Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A

Gene

Ankrd28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation (By similarity).By similarity

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
Short name:
PP6-ARS-A
Short name:
Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A
Alternative name(s):
Ankyrin repeat domain-containing protein 28
Phosphatase interactor targeting protein hnRNP K
Short name:
PITK
Gene namesi
Name:Ankrd28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2145661. Ankrd28.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10531053Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit APRO_0000244571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1007 – 10071PhosphoserineBy similarity
Modified residuei1011 – 10111PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ505D1.
MaxQBiQ505D1.
PaxDbiQ505D1.
PeptideAtlasiQ505D1.
PRIDEiQ505D1.

PTM databases

iPTMnetiQ505D1.
PhosphoSiteiQ505D1.

Expressioni

Tissue specificityi

Widely expressed (at protein level).1 Publication

Gene expression databases

BgeeiQ505D1.
CleanExiMM_ANKRD28.
GenevisibleiQ505D1. MM.

Interactioni

Subunit structurei

Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). Interacts with PPP1C and HNRPK. Interacts with PPP6C, PPP6R1 and PPP6R3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi222872. 9 interactions.
IntActiQ505D1. 9 interactions.
STRINGi10090.ENSMUSP00000014640.

Structurei

3D structure databases

ProteinModelPortaliQ505D1.
SMRiQ505D1. Positions 8-999.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati40 – 6930ANK 1Add
BLAST
Repeati73 – 10230ANK 2Add
BLAST
Repeati106 – 13530ANK 3Add
BLAST
Repeati139 – 16830ANK 4Add
BLAST
Repeati172 – 20130ANK 5Add
BLAST
Repeati205 – 23430ANK 6Add
BLAST
Repeati238 – 26730ANK 7Add
BLAST
Repeati271 – 30131ANK 8Add
BLAST
Repeati305 – 33430ANK 9Add
BLAST
Repeati338 – 36730ANK 10Add
BLAST
Repeati371 – 40030ANK 11Add
BLAST
Repeati404 – 43330ANK 12Add
BLAST
Repeati437 – 46630ANK 13Add
BLAST
Repeati470 – 50031ANK 14Add
BLAST
Repeati504 – 53431ANK 15Add
BLAST
Repeati549 – 57830ANK 16Add
BLAST
Repeati582 – 61130ANK 17Add
BLAST
Repeati616 – 64530ANK 18Add
BLAST
Repeati652 – 68130ANK 19Add
BLAST
Repeati685 – 71430ANK 20Add
BLAST
Repeati718 – 74730ANK 21Add
BLAST
Repeati755 – 78632ANK 22Add
BLAST
Repeati788 – 81730ANK 23Add
BLAST
Repeati822 – 85130ANK 24Add
BLAST
Repeati855 – 88531ANK 25Add
BLAST
Repeati889 – 91830ANK 26Add
BLAST
Repeati925 – 95430ANK 27Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi987 – 9904Poly-Ser

Sequence similaritiesi

Contains 27 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129677.
HOGENOMiHOG000033959.
HOVERGENiHBG067697.
InParanoidiQ505D1.
KOiK15502.
OMAiQKMEGNS.
OrthoDBiEOG7QG436.
PhylomeDBiQ505D1.
TreeFamiTF312824.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF12796. Ank_2. 7 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 28 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 4 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 24 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q505D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLKLRDQP SLVQAIFNGD PDEVRALIFK KEDVNFQDNE KRTPLHAAAY
60 70 80 90 100
LGDAEIIELL ILSGARVNAK DSKWLTPLHR AVASCSEEAV QILLKHSADV
110 120 130 140 150
NARDKNWQTP LHIAAANKAV KCAESLVPLL SNVNVSDRAG RTALHHAAFS
160 170 180 190 200
GHGEMVKLLL SRGANINAFD KKDRRAIHWA AYMGHIEVVK LLVSHGAEVT
210 220 230 240 250
CKDKKSYTPL HAAASSGMIS VVKYLLDLGV DMNEPNAYGN TPLHVACYNG
260 270 280 290 300
QDVVVNELID CGANVNQKNE KGFTPLHFAA ASTHGALCLE LLVGNGADVN
310 320 330 340 350
MKSKDGKTPL HMTALHGRFS RSQTIIQSGA VIDCEDKNGN TPLHIAARYG
360 370 380 390 400
HELLINTLIT SGADTAKRGI HGMFPLHLAA LSGFSDCCRK LLSSGFDIDT
410 420 430 440 450
PDDFGRTCLH AAAAGGNLEC LNLLLNTGAD FNKKDKFGRS PLHYAAANCN
460 470 480 490 500
YQCLFALVGS GASVNDLDER GCTPLHYAAT SDTDGKCLEY LLRNDANPGI
510 520 530 540 550
RDKQGYNAVH YSAAYGHRLC LQLIASETPL DVLMETSGTD MLSDSDNRAT
560 570 580 590 600
ISPLHLAAYH GHHQALEVLV QSLLDLDVRN SSGRTPLDLA AFKGHVECVD
610 620 630 640 650
VLINQGASIL VKDYVLKRTP IHAAATNGHS ECLRLLIGNA EPQNAVDIQD
660 670 680 690 700
GNGQTPLMLS VLNGHTDCVY SLLNKGANVD AKDKWGRTAL HRGAVTGHEE
710 720 730 740 750
CVDALLQHGA KCLLRDSRGR TPIHLSAACG HIGVLGALLQ SATSVDANPA
760 770 780 790 800
VVDNHGYTAL HWACYNGHET CVELLLEQDV FQKIDGNAFS PLHCAVINDN
810 820 830 840 850
EGAAEMLIDS LGASIVNATD SKGRTPLHAA AFTDHVECLQ LLLSQNAQVN
860 870 880 890 900
SADSTGKTPL MMAAENGQTN TVEMLVSSAS ADLTLQDKSK NTALHLACGK
910 920 930 940 950
GHETSALLIL EKITDRNLIN ATNAALQTPL HVAARNGLTM VVQELLGKGA
960 970 980 990 1000
SVLAVDENGY TPALACAPNK DVADCLALIL ATMMPVSSSS PLTSLTFNAI
1010 1020 1030 1040 1050
NRYTNTSKTV SFEALPIMRN EASSYCSFNN IGGEQEYLYT DVDELNDSDS

ETY
Length:1,053
Mass (Da):112,898
Last modified:June 7, 2005 - v1
Checksum:iD7649AF1D1F22F62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC094609 mRNA. Translation: AAH94609.1.
BC051456 mRNA. Translation: AAH51456.1.
CCDSiCCDS36858.1.
RefSeqiNP_001019775.1. NM_001024604.2.
UniGeneiMm.37660.

Genome annotation databases

EnsembliENSMUST00000014640; ENSMUSP00000014640; ENSMUSG00000014496.
GeneIDi105522.
KEGGimmu:105522.
UCSCiuc007sxz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC094609 mRNA. Translation: AAH94609.1.
BC051456 mRNA. Translation: AAH51456.1.
CCDSiCCDS36858.1.
RefSeqiNP_001019775.1. NM_001024604.2.
UniGeneiMm.37660.

3D structure databases

ProteinModelPortaliQ505D1.
SMRiQ505D1. Positions 8-999.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222872. 9 interactions.
IntActiQ505D1. 9 interactions.
STRINGi10090.ENSMUSP00000014640.

PTM databases

iPTMnetiQ505D1.
PhosphoSiteiQ505D1.

Proteomic databases

EPDiQ505D1.
MaxQBiQ505D1.
PaxDbiQ505D1.
PeptideAtlasiQ505D1.
PRIDEiQ505D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014640; ENSMUSP00000014640; ENSMUSG00000014496.
GeneIDi105522.
KEGGimmu:105522.
UCSCiuc007sxz.1. mouse.

Organism-specific databases

CTDi23243.
MGIiMGI:2145661. Ankrd28.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129677.
HOGENOMiHOG000033959.
HOVERGENiHBG067697.
InParanoidiQ505D1.
KOiK15502.
OMAiQKMEGNS.
OrthoDBiEOG7QG436.
PhylomeDBiQ505D1.
TreeFamiTF312824.

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.

Miscellaneous databases

PROiQ505D1.
SOURCEiSearch...

Gene expression databases

BgeeiQ505D1.
CleanExiMM_ANKRD28.
GenevisibleiQ505D1. MM.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF12796. Ank_2. 7 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 28 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 4 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 24 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain and Olfactory epithelium.
  2. "PITK, a PP1 targeting subunit that modulates the phosphorylation of the transcriptional regulator hnRNP K."
    Kwiek N.C., Thacker D.F., Datto M.B., Megosh H.B., Haystead T.A.J.
    Cell. Signal. 18:1769-1778(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1011, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Pancreas and Testis.

Entry informationi

Entry nameiANR28_MOUSE
AccessioniPrimary (citable) accession number: Q505D1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 7, 2005
Last modified: July 6, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.