Q50570 (FDHB_METTF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate dehydrogenase subunit beta
EC=1.2.1.2

Gene names

Name:

fdhB

Organism

Methanobacterium thermoformicicum

Taxonomic identifier

145262 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of formate.
Catalytic activity	Formate + NAD+ = CO2 + NADH.
Cofactor	Binds 2 4Fe-4S clusters Probable.
Subunit structure	Dimer of an alpha and a beta subunit.
Sequence similarities	Belongs to the frhB family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Formate dehydrogenase subunit beta		PRO_0000159223
Regions
	Domain	285 – 315	31	4Fe-4S ferredoxin-type 1
	Domain	337 – 367	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	295	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	298	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	301	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	305	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	346	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	349	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	352	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	356	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q50570 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F95B3E85B4C44316	FASTA	394	43,683
	10 20 30 40 50 60 MVEVNDMYYA FSKNEEIAEK GEYGGAVTSL LKFLLEEGMV DAVLAVKKGS DLYDAVPTLI 70 80 90 100 110 120 TEPEKVIESA GSLHCGTLNI AKVITRYLNG AKDIKIAVTT KPCDAMTIVE VAKRGKIDLD 130 140 150 160 170 180 NVIMVGVNCG GTLPPVKTRA MIEEVYEMDP DDVVKEEIAK GKLIIETSDA EKGISVDEVE 190 200 210 220 230 240 EMGYGRRTNC RRCEKNIPRM ADLALGNWGV IGPLAGKATF VEVTSEKGAD LLEKAVSAGV 250 260 270 280 290 300 IEIEDPIPKG IEIREKIDQA MVNLAKKWQK NDFNEETGAE ILMNLDRYMD ELNKCIKCYS 310 320 330 340 350 360 CREACPICYC EECSLETKTP EWIEKGKIPP SPLFHLERML HMVDSCTNCG QCEELCPAEI 370 380 390 PLAKIWHEIN CRVQETFGYK TGFETGQEPP LTHP

References

[1]	"Growth- and substrate-dependent transcription of the formate dehydrogenase (fdhCAB) operon in Methanobacterium thermoformicicum Z-245." Noelling J., Reeve J.N. J. Bacteriol. 179:899-908(1997) [PubMed: 9006048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 3720 / Z-245.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52681 Genomic DNA. Translation: AAC44821.1.
3D structure databases
ProteinModelPortal	Q50570.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR007516. Co_F420_Hydgase/DH_bsu_N. IPR007525. FrhB_FdhB_C. IPR012285. Fum_reductase_C. IPR009051. Helical_ferredxn. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF04432. FrhB_FdhB_C. 1 hit. PF04422. FrhB_FdhB_N. 1 hit. [Graphical view]
SUPFAM	SSF46548. Helical_ferredxn. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDHB_METTF

Accession

Primary (citable) accession number: Q50570

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program