structurei

Interacts with GRIA2 and GRIP1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts not only its binding to GRIA2 and GRIP1, but also interaction between GRIP1 and GRIA2, leading to AMPAR complex disassembly.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria2P194913EBI-4280289,EBI-77718

Protein-protein interaction databases

IntActiQ505J9. 1 interactor.
STRINGi10116.ENSRNOP00000014684.

Structurei

3D structure databases

ProteinModelPortaliQ505J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0737. Eukaryota.
COG0464. LUCA.
HOGENOMiHOG000225141.
HOVERGENiHBG057074.
InParanoidiQ505J9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q505J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHAEAFSRP LSRNEVVGLI FRLTIFGAVT YFTIKWMVDA IDPTRKQKVE
60 70 80 90 100
AQKQAEKLMK QIGVKNVKLS EYEMSIAAHL VDPLNMHVTW SDIAGLDDVI
110 120 130 140 150
TDLKDTVILP IKKKHLFENS RLLQPPKGVL LYGPPGCGKT LIAKATAKEA
160 170 180 190 200
GCRFINLQPS TLTDKWYGES QKLAAAVFSL AIKLQPSIIF IDEIDSFLRN
210 220 230 240 250
RSSSDHEATA MMKAQFMSLW DGLDTDHSCQ VIVMGATNRP QDLDSAIMRR
260 270 280 290 300
MPTRFHINQP ALKQREAILK LILKNENVDR HVDLLEVAQE TDGFSGSDLK
310 320 330 340 350
EMCRDAALLC VREYVNSTSE ESHDEDEIRP VQQQDLHRAI EKMKKSKDAA
360
FQSVLTHVCL D
Length:361
Mass (Da):40,717
Last modified:June 7, 2005 - v1
Checksum:i358788BA7E7140BC
GO

Sequence cautioni

The sequence ABX10437 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC094514 mRNA. Translation: AAH94514.1.
EF688601 mRNA. Translation: ABX10437.1. Different initiation.
RefSeqiNP_001030174.1. NM_001035002.1.
UniGeneiRn.54918.

Genome annotation databases

GeneIDi309532.
KEGGirno:309532.
UCSCiRGD:1308570. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC094514 mRNA. Translation: AAH94514.1.
EF688601 mRNA. Translation: ABX10437.1. Different initiation.
RefSeqiNP_001030174.1. NM_001035002.1.
UniGeneiRn.54918.

3D structure databases

ProteinModelPortaliQ505J9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ505J9. 1 interactor.
STRINGi10116.ENSRNOP00000014684.

PTM databases

iPTMnetiQ505J9.
PhosphoSitePlusiQ505J9.

Proteomic databases

PaxDbiQ505J9.
PRIDEiQ505J9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi309532.
KEGGirno:309532.
UCSCiRGD:1308570. rat.

Organism-specific databases

CTDi84896.
RGDi1308570. Atad1.

Phylogenomic databases

eggNOGiKOG0737. Eukaryota.
COG0464. LUCA.
HOGENOMiHOG000225141.
HOVERGENiHBG057074.
InParanoidiQ505J9.

Miscellaneous databases

PROiQ505J9.

Gene expression databases

BgeeiENSRNOG00000010861.
GenevisibleiQ505J9. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATAD1_RAT
AccessioniPrimary (citable) accession number: Q505J9
Secondary accession number(s): B3STU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: June 7, 2005
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.