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Q505J9

- ATAD1_RAT

UniProt

Q505J9 - ATAD1_RAT

Protein

ATPase family AAA domain-containing protein 1

Gene

Atad1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    ATPase that plays a critical role in regulating the surface expression of AMPA receptors (AMPAR), thereby regulating synaptic plasticity and learning and memory. Required for NMDA-stimulated AMPAR internalization and inhibition of GRIA1 and GRIA2 recycling back to the plasma membrane; these activities are ATPase-dependent.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Kineticsi

    1. KM=43.4 mM for ATP1 Publication

    Vmax=11.0 nM/min/mg enzyme1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi133 – 1408ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. learning Source: UniProtKB
    2. memory Source: UniProtKB
    3. negative regulation of synaptic transmission, glutamatergic Source: UniProtKB
    4. positive regulation of receptor internalization Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATPase family AAA domain-containing protein 1 (EC:3.6.1.3)
    Alternative name(s):
    Thorase
    Gene namesi
    Name:Atad1
    Synonyms:Npg6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1308570. Atad1.

    Subcellular locationi

    Peroxisome By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. mitochondrion Source: Ensembl
    3. peroxisomal membrane Source: Ensembl
    4. postsynaptic membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Peroxisome, Postsynaptic cell membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi139 – 1391K → T: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with Q-193. 1 Publication
    Mutagenesisi193 – 1931E → Q: ATPase activity reduced by 60%-70%. ATPase activity reduced by 92%; when associated with T-139. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361ATPase family AAA domain-containing protein 1PRO_0000084793Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki46 – 46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiQ505J9.
    PRIDEiQ505J9.

    PTM databases

    PhosphoSiteiQ505J9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ505J9.

    Interactioni

    Subunit structurei

    Interacts with GRIA2 and GRIP1 in an ATP-dependent manner. ATAD1-catalyzed ATP hydrolysis disrupts not only its binding to GRIA2 and GRIP1, but also interaction between GRIP1 and GRIA2, leading to AMPAR complex disassembly.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Gria2P194913EBI-4280289,EBI-77718

    Protein-protein interaction databases

    IntActiQ505J9. 1 interaction.
    STRINGi10116.ENSRNOP00000041122.

    Structurei

    3D structure databases

    ProteinModelPortaliQ505J9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00550000074823.
    HOGENOMiHOG000225141.
    HOVERGENiHBG057074.
    OMAiYNCQVIV.
    OrthoDBiEOG7SR4MJ.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q505J9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVHAEAFSRP LSRNEVVGLI FRLTIFGAVT YFTIKWMVDA IDPTRKQKVE    50
    AQKQAEKLMK QIGVKNVKLS EYEMSIAAHL VDPLNMHVTW SDIAGLDDVI 100
    TDLKDTVILP IKKKHLFENS RLLQPPKGVL LYGPPGCGKT LIAKATAKEA 150
    GCRFINLQPS TLTDKWYGES QKLAAAVFSL AIKLQPSIIF IDEIDSFLRN 200
    RSSSDHEATA MMKAQFMSLW DGLDTDHSCQ VIVMGATNRP QDLDSAIMRR 250
    MPTRFHINQP ALKQREAILK LILKNENVDR HVDLLEVAQE TDGFSGSDLK 300
    EMCRDAALLC VREYVNSTSE ESHDEDEIRP VQQQDLHRAI EKMKKSKDAA 350
    FQSVLTHVCL D 361
    Length:361
    Mass (Da):40,717
    Last modified:June 7, 2005 - v1
    Checksum:i358788BA7E7140BC
    GO

    Sequence cautioni

    The sequence ABX10437.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC094514 mRNA. Translation: AAH94514.1.
    EF688601 mRNA. Translation: ABX10437.1. Different initiation.
    RefSeqiNP_001030174.1. NM_001035002.1.
    UniGeneiRn.54918.

    Genome annotation databases

    EnsembliENSRNOT00000014684; ENSRNOP00000014684; ENSRNOG00000010861.
    GeneIDi309532.
    KEGGirno:309532.
    UCSCiRGD:1308570. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC094514 mRNA. Translation: AAH94514.1 .
    EF688601 mRNA. Translation: ABX10437.1 . Different initiation.
    RefSeqi NP_001030174.1. NM_001035002.1.
    UniGenei Rn.54918.

    3D structure databases

    ProteinModelPortali Q505J9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q505J9. 1 interaction.
    STRINGi 10116.ENSRNOP00000041122.

    PTM databases

    PhosphoSitei Q505J9.

    Proteomic databases

    PaxDbi Q505J9.
    PRIDEi Q505J9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000014684 ; ENSRNOP00000014684 ; ENSRNOG00000010861 .
    GeneIDi 309532.
    KEGGi rno:309532.
    UCSCi RGD:1308570. rat.

    Organism-specific databases

    CTDi 84896.
    RGDi 1308570. Atad1.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00550000074823.
    HOGENOMi HOG000225141.
    HOVERGENi HBG057074.
    OMAi YNCQVIV.
    OrthoDBi EOG7SR4MJ.

    Miscellaneous databases

    NextBioi 660942.
    PROi Q505J9.

    Gene expression databases

    Genevestigatori Q505J9.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    2. "Functional identification of neuroprotective molecules."
      Dai C., Liang D., Li H., Sasaki M., Dawson T.M., Dawson V.L.
      PLoS ONE 5:E15008-E15008(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-361.
    3. "The AAA+ ATPase Thorase regulates AMPA receptor-dependent synaptic plasticity and behavior."
      Zhang J., Wang Y., Chi Z., Keuss M.J., Pai Y.M., Kang H.C., Shin J.H., Bugayenko A., Wang H., Xiong Y., Pletnikov M.V., Mattson M.P., Dawson T.M., Dawson V.L.
      Cell 145:284-299(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH GRIA2 AND GRIP1, MUTAGENESIS OF LYS-139 AND GLU-193.

    Entry informationi

    Entry nameiATAD1_RAT
    AccessioniPrimary (citable) accession number: Q505J9
    Secondary accession number(s): B3STU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3