Q505J8 (SYFA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	Farsa
Synonyms:	Farsla

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 508	507	Phenylalanine--tRNA ligase alpha chain		PRO_0000280449
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	193	1	Phosphoserine By similarity
	Modified residue	311	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q505J8 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 78CE0B72EC5BF377	FASTA	508	57,720
	10 20 30 40 50 60 MADNPVLEQL LRRLEVADGG LDSAELATQL GVEHQAVVGA VKSLQALGEV IEAELRSTKC 70 80 90 100 110 120 WELTTEGEEI AREGSHEARV FRSIPLEGLV QSELMQLPSG KVGFSKAMSN KWIRVDKSAA 130 140 150 160 170 180 DGPRVFRVVD SIEDEVQRRL QQVQAGQAEK LAEKERNELR KRKLLTEVIL KTYWVSKGKG 190 200 210 220 230 240 FSTSVSKQEA ELSPEMISSG SWRDRPFKPY NFSARGVLPD SGHLHPLLKV RSQFRQIFLE 250 260 270 280 290 300 MGFTEMPTDN FIESSFWNFD ALFQPQQHPA RDQHDTFFLR DPAEALQLPM DYVQRVKRTH 310 320 330 340 350 360 SQGGYGSQGY KYTWKLEEAR KNLLRTHTTA ASARALYRLA QKKPFTPAKY FSIDRVFRNE 370 380 390 400 410 420 TLDATHLAEF HQIEGVIADH GLTLGHLMGV LREFFTKLGI TQLRFKPAYN PYTEPSMEVF 430 440 450 460 470 480 SYHQGLKKWV EVGNSGVFRP EMLLPMGLPE NVSVIAWGLS LERPTMIKYG INNIRELVGH 490 500 KVNLQMVYDS PVCRLDIEPR SSKTQEAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12 AND 43-59, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC094515 mRNA. Translation: AAH94515.1.
IPI	IPI00199865.
RefSeq	NP_001019408.1. NM_001024237.1.
UniGene	Rn.2972.
3D structure databases
HSSP	HSSP built from PDB template 2ALY based on UniProtKB P27001.
ProteinModelPortal	Q505J8.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4579145.
STRING	Q505J8.
Proteomic databases
PRIDE	Q505J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000004370; ENSRNOP00000004370; ENSRNOG00000003149.
GeneID	288917.
KEGG	rno:288917.
NMPDR	fig|10116.3.peg.14747.
UCSC	NM_001024237. rat.
Organism-specific databases
CTD	2193.
RGD	1310314. Farsa.
Phylogenomic databases
eggNOG	roNOG05416.
GeneTree	ENSGT00390000006387.
HOVERGEN	HBG068046.
InParanoid	Q505J8.
OMA	FKPYNFL.
OrthoDB	EOG4CZBFN.
PhylomeDB	Q505J8.
Gene expression databases
ArrayExpress	Q505J8.
Genevestigator	Q505J8.
Family and domain databases
InterPro	IPR006195. aa-tRNA-synth_II. IPR013196. HTH_11. IPR004529. Phe-tRNA-synth_IIc_asu. IPR002319. Phenylalanyl-tRNA_Synthase. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01889.
Pfam	PF08279. HTH_11. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	628972.

Entry information

Entry name

SYFA_RAT

Accession

Primary (citable) accession number: Q505J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program